Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   E3Z29_RS04640 Genome accession   NZ_CP038207
Coordinates   1000713..1001777 (+) Length   354 a.a.
NCBI ID   WP_096794691.1    Uniprot ID   -
Organism   Pseudomonas sp. S150     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 995713..1006777
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  E3Z29_RS04630 (E3Z29_04630) - 996518..997786 (+) 1269 WP_003220889.1 malic enzyme-like NAD(P)-binding protein -
  E3Z29_RS04635 (E3Z29_04635) - 998049..1000493 (-) 2445 WP_372241217.1 penicillin-binding protein 1A -
  E3Z29_RS04640 (E3Z29_04640) pilM 1000713..1001777 (+) 1065 WP_096794691.1 pilus assembly protein PilM Machinery gene
  E3Z29_RS04645 (E3Z29_04645) - 1001777..1002343 (+) 567 WP_134785244.1 PilN domain-containing protein -
  E3Z29_RS04650 (E3Z29_04650) pilO 1002340..1002963 (+) 624 WP_098966813.1 type 4a pilus biogenesis protein PilO -
  E3Z29_RS04655 (E3Z29_04655) - 1002960..1003487 (+) 528 WP_134785245.1 pilus assembly protein PilP -
  E3Z29_RS04660 (E3Z29_04660) pilQ 1003501..1005579 (+) 2079 WP_134785246.1 type IV pilus secretin PilQ Machinery gene
  E3Z29_RS04665 (E3Z29_04665) aroK 1005584..1006102 (+) 519 WP_134785247.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 354 a.a.        Molecular weight: 38104.68 Da        Isoelectric Point: 4.9026

>NTDB_id=352294 E3Z29_RS04640 WP_096794691.1 1000713..1001777(+) (pilM) [Pseudomonas sp. S150]
MLGLFKKKTNTLLGIDISSTSVKLLELSRQGDRYRVEAYAVEPLPPNAVVEKNIAELEGVGHALSRVLVKARTGLKSVAV
AVAGSAVITKIIEMDAGLTDDELENQLKIEADQYIPYPLDEVAIDFEVQGVSPRNPERVSVLLAACRKENVEVREAALAL
AGLTARVVDVEAYALERSFGLLATQLAASQERLTVAVVDIGATMTTLSVLHNGRIIYTREQLFGGRQLTEEIQRRYGLTV
EQAGLAKKQGGLPDDYVSEVLQPFRDALVQQVSRSLQFFFASGQYNAVDHILLAGGTASVPGLDRLIEQRLGTPTQVANP
FSDMALSSKVNAGALASDAPALMIACGLALRSFD

Nucleotide


Download         Length: 1065 bp        

>NTDB_id=352294 E3Z29_RS04640 WP_096794691.1 1000713..1001777(+) (pilM) [Pseudomonas sp. S150]
GTGCTGGGACTCTTCAAGAAAAAGACCAATACGTTACTGGGGATCGACATCAGCTCCACTTCGGTGAAGCTGTTGGAGTT
GAGCCGTCAGGGCGACCGCTACCGGGTCGAAGCCTACGCGGTCGAGCCGCTGCCGCCCAATGCCGTTGTCGAAAAGAACA
TCGCCGAGCTCGAAGGCGTGGGCCATGCCTTGTCCCGGGTGCTGGTCAAGGCACGCACCGGGCTCAAGAGCGTGGCGGTG
GCGGTGGCCGGTTCTGCGGTGATCACCAAGATCATCGAGATGGACGCCGGGCTGACCGATGACGAGCTGGAAAACCAGCT
GAAGATCGAAGCCGACCAATACATTCCCTATCCACTGGACGAAGTCGCCATCGATTTCGAAGTCCAGGGCGTGTCGCCGC
GCAACCCGGAGCGGGTCAGCGTGCTGCTGGCCGCCTGTCGCAAGGAAAACGTCGAAGTCCGCGAAGCGGCGCTGGCGCTG
GCCGGGCTGACCGCGCGCGTGGTCGATGTCGAGGCCTATGCGCTGGAGCGCTCGTTCGGCCTGCTCGCCACTCAACTGGC
AGCCTCGCAGGAACGCCTGACCGTGGCGGTGGTGGACATCGGCGCGACCATGACCACCCTCAGCGTCCTGCACAACGGCC
GCATCATCTATACCCGTGAGCAGCTGTTCGGTGGCAGGCAGCTGACTGAGGAGATCCAGCGCCGCTACGGCCTGACCGTC
GAGCAGGCCGGGCTGGCGAAAAAGCAGGGCGGTCTGCCGGACGACTATGTCAGCGAGGTATTGCAACCGTTCCGTGATGC
TCTGGTGCAGCAGGTCTCGCGCTCGTTGCAGTTCTTCTTTGCCTCCGGGCAGTACAACGCGGTCGATCACATTCTGCTGG
CCGGCGGCACCGCCTCGGTGCCGGGGCTGGATCGGCTGATCGAACAGCGTCTGGGCACCCCGACCCAGGTCGCCAACCCG
TTTTCCGACATGGCCTTGAGCAGCAAGGTCAACGCCGGGGCCCTGGCCAGTGACGCGCCGGCCCTGATGATTGCCTGCGG
GCTCGCGCTCAGGAGTTTCGACTGA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(14-353)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Acinetobacter baumannii D1279779

56.215

100

0.562
  comM Acinetobacter nosocomialis M2

56.215

100

0.562
  comM Acinetobacter baylyi ADP1

55.367

100

0.554
  pilM Legionella pneumophila strain ERS1305867

48.588

100

0.486

Multiple sequence alignment