Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   E3U44_RS04820 Genome accession   NZ_CP038033
Coordinates   992931..994646 (-) Length   571 a.a.
NCBI ID   WP_206054890.1    Uniprot ID   -
Organism   Nitrosococcus wardiae strain D1FHS     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 987931..999646
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  E3U44_RS04785 (E3U44_04785) - 987946..988146 (-) 201 WP_134356913.1 DUF2905 domain-containing protein -
  E3U44_RS04790 (E3U44_04790) - 988188..989138 (+) 951 WP_134356914.1 Nudix family hydrolase -
  E3U44_RS04795 (E3U44_04795) - 989144..989332 (-) 189 WP_134356915.1 DNA gyrase inhibitor YacG -
  E3U44_RS04800 (E3U44_04800) zapD 989329..990096 (-) 768 WP_134356916.1 cell division protein ZapD -
  E3U44_RS04805 (E3U44_04805) coaE 990169..990768 (-) 600 WP_134356917.1 dephospho-CoA kinase -
  E3U44_RS04810 (E3U44_04810) pilD 990817..991686 (-) 870 WP_134356918.1 prepilin peptidase Machinery gene
  E3U44_RS04815 (E3U44_04815) pilC 991706..992926 (-) 1221 WP_134356919.1 type II secretion system F family protein Machinery gene
  E3U44_RS04820 (E3U44_04820) pilB 992931..994646 (-) 1716 WP_206054890.1 type IV-A pilus assembly ATPase PilB Machinery gene
  E3U44_RS04825 (E3U44_04825) lptM 994811..994996 (+) 186 WP_206054891.1 LPS translocon maturation chaperone LptM -
  E3U44_RS04830 (E3U44_04830) lysA 995000..996250 (+) 1251 WP_134356920.1 diaminopimelate decarboxylase -
  E3U44_RS04835 (E3U44_04835) - 996311..996982 (+) 672 WP_134356921.1 class I SAM-dependent methyltransferase -
  E3U44_RS04840 (E3U44_04840) dapF 997021..997851 (+) 831 WP_134356922.1 diaminopimelate epimerase -
  E3U44_RS04845 (E3U44_04845) - 997848..998579 (+) 732 WP_134356923.1 DUF484 family protein -
  E3U44_RS04850 (E3U44_04850) xerC 998586..999488 (+) 903 WP_134356924.1 tyrosine recombinase XerC -

Sequence

Protein


Download         Length: 571 a.a.        Molecular weight: 62750.99 Da        Isoelectric Point: 5.1434

>NTDB_id=351653 E3U44_RS04820 WP_206054890.1 992931..994646(-) (pilB) [Nitrosococcus wardiae strain D1FHS]
MATPGAQIIMNGLARRLVEDGLLSEADALQAHEQSRKASIPFVTYLVQQKLLKSLDISQAASQEFGIPLFDLDALDLDCL
PKGLVEEKLIRQHQALPIFKRGNRLFVAVADPTNLQALDEIKFHAGINTEAILVEEDKLAQTIDRAMEAQDTSLADLNDT
ALDDLDISGGEEETPQNSIESDVDDTPVVRFINKALLDAIHQGASDIHFEPYEKIYRIRYRQDGVLREVVCPPVSLASRL
AARLKVMARLDISERRVPQDGRMKMNISKNRAIDFRVNTCPTLFGEKIVLRILDPSSAQLGIDALGYEEKQKQIFLETIH
RPYGMVLVTGPTGSGKTVSLYTALNILNTADRNISTAEDPAEINLPGINQVNVYPKVGLTFAGALRAFLRQDPDVIMVGE
IRDLETAEIAIKAAQTGHMVLSTLHTNDAPQTLTRLLNMGVASYNIASAVSLIIAQRLARRLCSRCKAPEKIPQEALLEE
GFTQAQLDASPTIYKAVGCQRCSGGYKGRVGIYQVMPVSEEMGRIIMAGGNSMELSEQAKKEGVVDLRQSGLKKVIDGIT
SLEEINRVTKE

Nucleotide


Download         Length: 1716 bp        

>NTDB_id=351653 E3U44_RS04820 WP_206054890.1 992931..994646(-) (pilB) [Nitrosococcus wardiae strain D1FHS]
ATGGCAACCCCTGGAGCACAGATCATAATGAACGGTCTGGCCCGCCGTTTAGTGGAAGATGGGCTACTTAGCGAAGCGGA
TGCCCTGCAGGCTCATGAGCAGTCCCGCAAGGCCAGTATTCCTTTTGTCACTTACTTAGTCCAGCAAAAACTACTCAAAA
GCCTGGATATTAGCCAAGCGGCCTCACAGGAATTTGGCATTCCTCTCTTCGATCTCGATGCGTTGGATTTGGACTGCCTT
CCCAAAGGCCTGGTGGAAGAAAAACTAATCCGCCAGCATCAAGCATTACCCATTTTCAAGCGCGGGAATCGCCTGTTCGT
TGCCGTGGCCGATCCCACCAACCTCCAGGCATTAGACGAAATTAAATTCCACGCGGGGATTAATACCGAAGCTATCCTCG
TTGAAGAAGATAAGCTAGCACAAACTATTGATCGGGCCATGGAGGCCCAAGACACTTCCCTTGCCGATCTGAATGATACC
GCATTAGATGATCTCGACATTTCCGGGGGCGAAGAGGAGACCCCGCAAAATTCTATCGAATCTGACGTCGATGATACACC
TGTAGTACGCTTTATTAATAAGGCCCTATTGGATGCCATTCATCAGGGGGCCTCGGATATCCATTTTGAGCCCTATGAGA
AAATCTATCGGATTAGATACCGCCAAGATGGGGTGCTGCGGGAAGTCGTTTGCCCTCCCGTAAGCCTAGCAAGCCGGCTA
GCAGCTCGCCTTAAGGTGATGGCCCGCTTGGATATTTCCGAACGGCGCGTGCCCCAAGATGGGCGCATGAAAATGAATAT
CTCCAAAAACCGAGCAATTGACTTTCGTGTCAATACCTGTCCTACTTTATTTGGTGAGAAAATCGTCCTTCGTATCCTGG
ACCCCTCCAGCGCCCAATTGGGAATCGACGCCCTCGGTTATGAAGAAAAACAAAAACAAATCTTTCTAGAAACTATCCAC
CGGCCTTACGGGATGGTGCTGGTTACTGGCCCCACTGGCAGCGGTAAAACCGTCTCTCTTTATACCGCGCTGAATATTCT
TAATACCGCGGACCGTAATATCTCCACCGCCGAGGATCCGGCCGAAATCAACCTGCCCGGCATTAATCAGGTTAACGTAT
ACCCGAAGGTAGGGTTGACCTTTGCTGGAGCCCTAAGAGCCTTTTTACGTCAAGATCCTGATGTCATCATGGTAGGTGAG
ATTCGGGATCTCGAAACGGCTGAAATTGCCATCAAAGCGGCCCAAACCGGTCATATGGTGCTTTCCACCCTCCACACCAA
TGACGCACCCCAGACCCTGACTCGGTTATTAAACATGGGGGTGGCTTCCTACAACATTGCCTCGGCAGTCTCTCTGATTA
TTGCCCAGCGCTTGGCACGGCGCCTTTGTTCCCGATGTAAAGCTCCCGAGAAGATCCCTCAGGAAGCCTTATTAGAAGAA
GGTTTCACCCAAGCCCAGCTCGACGCCTCACCCACTATTTATAAAGCAGTAGGCTGCCAGCGCTGCTCAGGAGGCTATAA
GGGCCGAGTGGGCATCTATCAAGTCATGCCGGTATCCGAGGAGATGGGGCGCATCATTATGGCAGGGGGCAACTCCATGG
AGCTCTCGGAGCAAGCTAAAAAGGAAGGAGTGGTCGATCTACGCCAGTCTGGGCTCAAAAAAGTTATTGATGGGATTACC
AGTTTAGAGGAAATCAACCGCGTGACGAAGGAGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(194-461)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(46-152)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

58.421

99.825

0.583
  pilB Acinetobacter baylyi ADP1

57.42

99.124

0.569
  pilB Legionella pneumophila strain ERS1305867

55.652

100

0.56
  pilF Neisseria gonorrhoeae MS11

52.021

99.65

0.518
  pilB Vibrio cholerae strain A1552

51.859

94.221

0.489
  pilB Vibrio parahaemolyticus RIMD 2210633

48.94

99.124

0.485
  pilB Vibrio campbellii strain DS40M4

48.41

99.124

0.48
  pilF Thermus thermophilus HB27

39.459

97.198

0.384
  pilB/pilB1 Synechocystis sp. PCC 6803

41.892

90.718

0.38

Multiple sequence alignment