Detailed information NTG0351651

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: E3U44_RS04810
• Genome accession: NZ_CP038033
• Coordinates: 990817..991686 (-)
• Length: 289 a.a.
• NCBI ID: WP_134356918.1
• Uniprot ID: A0A4P7BXJ5
• Organism: Nitrosococcus wardiae strain D1FHS
• Function: type IV pilus biogenesis and function (predicted from homology) DNA binding and uptake

Genomic Context

Location: 985817..996686

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
E3U44_RS04775 (E3U44_04775)	nadA	986475..987572 (+)	1098	WP_134356911.1	quinolinate synthase NadA	-
E3U44_RS04780 (E3U44_04780)	-	987629..987856 (+)	228	WP_134356912.1	zinc-finger domain-containing protein	-
E3U44_RS04785 (E3U44_04785)	-	987946..988146 (-)	201	WP_134356913.1	DUF2905 domain-containing protein	-
E3U44_RS04790 (E3U44_04790)	-	988188..989138 (+)	951	WP_134356914.1	Nudix family hydrolase	-
E3U44_RS04795 (E3U44_04795)	-	989144..989332 (-)	189	WP_134356915.1	DNA gyrase inhibitor YacG	-
E3U44_RS04800 (E3U44_04800)	zapD	989329..990096 (-)	768	WP_134356916.1	cell division protein ZapD	-
E3U44_RS04805 (E3U44_04805)	coaE	990169..990768 (-)	600	WP_134356917.1	dephospho-CoA kinase	-
E3U44_RS04810 (E3U44_04810)	pilD	990817..991686 (-)	870	WP_134356918.1	prepilin peptidase	Machinery gene
E3U44_RS04815 (E3U44_04815)	pilC	991706..992926 (-)	1221	WP_134356919.1	type II secretion system F family protein	Machinery gene
E3U44_RS04820 (E3U44_04820)	pilB	992931..994646 (-)	1716	WP_206054890.1	type IV-A pilus assembly ATPase PilB	Machinery gene
E3U44_RS04825 (E3U44_04825)	lptM	994811..994996 (+)	186	WP_206054891.1	LPS translocon maturation chaperone LptM	-
E3U44_RS04830 (E3U44_04830)	lysA	995000..996250 (+)	1251	WP_134356920.1	diaminopimelate decarboxylase	-

Sequence

Protein

• Length: 289 a.a.
• Molecular weight: 32341.20 Da
• Isoelectric Point: 6.9532

>NTDB_id=351651 E3U44_RS04810 WP_134356918.1 990817..991686(-) (pilD) [Nitrosococcus wardiae strain D1FHS]
MEVIAFFENHLIAFVASAFVLGLVVGSFLNVVIYRLPLMMERQWKSQCAELHGKSLPEQEPFNLFVPRSRCPACRHKIQA
WENIPLFSYLLLLGRCNHCGASISPRYPLVELLTGVLSAAVAWHFGTSWETIAALLLSYALITLTFIDFDHQLLPDSITL
PFLWFGLGLSLFEVFTDVRTSLIGAMAGYLSLWLVYHLFRLLTKKEGMGYGDFKLLALLGAWLGWTMLPAIILLSSLVGA
LLGTIWLSLSSQHRETPLPFGPYLAAAGWLALMWGQDINHFYLSFTGLG

Nucleotide

• Length: 870 bp

>NTDB_id=351651 E3U44_RS04810 WP_134356918.1 990817..991686(-) (pilD) [Nitrosococcus wardiae strain D1FHS]
ATGGAAGTTATTGCCTTTTTTGAGAATCATCTGATTGCCTTTGTGGCTTCAGCCTTTGTACTAGGCTTAGTGGTAGGCAG
TTTCCTTAATGTGGTGATTTACCGCCTTCCCCTCATGATGGAAAGACAGTGGAAGAGCCAGTGTGCGGAACTGCATGGTA
AGTCCCTACCGGAGCAGGAACCATTCAACCTGTTCGTCCCCCGCTCTCGCTGTCCAGCGTGTCGCCACAAAATACAAGCT
TGGGAGAACATTCCCTTATTCAGTTATCTCCTGTTACTGGGACGCTGCAATCACTGCGGCGCTTCTATTTCCCCCCGCTA
TCCCCTGGTAGAGCTTTTAACCGGCGTGCTTTCTGCTGCCGTAGCTTGGCACTTTGGCACCAGCTGGGAAACCATTGCCG
CTTTGCTCTTGAGTTATGCCCTTATTACGCTTACCTTTATTGATTTTGATCACCAACTGCTGCCCGACAGTATTACCCTT
CCTTTTCTTTGGTTTGGATTAGGACTAAGTTTATTTGAGGTATTTACGGATGTCCGAACCAGCCTTATTGGCGCCATGGC
AGGCTATCTTTCCCTATGGCTGGTTTACCATCTGTTTCGGCTGCTTACCAAAAAAGAGGGAATGGGATATGGCGACTTCA
AACTTTTAGCACTCCTTGGTGCCTGGTTGGGCTGGACGATGCTCCCTGCTATCATTCTACTTTCCTCACTGGTAGGCGCC
CTACTGGGTACCATATGGCTCTCTCTTTCCAGCCAACACCGAGAAACCCCCCTCCCCTTTGGCCCTTATCTTGCAGCTGC
GGGTTGGTTAGCCTTGATGTGGGGACAGGATATCAACCATTTTTATCTCTCCTTCACCGGACTCGGCTAA

Domains

Predicted by InterproScan.

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (20-125)

• Type IV leader peptidase family (Peptidase_A24) (136-243)

3D structure

• AlphaFold DB: A0A4P7BXJ5

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio campbellii strain DS40M4	53.659	99.308	0.533
pilD	Vibrio cholerae strain A1552	54.71	95.502	0.522
pilD	Acinetobacter nosocomialis M2	48.944	98.27	0.481
pilD	Acinetobacter baumannii D1279779	48.592	98.27	0.478
pilD	Neisseria gonorrhoeae MS11	52.157	88.235	0.46