Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   E1742_RS21270 Genome accession   NZ_CP038026
Coordinates   4789722..4791446 (-) Length   574 a.a.
NCBI ID   WP_134387125.1    Uniprot ID   -
Organism   Pseudoduganella plicata strain DSM 17505     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4784722..4796446
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  E1742_RS21240 (E1742_21240) - 4785046..4785729 (+) 684 WP_134387119.1 4'-phosphopantetheinyl transferase -
  E1742_RS21245 (E1742_21245) yacG 4785757..4785933 (-) 177 WP_134387120.1 DNA gyrase inhibitor YacG -
  E1742_RS21250 (E1742_21250) zapD 4785946..4786701 (-) 756 WP_134387121.1 cell division protein ZapD -
  E1742_RS21255 (E1742_21255) coaE 4786900..4787526 (-) 627 WP_134387122.1 dephospho-CoA kinase -
  E1742_RS21260 (E1742_21260) pilD 4787526..4788401 (-) 876 WP_134387123.1 A24 family peptidase Machinery gene
  E1742_RS21265 (E1742_21265) pilC 4788485..4789714 (-) 1230 WP_134387124.1 type II secretion system F family protein Machinery gene
  E1742_RS21270 (E1742_21270) pilB 4789722..4791446 (-) 1725 WP_134387125.1 type IV-A pilus assembly ATPase PilB Machinery gene
  E1742_RS21275 (E1742_21275) - 4791556..4792827 (-) 1272 WP_134387126.1 HlyC/CorC family transporter -
  E1742_RS21280 (E1742_21280) - 4792997..4794226 (+) 1230 WP_134387127.1 glutamate carboxypeptidase -
  E1742_RS21285 (E1742_21285) uraH 4794211..4794564 (-) 354 WP_134387128.1 hydroxyisourate hydrolase -
  E1742_RS21290 (E1742_21290) - 4794656..4796197 (+) 1542 WP_229466167.1 carboxylesterase/lipase family protein -

Sequence

Protein


Download         Length: 574 a.a.        Molecular weight: 61813.90 Da        Isoelectric Point: 6.2756

>NTDB_id=351543 E1742_RS21270 WP_134387125.1 4789722..4791446(-) (pilB) [Pseudoduganella plicata strain DSM 17505]
MAAVQPTTAAGAPMSGLARALMQAGRLTPPQADALLKKSILEKLPFIDVLLASGAIGGRELAVFCADTFAYPMLDLQALS
PAALPAKAIDPKLMQSQRVVALSKRGNKMSVAISDPTNTQALDQIKFQTETSVEPVIVPHDALVRLLAELAKNSEQLMGE
LAGEEAEIQFAEEQEAALVEAPATDVEDAPIVRFLNKMLMDAVNMGASDLHFEPFEKFYRIRFRVDGVLVEHAQPPIAIK
DKLVSRIKVLAKLDISEKRVPQDGRMRLIVSPTKTVDLRISTLPTLFGEKTVMRILDPTQAQMGIDALGYDADQKALLLD
AISRPYGMVLVTGPTGSGKTVSLYTCLNILNKPGINISTAEDPAEINLPGVNQVNVNDKAGLTFPVALKSFLRQDPDIIM
VGEIRDLETADIAIKAAQTGHMVFSTLHTNDAPSTLTRLMNMGVAPFNIASSIVLITAQRLARRLCACKRPVDVSTDLLL
RAGFRPAQLDGSWKPYGPVGCERCNGTGYKGRVGIYQIMPITPAIEALILAHGNAMQIAAQSELEGVRSLRQSGLVKVKA
GLTSLEEVLGCTNE

Nucleotide


Download         Length: 1725 bp        

>NTDB_id=351543 E1742_RS21270 WP_134387125.1 4789722..4791446(-) (pilB) [Pseudoduganella plicata strain DSM 17505]
ATGGCAGCAGTCCAACCCACCACGGCGGCAGGCGCTCCCATGTCGGGCCTGGCGCGTGCCTTGATGCAGGCGGGGCGTCT
CACGCCGCCGCAGGCGGACGCGCTGCTGAAAAAATCCATCCTGGAAAAACTGCCGTTCATCGACGTGCTGCTGGCCAGCG
GTGCGATCGGCGGCCGCGAACTGGCCGTGTTCTGCGCCGACACCTTCGCCTATCCGATGCTGGATCTGCAGGCGCTCTCC
CCGGCCGCACTGCCCGCCAAGGCGATCGACCCGAAACTGATGCAGAGCCAGCGGGTGGTGGCGCTGTCGAAACGGGGCAA
CAAGATGTCCGTGGCGATATCGGACCCCACCAACACGCAGGCACTGGACCAGATCAAGTTCCAGACCGAAACGTCGGTGG
AACCCGTCATCGTGCCGCACGACGCGCTGGTGCGCCTGCTGGCGGAGCTGGCCAAGAACAGCGAACAGCTGATGGGCGAG
CTGGCCGGTGAAGAGGCAGAGATCCAGTTCGCCGAAGAACAGGAAGCGGCGCTGGTGGAGGCGCCGGCCACGGACGTCGA
GGATGCGCCGATCGTGCGCTTCCTGAACAAGATGTTGATGGACGCCGTCAACATGGGGGCGTCGGACCTGCACTTCGAGC
CGTTCGAGAAGTTCTACCGCATCCGCTTCCGTGTCGACGGTGTGCTGGTCGAGCATGCGCAGCCGCCTATCGCCATCAAG
GACAAGCTGGTGTCGCGCATCAAGGTGCTGGCGAAACTGGACATCTCGGAAAAACGCGTCCCGCAGGACGGGCGCATGCG
CCTGATCGTCTCGCCGACGAAAACGGTGGACCTGCGTATCTCCACGTTGCCCACGCTGTTCGGCGAAAAGACGGTGATGC
GTATCCTCGACCCCACGCAGGCGCAGATGGGCATCGATGCGCTGGGCTACGACGCGGACCAGAAGGCGCTGCTGCTCGAT
GCGATCTCGCGCCCGTACGGCATGGTGCTGGTGACGGGTCCGACAGGCTCCGGCAAGACGGTGTCGCTGTACACCTGCCT
GAACATCCTGAACAAGCCGGGCATCAATATCTCGACGGCGGAAGACCCGGCCGAGATCAACCTGCCCGGTGTGAACCAGG
TCAATGTCAACGACAAGGCGGGGCTGACGTTCCCCGTCGCGCTGAAGTCCTTCCTGCGCCAGGATCCGGACATCATCATG
GTCGGCGAGATCCGCGACCTGGAGACGGCGGACATCGCCATCAAGGCGGCGCAGACGGGCCACATGGTGTTTTCGACGCT
GCACACGAACGATGCGCCATCGACGTTGACGCGCCTCATGAACATGGGCGTGGCGCCGTTCAATATCGCATCGTCGATTG
TATTGATCACGGCGCAGCGCCTGGCGCGGCGGCTGTGCGCATGCAAGCGTCCCGTCGACGTCTCGACCGACCTGCTGCTG
CGGGCCGGCTTCCGGCCGGCGCAACTCGATGGCAGCTGGAAGCCGTACGGCCCGGTGGGCTGCGAACGGTGCAACGGCAC
CGGTTACAAGGGCCGCGTGGGCATCTACCAGATCATGCCGATCACGCCGGCAATCGAGGCGCTGATCCTGGCGCACGGTA
ATGCCATGCAGATCGCCGCGCAGTCCGAGCTTGAGGGCGTGCGCTCGCTGCGCCAGTCGGGCCTCGTGAAGGTCAAGGCG
GGTCTGACCAGCCTGGAAGAAGTGCTGGGCTGCACCAACGAGTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(197-464)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(50-155)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

53.752

99.826

0.537
  pilF Neisseria gonorrhoeae MS11

52.827

98.606

0.521
  pilB Acinetobacter baumannii D1279779

55.303

91.986

0.509
  pilB Legionella pneumophila strain ERS1305867

46.881

97.735

0.458
  pilB Vibrio cholerae strain A1552

45.455

97.735

0.444
  pilB Vibrio parahaemolyticus RIMD 2210633

44.779

98.432

0.441
  pilB Vibrio campbellii strain DS40M4

43.64

98.606

0.43
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.177

97.387

0.382
  pilF Thermus thermophilus HB27

37.766

98.258

0.371

Multiple sequence alignment