Detailed information NTG0350904

Bioinformatically predicted

Overview

• Name: pilB
• Type: Machinery gene
• Locus tag: E2H97_RS17570
• Genome accession: NZ_CP037951
• Coordinates: 3979679..3981385 (+)
• Length: 568 a.a.
• NCBI ID: WP_133408338.1
• Uniprot ID: -
• Organism: Parashewanella tropica strain MEBiC05444
• Function: power the assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3974679..3986385

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
E2H97_RS17555	nadC	3975850..3976743 (+)	894	WP_133408701.1	carboxylating nicotinate-nucleotide diphosphorylase	-
E2H97_RS17560	-	3976834..3978264 (-)	1431	WP_133408336.1	ankyrin repeat domain-containing protein	-
E2H97_RS19080	-	3979130..3979549 (+)	420	WP_178074608.1	prepilin-type N-terminal cleavage/methylation domain-containing protein	-
E2H97_RS17570	pilB	3979679..3981385 (+)	1707	WP_133408338.1	type IV-A pilus assembly ATPase PilB	Machinery gene
E2H97_RS17575	-	3981487..3981774 (+)	288	WP_133408339.1	GIY-YIG nuclease family protein	-
E2H97_RS17580	pilC	3981901..3983169 (+)	1269	WP_133408340.1	type II secretion system F family protein	Machinery gene
E2H97_RS17585	pilD	3983273..3984190 (+)	918	WP_133408341.1	A24 family peptidase	Machinery gene
E2H97_RS17590	coaE	3984187..3984792 (+)	606	WP_133408342.1	dephospho-CoA kinase	-
E2H97_RS17595	zapD	3984802..3985533 (+)	732	WP_133408343.1	cell division protein ZapD	-
E2H97_RS17600	yacG	3985607..3985819 (+)	213	WP_133408344.1	DNA gyrase inhibitor YacG	-
E2H97_RS17605	mutT	3985819..3986211 (+)	393	WP_133408345.1	8-oxo-dGTP diphosphatase MutT	-

Sequence

Protein

• Length: 568 a.a.
• Molecular weight: 63298.70 Da
• Isoelectric Point: 5.3618

>NTDB_id=350904 E2H97_RS17570 WP_133408338.1 3979679..3981385(+) (pilB) [Parashewanella tropica strain MEBiC05444]
MPQSGLHIAFSTLLIRQQLIDNKKLEQLIKLANKNKLTFIQALIHDGVLTAKEIVELGHQEYHTPKMDLAEFDISSIPET
FLNLKLIEQHRCLPLFEKKNRLYIATSDPTNFRALEDFQFNLGMHTEAVLVEDDKLSDVLERVLEDDLTSLDLSDMDEEA
LAGVEVAETERKDDDDNDGKDDAPIVIYINKILTDAIRKGASDLHFEPYEKRYRIRFRIDGVLQQISEPPISLASRIAAR
LKVMSKLDIAERRVPQDGRIKLRVSRKKSTDFRVSTLPTIWGEKIVMRILDSESAQLGIEKLGYEPTQQKMYTDALAQPQ
GMILVTGPTGSGKTVSLYTGLNILNTEERNISTAEDPVEINLEGVNQVHINTKAGLTFASALRSFLRQDPDIVMVGEIRD
LETAEIAIKAAQTGHLVLSTLHTNSAAETLTRLSNMGVAGYNIASSVTLIIAQRLARRLCEKCKAPEDVPVAELERLGFS
QKDIDSKFTVFKPVGCEKCTAGFKGRVGIYEVMKMSGELAKCIMEGGNSLELLEIAKKEGMDTLRQSGLSKVVQGISSIN
EINRVTSF

Nucleotide

• Length: 1707 bp

>NTDB_id=350904 E2H97_RS17570 WP_133408338.1 3979679..3981385(+) (pilB) [Parashewanella tropica strain MEBiC05444]
ATGCCTCAATCCGGTCTTCATATTGCCTTTTCTACCTTACTGATACGCCAACAGCTGATTGATAACAAAAAGCTCGAACA
GCTGATTAAGCTTGCTAACAAAAACAAACTCACCTTTATTCAAGCCTTAATTCACGATGGAGTGCTCACCGCCAAAGAGA
TTGTTGAGCTAGGGCATCAGGAATACCATACCCCTAAAATGGACTTGGCTGAGTTTGATATCAGCAGTATTCCCGAAACT
TTTTTAAATTTAAAGCTGATTGAACAACACCGATGTTTGCCGCTGTTTGAAAAGAAAAACCGTCTTTATATTGCGACGTC
CGACCCAACCAACTTCCGCGCTCTTGAGGACTTTCAGTTTAACCTTGGCATGCACACCGAAGCGGTATTGGTGGAAGACG
ATAAACTCAGTGACGTTTTAGAAAGAGTGCTTGAGGATGACTTAACCAGCCTAGATTTATCCGATATGGATGAAGAAGCC
CTTGCTGGTGTGGAAGTCGCGGAAACCGAACGTAAAGACGATGATGATAACGACGGCAAAGATGATGCCCCAATTGTTAT
CTACATTAATAAAATTTTGACCGATGCCATTAGAAAAGGCGCATCGGATTTACACTTTGAACCTTACGAAAAGCGTTACC
GTATTCGTTTTCGTATTGATGGTGTACTCCAACAAATTTCAGAGCCTCCGATTTCCCTCGCCAGCCGCATTGCCGCCCGT
TTAAAAGTGATGTCAAAACTGGACATTGCCGAGCGTCGTGTGCCGCAAGATGGTCGAATAAAATTACGCGTCAGCCGTAA
GAAGTCTACTGACTTTCGTGTCAGTACCCTGCCCACCATTTGGGGCGAAAAAATCGTAATGCGTATTTTGGACTCAGAAT
CGGCGCAACTGGGTATCGAAAAACTCGGCTATGAGCCAACTCAGCAAAAAATGTATACCGATGCCTTAGCGCAGCCCCAA
GGCATGATTTTGGTGACAGGCCCAACAGGCTCAGGTAAAACCGTTTCTTTGTATACGGGTCTTAATATTCTTAACACCGA
AGAGCGCAATATTTCCACCGCCGAAGATCCTGTGGAAATTAACCTTGAAGGCGTTAATCAAGTTCATATTAATACCAAAG
CAGGCTTAACTTTTGCTTCAGCACTTCGCTCTTTCTTACGTCAAGATCCCGATATTGTTATGGTGGGTGAGATCCGTGAT
TTAGAAACTGCTGAAATCGCCATTAAAGCGGCACAAACAGGTCACTTGGTATTATCGACCTTACATACCAACTCAGCGGC
TGAAACTCTAACCCGTTTATCCAATATGGGCGTTGCTGGCTATAACATTGCCAGCTCGGTTACCTTAATCATCGCCCAGC
GTCTTGCCAGACGCTTATGTGAGAAATGCAAAGCCCCCGAAGACGTACCCGTTGCAGAATTAGAGCGCCTAGGGTTCAGT
CAAAAAGACATCGACAGCAAATTTACCGTCTTCAAACCCGTCGGCTGCGAAAAATGTACCGCAGGCTTTAAAGGCCGAGT
CGGTATTTACGAAGTGATGAAAATGTCAGGAGAACTGGCAAAATGCATTATGGAAGGTGGAAATTCGCTTGAGCTTCTAG
AAATCGCCAAAAAAGAAGGCATGGATACTCTACGTCAATCAGGCTTAAGTAAAGTCGTACAAGGGATCAGCAGCATTAAC
GAAATCAATCGAGTAACGAGTTTTTAA

Domains

Predicted by InterproScan.

• Type II secretion system (T2SS), protein E, N-terminal domain (MshEN) (43-146)

• Type II/IV secretion system protein (T2SSE) (191-458)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilB	Acinetobacter baylyi ADP1	57.295	98.944	0.567
pilB	Acinetobacter baumannii D1279779	55.439	100	0.556
pilB	Legionella pneumophila strain ERS1305867	56.128	99.12	0.556
pilB	Vibrio parahaemolyticus RIMD 2210633	50.441	99.824	0.504
pilB	Vibrio cholerae strain A1552	50.536	98.592	0.498
pilB	Vibrio campbellii strain DS40M4	50	99.296	0.496
pilF	Neisseria gonorrhoeae MS11	46.549	99.472	0.463
pilB/pilB1	Synechocystis sp. PCC 6803	38.973	92.606	0.361