Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   E0Z08_RS01715 Genome accession   NZ_CP037424
Coordinates   367523..369235 (-) Length   570 a.a.
NCBI ID   WP_114392504.1    Uniprot ID   -
Organism   Acinetobacter johnsonii strain M19     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 362523..374235
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  E0Z08_RS01685 (E0Z08_01685) - 362619..362942 (+) 324 WP_004692718.1 pyrimidine/purine nucleoside phosphorylase -
  E0Z08_RS01690 (E0Z08_01690) rlmB 363085..363834 (+) 750 WP_004692717.1 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB -
  E0Z08_RS01695 (E0Z08_01695) - 363867..364784 (+) 918 WP_058868791.1 DMT family transporter -
  E0Z08_RS01700 (E0Z08_01700) coaE 364798..365397 (-) 600 WP_058868792.1 dephospho-CoA kinase -
  E0Z08_RS01705 (E0Z08_01705) pilD 365406..366266 (-) 861 WP_058868793.1 prepilin peptidase Machinery gene
  E0Z08_RS01710 (E0Z08_01710) pilC 366266..367492 (-) 1227 WP_131229878.1 type II secretion system F family protein Machinery gene
  E0Z08_RS01715 (E0Z08_01715) pilB 367523..369235 (-) 1713 WP_114392504.1 type IV-A pilus assembly ATPase PilB Machinery gene
  E0Z08_RS01720 (E0Z08_01720) tpiA 369515..370306 (+) 792 WP_058868795.1 triose-phosphate isomerase -
  E0Z08_RS01725 (E0Z08_01725) secG 370320..370649 (+) 330 WP_058868796.1 preprotein translocase subunit SecG -
  E0Z08_RS01750 (E0Z08_01750) rimP 371459..371983 (+) 525 WP_058868797.1 ribosome maturation factor RimP -
  E0Z08_RS01755 (E0Z08_01755) nusA 372023..373507 (+) 1485 WP_004692706.1 transcription termination factor NusA -

Sequence

Protein


Download         Length: 570 a.a.        Molecular weight: 63617.92 Da        Isoelectric Point: 4.9493

>NTDB_id=349679 E0Z08_RS01715 WP_114392504.1 367523..369235(-) (pilB) [Acinetobacter johnsonii strain M19]
MSVSNGLPKFTGFIRRLVEDGRVTAENMQQAMLSAKKAQQDIVPYLIENYKLSPQMIAETISLEFGEPVFDLSVYDSVQI
LRDLVEDKLITKHRVLPIYRRGNILYIATSNPTNMDAMDAIRFNSKMNIEAIIVEHPQLEKLIEQNFAQADSFEFSDEDI
DLDLEQDHQNSNDEEDDSPQGDEAPIVKYINKLLIDAIRMGASDLHFEPYEKSYRVRYRVDGVLRHIASPPLQLATRLAS
RLKVMSQMDISEKRVPQDGRIKLKLSKSKAIDFRVNSLPTLFGEKLVLRILDPSSAMLGIDALGYEPDQKDLFMQALDKP
QGMLLITGPTGSGKTVSLYTGLNILNKEDTNISTAEDPVEINLEGINQVNVNNKVGLTFSAALKSFLRQDPDIVMVGEIR
DLETAEIAIKAAQTGHMVMSTLHTNSAPETLTRLRNMGVPSFNIATSVNLVIAQRLARRLCSQCKAAVDIPEQSLLEMGF
TSTDIKDPDFKVYQPVGCTECREGYKGRVGIYEVMKVTPEISKIIMEDGNALQIAEASAKLGFNNLRRSGLLKVMQGVTS
LQEVNRVTSE

Nucleotide


Download         Length: 1713 bp        

>NTDB_id=349679 E0Z08_RS01715 WP_114392504.1 367523..369235(-) (pilB) [Acinetobacter johnsonii strain M19]
ATGTCAGTATCAAATGGTTTGCCTAAGTTCACAGGCTTCATTCGACGCTTAGTCGAAGACGGACGCGTGACTGCTGAAAA
TATGCAGCAGGCAATGCTCTCTGCAAAAAAGGCTCAGCAAGACATCGTTCCTTATCTAATTGAAAATTATAAACTCTCCC
CACAAATGATCGCTGAGACGATTTCTTTGGAGTTTGGCGAGCCTGTTTTTGACTTAAGTGTCTATGATTCGGTACAAATT
CTACGGGATCTGGTCGAAGACAAACTCATCACCAAGCATCGTGTGTTGCCGATTTACCGTCGTGGCAATATCCTCTATAT
CGCCACCAGCAATCCAACCAACATGGATGCAATGGACGCGATTCGTTTCAATTCTAAAATGAACATTGAAGCGATTATTG
TTGAACATCCTCAGCTTGAAAAACTAATCGAACAAAATTTCGCGCAAGCCGATAGCTTTGAATTTTCTGATGAAGATATT
GATTTAGACTTAGAGCAAGACCATCAGAACTCCAATGACGAAGAAGATGACTCGCCTCAAGGGGATGAAGCGCCGATCGT
CAAATACATCAATAAATTACTGATCGATGCGATTCGTATGGGCGCATCAGACTTGCACTTTGAACCTTATGAAAAATCTT
ACCGTGTCCGTTATCGAGTAGATGGGGTTCTGCGTCATATTGCCAGTCCTCCACTACAACTGGCGACTCGTCTTGCCTCA
CGTTTAAAAGTTATGTCACAAATGGACATCTCTGAAAAGCGCGTCCCGCAAGATGGCCGGATTAAATTAAAGCTGTCGAA
AAGTAAAGCCATTGACTTTCGTGTCAACTCACTTCCAACCTTGTTTGGTGAAAAACTGGTTCTGCGTATTCTCGATCCAT
CCAGTGCTATGCTGGGGATTGATGCTTTAGGTTATGAACCAGATCAAAAAGACCTATTTATGCAGGCTTTAGACAAACCG
CAAGGCATGTTGTTAATTACTGGACCGACAGGTTCAGGTAAAACGGTATCGCTGTATACAGGTTTGAATATTCTAAATAA
AGAAGACACCAATATCTCAACTGCAGAAGACCCCGTTGAGATTAACTTAGAAGGCATTAATCAGGTTAACGTGAACAATA
AAGTCGGTCTTACCTTCTCTGCTGCGCTGAAGTCATTCTTACGTCAAGATCCTGATATTGTAATGGTGGGTGAGATTCGT
GACCTTGAAACGGCTGAAATCGCGATTAAAGCCGCACAGACAGGCCACATGGTCATGTCGACACTCCACACCAATAGTGC
ACCTGAAACTCTGACACGCTTACGCAATATGGGGGTTCCATCTTTTAACATTGCGACATCGGTCAATTTGGTAATTGCAC
AGCGTTTGGCACGCCGTTTATGTAGCCAATGCAAAGCCGCTGTCGATATTCCTGAACAAAGCTTGCTCGAAATGGGCTTT
ACTTCAACAGATATCAAAGATCCAGACTTCAAAGTTTATCAACCGGTGGGATGTACTGAGTGCCGTGAAGGCTATAAAGG
ACGTGTTGGTATTTATGAAGTCATGAAAGTCACACCAGAAATTTCAAAAATTATTATGGAAGATGGCAATGCCTTACAGA
TTGCGGAAGCATCTGCAAAATTAGGATTTAACAATTTACGTCGTTCAGGTTTACTCAAGGTTATGCAGGGGGTCACTTCC
TTACAAGAAGTGAATCGTGTAACCAGCGAATAG

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(46-150)

Type II/IV secretion system protein (T2SSE)

(192-459)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

81.404

100

0.814
  pilB Acinetobacter baylyi ADP1

76.842

100

0.768
  pilB Legionella pneumophila strain ERS1305867

53.933

93.684

0.505
  pilF Neisseria gonorrhoeae MS11

50

98.596

0.493
  pilB Vibrio cholerae strain A1552

48.677

99.474

0.484
  pilB Vibrio campbellii strain DS40M4

47.67

97.895

0.467
  pilB Vibrio parahaemolyticus RIMD 2210633

50.677

90.702

0.46
  pilB/pilB1 Synechocystis sp. PCC 6803

34.572

100

0.375
  pilF Thermus thermophilus HB27

36.602

100

0.367

Multiple sequence alignment