Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   EZZ81_RS24770 Genome accession   NZ_CP036495
Coordinates   5575139..5577238 (-) Length   699 a.a.
NCBI ID   WP_029243167.1    Uniprot ID   -
Organism   Pseudomonas viridiflava strain Psy35     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5570139..5582238
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EZZ81_RS24755 (EZZ81_25130) - 5571761..5573377 (-) 1617 WP_029243165.1 SPOR domain-containing protein -
  EZZ81_RS24760 (EZZ81_25135) aroB 5573387..5574490 (-) 1104 WP_029243166.1 3-dehydroquinate synthase -
  EZZ81_RS24765 (EZZ81_25140) aroK 5574616..5575134 (-) 519 WP_004882714.1 shikimate kinase AroK -
  EZZ81_RS24770 (EZZ81_25145) pilQ 5575139..5577238 (-) 2100 WP_029243167.1 type IV pilus secretin PilQ Machinery gene
  EZZ81_RS24775 (EZZ81_25150) - 5577286..5577813 (-) 528 WP_029243168.1 pilus assembly protein PilP -
  EZZ81_RS24780 (EZZ81_25155) pilO 5577810..5578433 (-) 624 WP_004882717.1 type 4a pilus biogenesis protein PilO -
  EZZ81_RS24785 (EZZ81_25160) - 5578430..5579008 (-) 579 WP_025993896.1 PilN domain-containing protein -
  EZZ81_RS24790 (EZZ81_25165) pilM 5579008..5580072 (-) 1065 WP_004882719.1 pilus assembly protein PilM Machinery gene

Sequence

Protein


Download         Length: 699 a.a.        Molecular weight: 75331.72 Da        Isoelectric Point: 4.9910

>NTDB_id=349158 EZZ81_RS24770 WP_029243167.1 5575139..5577238(-) (pilQ) [Pseudomonas viridiflava strain Psy35]
MTRILSIIGVSLWIAMMSPVLQAANLKTLDVAALPGDRIELKLSFDAPVPAPRGYTTEQPARIALDLPGVTSQLATKSRD
LGAGNARSVVVVQAQDRTRVIINLTTLSPYSSRVDGNNLFVVIGQGAAAAQASQPTTAKGAARVAVPPPTPASARPYVPA
GVRAIRNVDFQRGELGEGNVVIDLSNASIAADIQEQGGKIRVDFAKTQLPDPLRVRLDVKDFATPVQFVSATASGDKASI
SIEPSGAFDYSAFQTENKLTISVRPLTSEDVDRRAADKPVYTGEKLSLNFQDIDVRSVLQLIADFTNLNLVASDTVQGGI
TLRLQNVPWDQALDLVLKTKGLDKRKVGSVLLVAPADEIAARERQELESLKQIAELAPLRRELLQVNYAKAADIAKLFQS
VTSAESKADERGSITVDERTNNIIAYQTQDRLDELRRIVSQLDIPVRQVMIEARIVEANVDYDKQIGVRWGGRTDRSRKW
SVGGLDDNGDEAGNTGNDLTANIPFVDLGAPDATAGVGIGFLTNNALLDLELSAMEKTGNGEIVSQPKVVTSDKETAKIL
KGTEIPYQESSSSGATTVSFKEASLSLEVTPQITPDNRIIMEVKVTKDEPDYLNAVLGVPPIKKNEVNAKVLISDGETIV
IGGVFSNTQSKVVEKVPFLGDVPYLGRLFRRDVVAEAKSELLVFLTPRIMNNQAIAVSR

Nucleotide


Download         Length: 2100 bp        

>NTDB_id=349158 EZZ81_RS24770 WP_029243167.1 5575139..5577238(-) (pilQ) [Pseudomonas viridiflava strain Psy35]
ATGACCCGGATTCTCTCGATTATCGGCGTTTCGTTATGGATAGCGATGATGTCGCCGGTTCTCCAGGCCGCCAATCTCAA
GACGCTCGACGTCGCAGCCCTGCCGGGTGATCGAATCGAGCTCAAGCTTTCCTTCGATGCACCGGTGCCCGCGCCACGCG
GCTATACCACCGAGCAGCCTGCGCGCATTGCGCTGGACCTGCCGGGCGTGACCAGCCAGCTGGCGACCAAGAGCCGTGAT
CTGGGCGCAGGCAATGCCCGCAGTGTGGTGGTTGTGCAGGCGCAGGACCGCACGCGAGTCATCATCAACCTGACGACGCT
TTCGCCGTACAGCTCACGCGTCGATGGCAACAACCTGTTCGTGGTGATCGGTCAGGGTGCGGCGGCGGCACAGGCTTCGC
AGCCGACCACCGCCAAGGGCGCTGCACGGGTCGCCGTGCCGCCACCCACACCGGCATCGGCGCGGCCCTATGTGCCTGCG
GGCGTCAGAGCCATTCGCAATGTCGACTTCCAGCGCGGTGAGCTGGGCGAAGGCAACGTGGTCATCGACCTGAGCAATGC
CAGCATCGCTGCAGATATTCAGGAGCAGGGCGGCAAAATCCGTGTCGACTTCGCCAAGACGCAATTGCCCGATCCGTTGC
GCGTGCGTCTGGACGTGAAAGACTTTGCCACACCCGTGCAATTCGTCAGCGCCACGGCGTCCGGCGACAAGGCCAGCATT
TCCATCGAGCCATCGGGTGCGTTCGATTATTCGGCGTTCCAGACCGAAAACAAGCTGACCATCAGCGTGCGCCCATTGAC
CAGTGAGGACGTGGATCGTCGCGCTGCTGACAAGCCGGTCTACACCGGCGAGAAGCTTTCGCTGAATTTTCAGGACATCG
ATGTGCGTTCGGTACTGCAACTGATCGCCGATTTCACTAACCTCAATCTGGTCGCCAGTGACACGGTTCAGGGCGGCATC
ACCCTGCGTCTGCAGAACGTGCCGTGGGATCAGGCGCTGGACCTGGTGCTCAAGACCAAAGGGCTGGACAAGCGCAAGGT
CGGCAGCGTGTTGCTGGTCGCCCCGGCTGACGAAATCGCCGCGCGTGAGCGTCAGGAGCTGGAGTCGCTCAAGCAGATTG
CCGAACTGGCCCCGTTGCGTCGTGAGCTGTTGCAGGTCAATTACGCCAAGGCAGCGGACATCGCCAAGCTGTTCCAGTCG
GTCACCAGCGCCGAGTCCAAGGCGGACGAGCGTGGTTCCATTACTGTGGATGAGCGCACCAACAACATCATCGCCTACCA
GACTCAGGATCGACTCGACGAGCTGCGCCGTATCGTCTCGCAGCTGGATATTCCGGTACGTCAGGTGATGATCGAGGCGC
GGATAGTTGAAGCCAACGTCGACTATGACAAGCAGATCGGCGTGCGCTGGGGCGGGCGAACCGACCGGTCGCGCAAGTGG
AGCGTCGGCGGTCTCGACGATAACGGCGATGAAGCAGGCAATACGGGCAACGATCTGACGGCCAACATTCCGTTTGTGGA
TCTGGGTGCTCCAGATGCCACGGCGGGTGTCGGGATCGGTTTCCTGACCAACAATGCACTGCTGGACCTTGAGCTGAGTG
CTATGGAGAAGACCGGCAACGGTGAAATCGTCTCCCAGCCCAAGGTCGTCACCTCCGACAAGGAGACTGCCAAGATCCTC
AAGGGCACGGAGATTCCGTATCAGGAATCCAGCTCCAGCGGTGCCACGACAGTGAGCTTCAAGGAGGCGTCCCTGTCGCT
TGAGGTCACGCCACAGATCACGCCGGACAACCGCATCATCATGGAGGTCAAGGTTACCAAGGACGAGCCTGATTACCTCA
ATGCGGTGCTGGGCGTGCCTCCTATCAAGAAGAACGAGGTCAACGCCAAGGTGCTGATTTCCGACGGCGAGACCATCGTG
ATCGGCGGGGTGTTCTCCAATACGCAAAGTAAAGTTGTAGAGAAAGTGCCATTTCTCGGTGATGTGCCGTATCTTGGCCG
CCTTTTCAGGCGCGACGTGGTGGCAGAGGCGAAATCCGAGCTGTTGGTATTTCTGACACCGCGTATCATGAACAACCAGG
CGATTGCTGTGAGTCGTTGA

Domains

Predicted by InterproScan.

Secretin and TonB N terminus short domain (STN)

(309-355)

Bacterial type II/III secretion system short domain (Secretin_N)

(383-448)

AMIN domain (AMIN)

(37-122)

Bacterial type II and III secretion system protein (Secretin)

(534-690)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

72.399

100

0.747
  comQ Acinetobacter baylyi ADP1

40.656

100

0.408
  pilQ Acinetobacter baumannii strain A118

37.756

100

0.395
  pilQ Acinetobacter baumannii D1279779

37.756

100

0.395
  pilQ Legionella pneumophila strain Lp02

38.067

97.711

0.372
  pilQ Legionella pneumophila strain ERS1305867

38.067

97.711

0.372

Multiple sequence alignment