Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   EZZ81_RS24790 Genome accession   NZ_CP036495
Coordinates   5579008..5580072 (-) Length   354 a.a.
NCBI ID   WP_004882719.1    Uniprot ID   A0A0D0MR84
Organism   Pseudomonas viridiflava strain Psy35     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5574008..5585072
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EZZ81_RS24765 (EZZ81_25140) aroK 5574616..5575134 (-) 519 WP_004882714.1 shikimate kinase AroK -
  EZZ81_RS24770 (EZZ81_25145) pilQ 5575139..5577238 (-) 2100 WP_029243167.1 type IV pilus secretin PilQ Machinery gene
  EZZ81_RS24775 (EZZ81_25150) - 5577286..5577813 (-) 528 WP_029243168.1 pilus assembly protein PilP -
  EZZ81_RS24780 (EZZ81_25155) pilO 5577810..5578433 (-) 624 WP_004882717.1 type 4a pilus biogenesis protein PilO -
  EZZ81_RS24785 (EZZ81_25160) - 5578430..5579008 (-) 579 WP_025993896.1 PilN domain-containing protein -
  EZZ81_RS24790 (EZZ81_25165) pilM 5579008..5580072 (-) 1065 WP_004882719.1 pilus assembly protein PilM Machinery gene
  EZZ81_RS24795 (EZZ81_25170) - 5580282..5582723 (+) 2442 WP_406656099.1 penicillin-binding protein 1A -
  EZZ81_RS24800 (EZZ81_25175) - 5583071..5583526 (+) 456 WP_029243170.1 YkgJ family cysteine cluster protein -

Sequence

Protein


Download         Length: 354 a.a.        Molecular weight: 38031.45 Da        Isoelectric Point: 4.7090

>NTDB_id=349159 EZZ81_RS24790 WP_004882719.1 5579008..5580072(-) (pilM) [Pseudomonas viridiflava strain Psy35]
MFELFGKKANTLLGIDISSTSVKLLELSRSGTRYKVESYAVEPLPANAVVEKNIAELEGVGQALSRVVGKAKTSARIVAV
AVAGSAVITKTIEMDAGLSDDDMENQLKLEADQYIPYPLEEVAIDFEVQGYSLRNPERVEVLLAACRKENVEVREAALAL
AGLTAKVVDVEAYALERSFGLLAAQLGNGHDELTVAVVDIGATMTTLSVLHHGRIIYTREQLFGGRQLTEEIQRRYGLSM
EEAGLAKKQGGLPDDYVSEVLEPFKDALVQQVSRSLQFFFAAGQYNSVDHIMLAGGTASISGLEHLIQRRLGTPTQVANP
FADMALSSKVNAGALASDAPALMIACGLALRSFD

Nucleotide


Download         Length: 1065 bp        

>NTDB_id=349159 EZZ81_RS24790 WP_004882719.1 5579008..5580072(-) (pilM) [Pseudomonas viridiflava strain Psy35]
GTGTTCGAACTCTTCGGTAAGAAGGCCAACACCCTTCTAGGGATCGATATTAGTTCCACCTCGGTCAAGCTCCTTGAATT
GAGTCGTTCCGGAACCCGCTACAAAGTCGAGTCTTACGCTGTTGAGCCGTTGCCGGCCAATGCCGTCGTCGAGAAGAACA
TTGCCGAACTCGAAGGGGTTGGTCAGGCGTTGTCACGGGTCGTTGGCAAGGCCAAGACCAGCGCCAGAATAGTGGCGGTC
GCTGTCGCAGGCTCCGCAGTCATCACCAAGACGATCGAAATGGATGCCGGTCTCTCGGACGACGACATGGAAAATCAGCT
CAAGCTCGAGGCTGACCAGTACATCCCCTATCCCCTTGAAGAAGTGGCCATCGATTTCGAAGTGCAGGGCTATTCGCTGC
GCAACCCCGAACGCGTGGAAGTCCTTCTGGCTGCCTGCCGCAAGGAGAACGTCGAGGTGCGCGAGGCCGCGCTGGCGCTG
GCGGGCCTAACCGCAAAAGTCGTCGACGTCGAAGCCTATGCCCTTGAGCGTTCGTTCGGGCTGCTGGCTGCGCAACTGGG
TAACGGTCACGATGAGCTGACCGTGGCGGTCGTCGACATCGGCGCCACCATGACCACACTGAGCGTCCTGCACCATGGCC
GGATCATTTATACCCGTGAGCAACTGTTCGGCGGGCGCCAACTGACCGAAGAAATCCAGCGCCGCTATGGCCTGTCGATG
GAGGAGGCCGGGCTTGCCAAGAAACAGGGCGGGCTGCCGGACGACTATGTCAGTGAAGTGCTGGAACCCTTCAAGGATGC
GTTGGTGCAGCAGGTCTCGCGCTCACTTCAGTTCTTCTTTGCGGCAGGCCAGTACAACTCTGTGGATCACATCATGCTCG
CTGGCGGTACGGCTTCCATTTCCGGCCTGGAGCATCTGATTCAGCGTCGACTCGGCACGCCGACCCAGGTGGCCAATCCG
TTTGCCGACATGGCCCTCAGTTCCAAGGTCAACGCCGGAGCGCTGGCCAGTGATGCGCCCGCGCTGATGATCGCGTGCGG
TCTTGCTTTGAGGAGCTTCGACTGA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(14-353)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0D0MR84

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Acinetobacter baumannii D1279779

56.78

100

0.568
  comM Acinetobacter nosocomialis M2

56.78

100

0.568
  comM Acinetobacter baylyi ADP1

55.085

100

0.551
  pilM Legionella pneumophila strain ERS1305867

47.887

100

0.48

Multiple sequence alignment