Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   MSIP34_RS02985 Genome accession   NC_012969
Coordinates   612367..614088 (-) Length   573 a.a.
NCBI ID   WP_015829464.1    Uniprot ID   C6X9L5
Organism   Methylovorus glucosotrophus SIP3-4     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 607367..619088
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MSIP34_RS02965 (Msip34_0583) - 607945..609117 (+) 1173 WP_015829462.1 FAD-dependent oxidoreductase -
  MSIP34_RS02970 (Msip34_0584) - 609114..610274 (+) 1161 WP_015829463.1 FAD-dependent monooxygenase -
  MSIP34_RS02975 (Msip34_0585) - 610297..611019 (+) 723 WP_013441415.1 DsbC family protein -
  MSIP34_RS02980 (Msip34_0586) pilC 611123..612334 (-) 1212 WP_013441416.1 type II secretion system F family protein Machinery gene
  MSIP34_RS02985 (Msip34_0587) pilB 612367..614088 (-) 1722 WP_015829464.1 type IV-A pilus assembly ATPase PilB Machinery gene
  MSIP34_RS02990 (Msip34_0588) - 614506..614709 (+) 204 WP_013441419.1 cold-shock protein -
  MSIP34_RS02995 (Msip34_0589) icd 614825..616063 (-) 1239 WP_015829465.1 NADP-dependent isocitrate dehydrogenase -
  MSIP34_RS03000 (Msip34_0590) - 616086..616622 (+) 537 WP_013441421.1 pseudouridine synthase -
  MSIP34_RS03005 (Msip34_0591) - 616711..617418 (+) 708 WP_015829466.1 type 1 glutamine amidotransferase -
  MSIP34_RS03010 (Msip34_0592) aroC 617488..618573 (+) 1086 WP_015829467.1 chorismate synthase -

Sequence

Protein


Download         Length: 573 a.a.        Molecular weight: 62264.60 Da        Isoelectric Point: 4.8492

>NTDB_id=34789 MSIP34_RS02985 WP_015829464.1 612367..614088(-) (pilB) [Methylovorus glucosotrophus SIP3-4]
MAATTLPSKLGGLARMLVQEQLLSEEDAASVQASATAQRIPFVSQLVQSKKLPALKIAETAANAFGFPLFDLDALNPDYI
PAKTIDTKLMASSRVMALQSRGNSLFVALSDPTNLHALDDVQFQVGMALSPVVVEEDKLSKWIARLVEASDTSMQSLSMS
DEFSLDMESGNEPVEQEVSSAEIDDAPVVKYIQKILLDAINMGASDLHFEPYEKYYRIRYRVDGVLQEIAQPPLAIKEKM
ASRIKVISNLDISEKRVPQDGRMKLVLSKTRTIDFRVSTLPMINGEKIVMRILDPSSATLGIEALGYEPDQKEALLSAVS
RPYGLVLVTGPTGSGKTVSLYTCLNILNSPGVNISTAEDPAEIPLPGINQVNVNDKQGLTFAAALKAFLRQDPDIIMIGE
IRDLETADMAIKAASTGHMVLSTLHTNDAPSTLTRLMNMGVAPFNIASAVSLITAQRLARRLCPHCREPLDLPKEALLSV
GFTEADLDGSWQLYGPREGGCEMCNGGYKGRVGIYQVMPVSDEMSRIIMKNGTAHDIADQAKREGVRDLRQSGLMKVKQG
LTSIAEVEAVTNE

Nucleotide


Download         Length: 1722 bp        

>NTDB_id=34789 MSIP34_RS02985 WP_015829464.1 612367..614088(-) (pilB) [Methylovorus glucosotrophus SIP3-4]
ATGGCAGCCACCACCCTTCCCAGTAAGTTAGGCGGGCTTGCACGCATGCTGGTGCAAGAGCAACTGCTATCGGAAGAAGA
CGCGGCCAGTGTACAGGCCAGTGCAACTGCACAACGCATCCCCTTTGTCAGCCAGCTGGTACAAAGCAAGAAACTGCCGG
CACTGAAAATTGCCGAGACGGCAGCCAATGCCTTCGGCTTCCCCTTGTTTGATCTTGATGCTTTAAATCCCGATTACATT
CCGGCCAAAACGATAGACACCAAGCTCATGGCGAGCAGCCGTGTCATGGCATTGCAAAGCCGCGGCAATTCGCTGTTTGT
CGCCCTGTCCGACCCCACCAATCTGCATGCCCTGGATGATGTGCAGTTTCAGGTGGGCATGGCGCTTTCCCCTGTGGTGG
TCGAAGAAGACAAACTGAGCAAGTGGATTGCCAGACTGGTGGAAGCGAGCGATACCAGCATGCAATCGCTCAGCATGAGC
GATGAATTTAGCCTGGACATGGAATCCGGCAATGAGCCGGTGGAGCAAGAAGTCAGCAGTGCGGAAATTGACGATGCTCC
GGTGGTGAAATACATCCAGAAGATACTGCTGGATGCGATCAACATGGGCGCCTCTGACCTGCATTTCGAACCGTATGAAA
AGTATTACCGCATTCGCTATCGGGTAGATGGTGTGTTGCAGGAAATCGCCCAGCCACCGCTGGCCATCAAGGAAAAAATG
GCCTCCCGCATCAAGGTGATCTCCAATCTGGATATCTCGGAAAAGCGTGTTCCGCAGGATGGCCGCATGAAACTGGTGCT
GTCCAAAACCCGGACCATCGACTTCCGCGTCAGCACCCTGCCCATGATCAATGGCGAAAAAATCGTCATGCGGATTCTGG
ACCCGAGCAGCGCCACGCTGGGCATTGAGGCATTGGGGTATGAGCCAGACCAGAAAGAGGCACTGCTCAGTGCCGTCAGC
CGCCCATATGGATTGGTGCTGGTCACCGGGCCAACTGGCTCAGGCAAAACCGTGTCCCTCTATACCTGCCTCAATATTCT
CAATAGCCCAGGCGTCAATATTTCCACGGCGGAAGACCCGGCGGAAATACCCTTGCCTGGCATCAACCAGGTGAATGTGA
ATGACAAGCAGGGCCTGACCTTTGCCGCCGCCTTGAAAGCATTTTTGCGGCAGGATCCCGATATCATCATGATCGGGGAA
ATTCGCGATCTGGAAACGGCCGACATGGCGATCAAGGCCGCCTCGACCGGGCACATGGTGCTGTCCACCCTGCATACCAA
CGATGCGCCATCGACCCTGACCCGCCTGATGAATATGGGCGTGGCGCCTTTCAATATTGCCTCGGCTGTTTCGCTGATTA
CAGCCCAGCGCCTGGCACGACGCTTGTGCCCGCATTGCAGAGAGCCACTCGACCTGCCAAAAGAAGCGCTGCTGAGTGTG
GGATTCACCGAAGCCGATCTGGATGGAAGCTGGCAACTGTATGGGCCGCGCGAGGGTGGCTGCGAGATGTGCAATGGCGG
CTACAAAGGTCGGGTGGGTATTTACCAGGTCATGCCCGTTTCGGATGAAATGAGCCGTATCATCATGAAAAACGGCACCG
CGCATGATATTGCGGACCAGGCCAAGCGCGAAGGCGTACGTGACCTTCGCCAGTCAGGCCTCATGAAAGTCAAACAGGGT
CTCACTTCCATCGCAGAGGTTGAGGCAGTGACCAATGAATAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(17-150)

Type II/IV secretion system protein (T2SSE)

(194-461)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB C6X9L5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

55.286

100

0.557
  pilB Acinetobacter baylyi ADP1

53.646

100

0.539
  pilF Neisseria gonorrhoeae MS11

52.972

99.825

0.529
  pilB Legionella pneumophila strain ERS1305867

51.646

100

0.52
  pilB Vibrio cholerae strain A1552

46.208

98.953

0.457
  pilB Vibrio campbellii strain DS40M4

44.602

98.604

0.44
  pilB Vibrio parahaemolyticus RIMD 2210633

44.484

98.08

0.436
  pilB/pilB1 Synechocystis sp. PCC 6803

38.047

100

0.394
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.477

92.147

0.382
  pilF Thermus thermophilus HB27

40.187

93.368

0.375

Multiple sequence alignment