Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   MSIP34_RS02980 Genome accession   NC_012969
Coordinates   611123..612334 (-) Length   403 a.a.
NCBI ID   WP_013441416.1    Uniprot ID   C6X9L4
Organism   Methylovorus glucosotrophus SIP3-4     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 606123..617334
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MSIP34_RS02960 (Msip34_0582) - 606644..607948 (+) 1305 WP_015829461.1 aminopeptidase P N-terminal domain-containing protein -
  MSIP34_RS02965 (Msip34_0583) - 607945..609117 (+) 1173 WP_015829462.1 FAD-dependent oxidoreductase -
  MSIP34_RS02970 (Msip34_0584) - 609114..610274 (+) 1161 WP_015829463.1 FAD-dependent monooxygenase -
  MSIP34_RS02975 (Msip34_0585) - 610297..611019 (+) 723 WP_013441415.1 DsbC family protein -
  MSIP34_RS02980 (Msip34_0586) pilC 611123..612334 (-) 1212 WP_013441416.1 type II secretion system F family protein Machinery gene
  MSIP34_RS02985 (Msip34_0587) pilB 612367..614088 (-) 1722 WP_015829464.1 type IV-A pilus assembly ATPase PilB Machinery gene
  MSIP34_RS02990 (Msip34_0588) - 614506..614709 (+) 204 WP_013441419.1 cold-shock protein -
  MSIP34_RS02995 (Msip34_0589) icd 614825..616063 (-) 1239 WP_015829465.1 NADP-dependent isocitrate dehydrogenase -
  MSIP34_RS03000 (Msip34_0590) - 616086..616622 (+) 537 WP_013441421.1 pseudouridine synthase -

Sequence

Protein


Download         Length: 403 a.a.        Molecular weight: 43836.64 Da        Isoelectric Point: 9.5190

>NTDB_id=34788 MSIP34_RS02980 WP_013441416.1 611123..612334(-) (pilC) [Methylovorus glucosotrophus SIP3-4]
MAAQASKEVIYAWEGTDKKGKKIKGEVKASGEAFVNATLRRQGITVTKIKKQSGLKSGGKVKEKDVTLFTRQLATMMKAG
VPLLQAFEIVGKGHNNPAVSKLLGDIKSDVETGSSLSQAFRKYPLYFDALYCNLVGAGEQAGILDSLLDRLATYKEKILA
IKSKIKSALFYPVSIIVVAFIITAVIMIFVIPAFKELFSSFGADLPAPTLIVMAISDFFVEYWWAIFGAIGGGVWFFFYS
WKRSIKMQATMDRLVLKIPVFGELIRKATIARFARTLSTMFAAGVPLVEALDSVAGASGNRVYYEATKQIQSEISTGTSL
TVAMQNVEVFPNMVLQMVAIGEESGALDSMLSKVADFYEAEVDDAVEALSSLMEPIIMVVLGTLIGGLVIAMYLPIFKMG
QVV

Nucleotide


Download         Length: 1212 bp        

>NTDB_id=34788 MSIP34_RS02980 WP_013441416.1 611123..612334(-) (pilC) [Methylovorus glucosotrophus SIP3-4]
ATGGCCGCACAAGCTAGCAAGGAAGTGATCTACGCCTGGGAAGGCACGGACAAAAAAGGCAAAAAAATCAAAGGCGAAGT
CAAAGCCAGTGGCGAAGCCTTCGTCAATGCCACCTTGCGTCGCCAGGGTATTACCGTTACCAAGATCAAAAAGCAGAGCG
GCCTCAAGTCTGGCGGCAAGGTCAAGGAAAAGGACGTCACGCTATTTACGCGTCAACTGGCCACCATGATGAAAGCCGGC
GTGCCTCTACTTCAAGCATTTGAGATTGTCGGCAAAGGGCATAACAATCCCGCGGTTTCCAAACTGCTGGGCGATATCAA
ATCCGACGTGGAAACTGGTAGCAGCCTCAGTCAGGCGTTCCGAAAATACCCACTTTACTTTGATGCCCTTTATTGCAACC
TCGTAGGAGCTGGTGAGCAAGCCGGTATTCTGGATTCGCTGCTTGACCGCCTGGCAACCTATAAGGAAAAAATCCTCGCG
ATCAAAAGCAAGATCAAGTCGGCGCTGTTTTACCCCGTCTCCATCATTGTCGTCGCCTTCATCATCACCGCCGTTATCAT
GATCTTCGTGATTCCGGCCTTTAAGGAATTATTCAGCAGCTTTGGCGCGGACCTGCCAGCCCCGACCCTCATCGTCATGG
CTATCTCCGATTTCTTTGTGGAATACTGGTGGGCAATTTTTGGGGCCATTGGTGGCGGCGTATGGTTTTTCTTCTACTCA
TGGAAGCGCTCGATCAAGATGCAAGCGACCATGGACCGGCTTGTTCTTAAAATTCCGGTATTTGGAGAGTTGATACGCAA
GGCCACCATCGCCCGCTTTGCCCGCACCCTCTCCACCATGTTTGCCGCCGGCGTACCACTGGTAGAAGCACTGGACTCCG
TTGCCGGCGCATCGGGCAACCGCGTTTACTATGAGGCAACCAAGCAGATTCAAAGCGAGATCAGCACGGGTACCAGCCTC
ACCGTGGCCATGCAGAATGTAGAAGTTTTCCCCAATATGGTGTTGCAAATGGTCGCCATTGGTGAGGAATCCGGTGCGCT
TGATTCCATGCTATCCAAGGTCGCCGATTTCTACGAAGCAGAAGTGGATGATGCCGTAGAAGCCCTGTCCAGCCTGATGG
AGCCTATCATCATGGTCGTGCTGGGCACCCTGATCGGGGGGCTGGTCATCGCCATGTACCTGCCTATCTTCAAGATGGGG
CAAGTGGTTTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(273-395)

Type II secretion system (T2SS), protein F (T2SSF)

(69-192)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB C6X9L4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Legionella pneumophila strain ERS1305867

55.303

98.263

0.543
  pilC Pseudomonas stutzeri DSM 10701

54.074

100

0.543
  pilC Acinetobacter baylyi ADP1

52.696

100

0.533
  pilC Acinetobacter baumannii D1279779

54.156

98.511

0.533
  pilG Neisseria gonorrhoeae MS11

52.605

100

0.526
  pilG Neisseria meningitidis 44/76-A

52.357

100

0.524
  pilC Vibrio cholerae strain A1552

42.172

98.263

0.414
  pilC Vibrio campbellii strain DS40M4

40.547

99.752

0.404
  pilC Thermus thermophilus HB27

38.75

99.256

0.385

Multiple sequence alignment