Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   KAN02_RS17275 Genome accession   NZ_CP036171
Coordinates   3600371..3602083 (+) Length   570 a.a.
NCBI ID   WP_087605808.1    Uniprot ID   -
Organism   Acinetobacter nosocomialis strain KAN02     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3595371..3607083
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KAN02_RS17240 (KAN02_17240) nusA 3596261..3597745 (-) 1485 WP_002156952.1 transcription termination factor NusA -
  KAN02_RS17245 (KAN02_17245) rimP 3597783..3598307 (-) 525 WP_002157009.1 ribosome maturation factor RimP -
  KAN02_RS17265 (KAN02_17265) secG 3598944..3599273 (-) 330 WP_002156913.1 preprotein translocase subunit SecG -
  KAN02_RS17270 (KAN02_17270) tpiA 3599286..3600080 (-) 795 WP_087605807.1 triose-phosphate isomerase -
  KAN02_RS17275 (KAN02_17275) pilB 3600371..3602083 (+) 1713 WP_087605808.1 type IV-A pilus assembly ATPase PilB Machinery gene
  KAN02_RS17280 (KAN02_17280) pilC 3602111..3603337 (+) 1227 WP_022575843.1 type II secretion system F family protein Machinery gene
  KAN02_RS19355 (KAN02_17285) pilD 3603337..3604197 (+) 861 WP_087605809.1 prepilin peptidase Machinery gene
  KAN02_RS17290 (KAN02_17290) coaE 3604199..3604795 (+) 597 WP_000874125.1 dephospho-CoA kinase -
  KAN02_RS17295 (KAN02_17295) - 3604792..3605706 (-) 915 WP_031952278.1 DMT family transporter -
  KAN02_RS17300 (KAN02_17300) rlmB 3605742..3606491 (-) 750 WP_087605810.1 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB -
  KAN02_RS17305 (KAN02_17305) - 3606593..3606919 (-) 327 WP_000099685.1 pyrimidine/purine nucleoside phosphorylase -

Sequence

Protein


Download         Length: 570 a.a.        Molecular weight: 63427.82 Da        Isoelectric Point: 5.2413

>NTDB_id=347306 KAN02_RS17275 WP_087605808.1 3600371..3602083(+) (pilB) [Acinetobacter nosocomialis strain KAN02]
MSVLHTSPKFTGFFRRLVEEKHVSAAAMQNALDAAKRAKQDTVAYLIEEVHLSPSLLAETISAEFAEPYFDLDVYDTSQI
PKDLVDQKLILKHRILPLIQRGQILYVATSNPSNIEAIDAIRFNSKLLVEPVIVEHHKLEKVLGQHFAEESSFDFNDEEF
DLDVSLDGPTTQEDEEETPQGDEAPIVKYINKLLIDAIRMGASDLHFEPYEKSYRVRYRVDGVLRQIANPPLQLANRLAS
RLKVMSQMDISEKRVPQDGRIKLKLSKTKAIDFRVNSLPTLFGEKLVLRILDPSSAMLGIDALGYEEEQKALFMEALDKP
QGMLLITGPTGSGKTVSLYTGLNILNTESSNISTAEDPVEINLEGINQVNVNPKVGLTFAAALKSFLRQDPDIIMVGEIR
DLETAEIAIKAAQTGHMVMSTLHTNSAPETLTRLRNMGVPSFNIATSVNLVIAQRLARRLCSQCKIPADIPKQSLLEMGF
TEQDLAHPDFRVFQPVGCPECREGYKGRVGIYEVMKVTPEISKIIMEDGNALEIAAASEKLGFNNLRRSGLKKVMQGVTS
LQEVNRVTSE

Nucleotide


Download         Length: 1713 bp        

>NTDB_id=347306 KAN02_RS17275 WP_087605808.1 3600371..3602083(+) (pilB) [Acinetobacter nosocomialis strain KAN02]
ATGTCAGTATTACATACATCTCCAAAATTTACGGGGTTTTTTCGGCGTTTAGTCGAAGAAAAGCATGTGTCGGCAGCGGC
CATGCAAAATGCATTAGATGCAGCAAAGAGAGCCAAGCAGGATACAGTCGCATATTTAATTGAAGAGGTACATCTCTCCC
CTTCTTTATTGGCTGAAACCATTTCTGCGGAGTTTGCTGAACCATATTTTGATCTAGATGTTTATGACACATCTCAGATT
CCTAAAGATCTGGTCGATCAAAAACTGATTTTAAAACATCGAATTTTGCCGCTCATTCAAAGAGGACAAATTCTATATGT
CGCCACCAGCAATCCAAGTAATATTGAAGCAATCGATGCAATCCGTTTTAACAGTAAATTGCTGGTTGAACCTGTTATTG
TCGAGCATCATAAGCTGGAAAAAGTTTTAGGCCAGCATTTTGCAGAAGAAAGCAGCTTTGACTTTAATGATGAAGAGTTT
GATCTTGACGTCAGCCTTGATGGTCCTACCACTCAAGAAGATGAAGAAGAAACACCTCAAGGTGATGAAGCTCCTATTGT
TAAATATATTAATAAGTTGCTTATTGATGCCATTCGTATGGGCGCGTCAGATTTACATTTTGAGCCTTATGAAAAGTCAT
ATCGGGTACGTTATCGTGTCGATGGTGTGTTACGTCAAATTGCCAATCCGCCTTTACAACTTGCAAACCGTTTGGCATCA
CGCTTAAAAGTAATGTCTCAAATGGACATTTCTGAAAAACGCGTCCCACAAGATGGACGTATTAAACTTAAGCTGTCAAA
AACCAAAGCAATTGATTTCCGTGTGAACTCGCTCCCTACTTTGTTCGGCGAAAAGTTAGTTCTACGTATTCTTGACCCAT
CAAGCGCAATGCTAGGCATTGATGCATTAGGCTACGAAGAAGAACAAAAAGCTTTATTTATGGAAGCTTTAGATAAACCA
CAAGGTATGCTTTTGATTACTGGACCAACAGGTTCCGGTAAAACTGTATCTTTATATACAGGCTTAAATATTTTAAATAC
TGAGAGTTCCAATATTTCTACTGCCGAAGATCCAGTGGAAATTAACTTAGAAGGTATTAATCAGGTTAACGTCAATCCTA
AAGTGGGTTTAACGTTTGCAGCTGCACTTAAGTCTTTTTTACGTCAAGACCCTGACATTATTATGGTCGGTGAGATTCGT
GACTTGGAAACAGCAGAAATTGCGATTAAGGCTGCTCAAACCGGCCATATGGTGATGTCAACACTACATACCAATAGTGC
TCCGGAAACATTGACCCGTCTACGCAATATGGGGGTTCCTTCTTTTAATATTGCAACATCAGTAAATCTAGTGATTGCAC
AGCGTTTAGCACGTCGTCTTTGTTCTCAATGTAAAATACCTGCTGATATTCCTAAACAAAGCTTATTAGAAATGGGCTTT
ACCGAACAAGATTTAGCACATCCAGATTTTCGGGTTTTTCAACCCGTGGGCTGTCCTGAATGTCGTGAAGGCTATAAAGG
CCGTGTCGGTATTTATGAGGTAATGAAAGTTACACCTGAAATTTCCAAGATTATTATGGAAGATGGCAACGCTTTAGAAA
TCGCGGCTGCTTCTGAAAAATTGGGGTTTAATAATCTACGTCGTTCAGGTTTAAAAAAGGTGATGCAAGGGGTGACCTCT
TTACAAGAAGTTAACCGTGTAACCAGTGAATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(192-459)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(46-151)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

98.421

100

0.984
  pilB Acinetobacter baylyi ADP1

79.825

100

0.798
  pilB Legionella pneumophila strain ERS1305867

50.794

99.474

0.505
  pilF Neisseria gonorrhoeae MS11

50.178

98.596

0.495
  pilB Vibrio parahaemolyticus RIMD 2210633

51.698

92.982

0.481
  pilB Vibrio campbellii strain DS40M4

50.867

91.053

0.463
  pilB Vibrio cholerae strain A1552

50.29

90.702

0.456
  pilB/pilB1 Synechocystis sp. PCC 6803

35.179

100

0.379

Multiple sequence alignment