Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   EUZ85_RS30430 Genome accession   NZ_CP035490
Coordinates   6840668..6841795 (-) Length   375 a.a.
NCBI ID   WP_127973828.1    Uniprot ID   A0AAE6CKM6
Organism   Hahella sp. KA22     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
ICE 6824903..6859341 6840668..6841795 within 0


Gene organization within MGE regions


Location: 6824903..6859341
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EUZ85_RS30350 (EUZ85_30350) - 6824903..6826159 (-) 1257 WP_127973814.1 site-specific integrase -
  EUZ85_RS30360 (EUZ85_30360) - 6826511..6827377 (-) 867 WP_127973816.1 YicC/YloC family endoribonuclease -
  EUZ85_RS30365 (EUZ85_30365) - 6827637..6828017 (-) 381 WP_011400037.1 hypothetical protein -
  EUZ85_RS30370 (EUZ85_30370) rph 6828299..6829015 (+) 717 WP_127973817.1 ribonuclease PH -
  EUZ85_RS30375 (EUZ85_30375) - 6829104..6829877 (-) 774 WP_127973818.1 exodeoxyribonuclease III -
  EUZ85_RS30380 (EUZ85_30380) pyrE 6829959..6830606 (+) 648 WP_011400040.1 orotate phosphoribosyltransferase -
  EUZ85_RS30385 (EUZ85_30385) - 6830603..6831382 (-) 780 WP_241566900.1 sulfite exporter TauE/SafE family protein -
  EUZ85_RS30390 (EUZ85_30390) - 6831466..6832110 (+) 645 WP_127973820.1 ABC transporter ATPase -
  EUZ85_RS30395 (EUZ85_30395) - 6832178..6832552 (-) 375 WP_127973821.1 hypothetical protein -
  EUZ85_RS30400 (EUZ85_30400) - 6832920..6834206 (+) 1287 WP_127973822.1 MgtC/SapB family protein -
  EUZ85_RS30405 (EUZ85_30405) yegQ 6834238..6835578 (+) 1341 WP_127973823.1 tRNA 5-hydroxyuridine modification protein YegQ -
  EUZ85_RS30410 (EUZ85_30410) - 6835810..6836295 (+) 486 WP_127973824.1 fibronectin type III domain-containing protein -
  EUZ85_RS30415 (EUZ85_30415) - 6836337..6837302 (-) 966 WP_127973825.1 homoserine kinase -
  EUZ85_RS30420 (EUZ85_30420) - 6837387..6837665 (-) 279 WP_127973826.1 DUF2782 domain-containing protein -
  EUZ85_RS30425 (EUZ85_30425) polA 6837852..6840587 (+) 2736 WP_127973827.1 DNA polymerase I -
  EUZ85_RS30430 (EUZ85_30430) pilU 6840668..6841795 (-) 1128 WP_127973828.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  EUZ85_RS30435 (EUZ85_30435) pilT 6841858..6842892 (-) 1035 WP_011400052.1 type IV pilus twitching motility protein PilT Machinery gene
  EUZ85_RS30440 (EUZ85_30440) - 6843050..6843736 (+) 687 WP_127973829.1 YggS family pyridoxal phosphate-dependent enzyme -
  EUZ85_RS30445 (EUZ85_30445) proC 6843839..6844669 (+) 831 WP_127973830.1 pyrroline-5-carboxylate reductase -
  EUZ85_RS30450 (EUZ85_30450) - 6844744..6845283 (+) 540 WP_127973831.1 YggT family protein -
  EUZ85_RS30455 (EUZ85_30455) - 6845276..6845584 (+) 309 WP_127973832.1 DUF167 family protein -
  EUZ85_RS30460 (EUZ85_30460) - 6845770..6847728 (+) 1959 WP_127973833.1 dynamin family protein -
  EUZ85_RS30465 (EUZ85_30465) - 6847902..6849059 (+) 1158 WP_127973834.1 homoserine O-acetyltransferase -
  EUZ85_RS30470 (EUZ85_30470) metW 6849049..6849642 (+) 594 WP_127973835.1 methionine biosynthesis protein MetW -
  EUZ85_RS30475 (EUZ85_30475) - 6849650..6850117 (+) 468 WP_241566901.1 DUF4426 domain-containing protein -
  EUZ85_RS30480 (EUZ85_30480) - 6850162..6850761 (+) 600 WP_127973836.1 XTP/dITP diphosphatase -
  EUZ85_RS30485 (EUZ85_30485) hemW 6850754..6851920 (+) 1167 WP_127973837.1 radical SAM family heme chaperone HemW -
  EUZ85_RS30490 (EUZ85_30490) - 6851980..6852507 (+) 528 WP_127973838.1 hypothetical protein -
  EUZ85_RS30495 (EUZ85_30495) trmB 6852542..6853228 (-) 687 WP_127973839.1 tRNA (guanosine(46)-N7)-methyltransferase TrmB -
  EUZ85_RS30500 (EUZ85_30500) - 6853762..6854613 (+) 852 WP_127973840.1 hypothetical protein -
  EUZ85_RS30505 (EUZ85_30505) - 6854922..6856454 (-) 1533 WP_127973841.1 YifB family Mg chelatase-like AAA ATPase -
  EUZ85_RS30510 (EUZ85_30510) - 6856561..6856839 (-) 279 WP_127973842.1 accessory factor UbiK family protein -
  EUZ85_RS30515 (EUZ85_30515) glnK 6857251..6857589 (+) 339 WP_011400096.1 P-II family nitrogen regulator -
  EUZ85_RS30520 (EUZ85_30520) - 6857641..6858912 (+) 1272 WP_127973843.1 ammonium transporter -
  EUZ85_RS30525 (EUZ85_30525) - 6859090..6859341 (+) 252 WP_127973844.1 SemiSWEET transporter -

Sequence

Protein


Download         Length: 375 a.a.        Molecular weight: 41464.61 Da        Isoelectric Point: 6.8224

>NTDB_id=342790 EUZ85_RS30430 WP_127973828.1 6840668..6841795(-) (pilU) [Hahella sp. KA22]
MDFDKLLKIMVEKGASDLFITAGVPPSIKVHGRMVPITKSPLSPEQTRETVFSIMSEKQRADFEVAHECNFAISARGIGR
FRVSAFYQRNLCGMVLRRIETNIPKLDDLHLPEVIKSLSMTKRGLIIFVGATGTGKSTSLAAMIGHRNRNSKGHIISIED
PIEYIHQHHGCIITQREVGLDTDSFEVALKNTLRQAPDVIMIGEVRSADTMEYAVTFAETGHLCLATLHANNANQALDRI
INFFPPEQHNQVWMDLSLNLKAIVAQQLVPTPDGKGRRAIVEILINSPLAADLIRKGEVHKLKELMTKSSELGMQTFDQA
LYRAYAAGEITYEDALSHADSANDLRLMIKLGADSNISKLQGAASSLSLSDDQDY

Nucleotide


Download         Length: 1128 bp        

>NTDB_id=342790 EUZ85_RS30430 WP_127973828.1 6840668..6841795(-) (pilU) [Hahella sp. KA22]
ATGGATTTCGACAAGCTGTTAAAAATCATGGTGGAAAAGGGGGCTTCGGACCTCTTTATCACAGCTGGGGTGCCGCCCAG
CATCAAAGTTCACGGCCGTATGGTGCCAATCACCAAGTCGCCGCTGTCTCCCGAGCAGACGCGGGAAACCGTGTTTTCCA
TTATGTCGGAAAAGCAGCGTGCGGATTTTGAAGTCGCGCATGAGTGCAACTTTGCAATCAGCGCCCGCGGCATCGGCCGT
TTCCGGGTCAGCGCCTTTTACCAGCGCAATCTGTGCGGGATGGTGTTGCGTCGAATTGAGACCAATATCCCTAAGCTGGA
CGACCTGCATCTGCCGGAGGTTATCAAGTCGCTGTCGATGACCAAGCGGGGCCTGATTATCTTCGTCGGCGCCACCGGCA
CGGGTAAGTCTACGTCGCTGGCTGCGATGATTGGCCACCGGAATCGCAACAGCAAAGGGCACATCATTTCCATCGAGGAC
CCTATCGAATATATCCATCAGCACCACGGTTGCATCATTACGCAACGGGAAGTAGGGCTGGATACGGATTCGTTCGAAGT
GGCCCTGAAGAACACGTTGCGGCAGGCTCCCGACGTCATCATGATCGGGGAGGTGCGCTCGGCCGACACTATGGAATACG
CGGTGACCTTCGCGGAAACCGGTCACTTGTGTTTGGCGACCTTGCACGCCAACAACGCCAACCAGGCGCTGGACCGGATC
ATCAACTTTTTCCCGCCTGAGCAACATAACCAGGTGTGGATGGACTTGTCCTTGAACCTGAAGGCCATTGTGGCGCAGCA
ATTGGTGCCGACTCCTGACGGCAAAGGGCGTCGGGCGATTGTTGAGATCCTCATCAACTCGCCGCTGGCCGCTGACTTGA
TCCGTAAAGGGGAAGTGCACAAGTTGAAAGAGCTGATGACCAAGTCGTCGGAACTGGGCATGCAGACTTTCGATCAGGCG
CTATACCGCGCTTACGCTGCGGGAGAGATCACCTACGAAGACGCGTTGTCTCATGCGGACTCCGCGAACGACCTGCGTCT
GATGATCAAACTGGGCGCAGACAGCAATATTTCCAAGCTGCAAGGCGCAGCCAGCAGTCTGTCATTGAGCGACGACCAGG
ATTATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(113-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

76.471

99.733

0.763
  pilU Acinetobacter baylyi ADP1

64.706

95.2

0.616
  pilU Vibrio cholerae strain A1552

55.647

96.8

0.539
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.773

90.4

0.387
  pilT Pseudomonas aeruginosa PAK

40.708

90.4

0.368
  pilT Acinetobacter baumannii D1279779

40.118

90.4

0.363
  pilT Acinetobacter baumannii strain A118

40.118

90.4

0.363

Multiple sequence alignment