Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   EQU24_RS03345 Genome accession   NZ_CP035467
Coordinates   709700..711418 (+) Length   572 a.a.
NCBI ID   WP_017840855.1    Uniprot ID   A0A4P9UMI0
Organism   Methylotuvimicrobium buryatense strain 5GB1C     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 704700..716418
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EQU24_RS03330 (EQU24_03330) - 704794..706293 (+) 1500 WP_017840858.1 leucyl aminopeptidase -
  EQU24_RS03335 (EQU24_03335) - 706294..706722 (+) 429 WP_017840857.1 DNA polymerase III subunit chi -
  EQU24_RS03340 (EQU24_03340) - 706788..709598 (+) 2811 WP_017840856.1 valine--tRNA ligase -
  EQU24_RS03345 (EQU24_03345) pilB 709700..711418 (+) 1719 WP_017840855.1 type IV-A pilus assembly ATPase PilB Machinery gene
  EQU24_RS03350 (EQU24_03350) pilC 711421..712641 (+) 1221 WP_017840854.1 type II secretion system F family protein Machinery gene
  EQU24_RS03355 (EQU24_03355) pilD 712797..713699 (+) 903 WP_017840853.1 A24 family peptidase Machinery gene
  EQU24_RS03360 (EQU24_03360) coaE 713704..714309 (+) 606 WP_040575649.1 dephospho-CoA kinase -
  EQU24_RS03365 (EQU24_03365) zapD 714313..715083 (+) 771 WP_026130183.1 cell division protein ZapD -
  EQU24_RS03370 (EQU24_03370) yacG 715083..715289 (+) 207 WP_014147184.1 DNA gyrase inhibitor YacG -
  EQU24_RS03375 (EQU24_03375) - 715604..716026 (-) 423 WP_017840850.1 hypothetical protein -

Sequence

Protein


Download         Length: 572 a.a.        Molecular weight: 62770.69 Da        Isoelectric Point: 6.0973

>NTDB_id=342641 EQU24_RS03345 WP_017840855.1 709700..711418(+) (pilB) [Methylotuvimicrobium buryatense strain 5GB1C]
MATDLIDLPIGGLPKCLVQAGLITEADMQLHMQAAKKAKLPLISYLVTNKLVDSKSVTAKASKEFGIPQLDLDALDIRSL
PVNLVSEKLIRKHHALPLLKRGNRLFLAVSDPGNFQAQDEIKFHTRLNIDTILVEEDKLSKAIETSMEAADTTMTDLLDA
DLENLEITGGDQEPAGDEAASDVDDAPIVRFVNKILLDSIKKGVSDIHLEPYEKSFRIRFRADGMLYEVASPPPNIATRI
ISRIKVMAKMDIAERRVPQDGRIKMALSKNRAIDFRVNSCPTLFGEKVVLRILDPTSAQIGIEKLGFEPVQQQRFLEAIN
RPYGMVLVTGPTGSGKTVTLYTGLNLLNSVERNISTAEDPVEITVPGINQVNMNPKAGLTFAGALRAFLRQDPDIIMVGE
IRDLETAEIAVKAAQTGHLVLSTLHTNDAPQTLNRLVQMGVPPFNIVSAVHLIMAQRLARRLCEYCKVKSDLPDSVLISA
GFKEEELAKLKIFTANPAGCEHCTSGYKGRVGIYQVMPISENMRKLILEGGNTMQLAEQAKAEGINDLRASGLEKVCQGI
TSLEEIDRVTKE

Nucleotide


Download         Length: 1719 bp        

>NTDB_id=342641 EQU24_RS03345 WP_017840855.1 709700..711418(+) (pilB) [Methylotuvimicrobium buryatense strain 5GB1C]
ATGGCAACAGATTTAATCGACCTGCCGATCGGCGGTCTACCGAAATGTTTGGTCCAAGCCGGCTTGATTACCGAGGCGGA
TATGCAATTGCATATGCAGGCTGCGAAGAAAGCCAAATTACCGCTGATCAGTTATTTGGTCACCAATAAATTGGTTGACA
GCAAATCGGTCACCGCCAAAGCATCCAAAGAGTTCGGTATTCCGCAACTCGATCTCGACGCGCTCGATATTCGTAGCTTG
CCGGTCAATTTGGTCAGTGAAAAACTGATTAGAAAGCATCATGCTTTACCGTTGCTTAAGCGCGGAAACCGCTTGTTTTT
AGCGGTTTCCGATCCCGGCAATTTTCAAGCTCAAGACGAAATAAAGTTCCATACGCGCCTGAATATCGACACGATTCTGG
TCGAAGAAGATAAGTTGTCTAAGGCTATCGAGACATCAATGGAAGCTGCCGATACCACGATGACCGATTTGCTGGATGCC
GACCTGGAGAATTTAGAAATTACCGGCGGCGATCAGGAGCCGGCCGGAGACGAGGCGGCAAGTGATGTGGACGATGCGCC
TATCGTACGTTTCGTCAATAAAATTTTGCTCGATTCGATTAAAAAAGGTGTTTCGGATATTCATCTTGAGCCTTATGAAA
AAAGTTTCCGAATTCGCTTTCGGGCAGACGGCATGCTTTATGAAGTGGCAAGTCCGCCTCCCAATATCGCAACTCGGATT
ATTTCCCGTATCAAGGTCATGGCTAAAATGGATATCGCCGAACGGCGCGTTCCTCAGGACGGGCGGATTAAGATGGCATT
GTCGAAAAATCGGGCAATCGACTTTCGGGTCAATAGCTGTCCGACGTTGTTCGGCGAAAAAGTCGTACTGCGTATACTCG
ATCCGACCAGCGCGCAAATCGGTATCGAAAAACTCGGCTTTGAACCCGTCCAACAACAACGATTTCTTGAGGCGATCAAT
CGGCCTTACGGCATGGTTTTGGTAACGGGTCCTACCGGTAGCGGTAAAACCGTTACCCTTTACACGGGCTTGAATCTGCT
AAATAGCGTCGAACGGAATATATCGACCGCCGAGGACCCTGTCGAAATTACGGTGCCGGGTATCAATCAGGTTAATATGA
ATCCGAAAGCCGGGTTGACCTTTGCCGGTGCCTTGCGGGCTTTTCTACGACAAGATCCGGATATCATTATGGTCGGTGAG
ATACGGGATTTGGAAACCGCCGAAATTGCCGTCAAGGCCGCACAAACGGGCCATTTGGTATTGTCCACGCTGCACACAAA
CGATGCGCCGCAAACGTTAAACCGTCTCGTGCAAATGGGCGTGCCGCCGTTTAACATCGTTTCAGCGGTGCATTTGATCA
TGGCTCAACGCCTCGCCAGACGCTTATGCGAATATTGTAAGGTTAAGTCCGATTTGCCGGATTCGGTGTTGATTTCGGCC
GGTTTCAAAGAAGAAGAGCTAGCTAAGCTTAAAATTTTTACGGCTAATCCTGCTGGCTGCGAACATTGTACTAGCGGCTA
CAAAGGCCGAGTCGGAATCTATCAGGTTATGCCGATTAGCGAAAATATGCGTAAACTGATTCTGGAAGGCGGCAATACTA
TGCAATTAGCCGAACAAGCCAAAGCCGAAGGCATCAACGACTTGCGCGCGTCGGGTCTTGAGAAAGTCTGTCAAGGCATT
ACTAGCCTGGAAGAAATCGACCGGGTCACTAAGGAGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(194-461)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(46-149)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A4P9UMI0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Legionella pneumophila strain ERS1305867

57.27

98.601

0.565
  pilB Acinetobacter baumannii D1279779

53.546

98.601

0.528
  pilB Acinetobacter baylyi ADP1

53.274

98.776

0.526
  pilB Vibrio cholerae strain A1552

48.932

98.252

0.481
  pilF Neisseria gonorrhoeae MS11

47.788

98.776

0.472
  pilB Vibrio campbellii strain DS40M4

47.772

98.077

0.469
  pilB Vibrio parahaemolyticus RIMD 2210633

47.594

98.077

0.467
  pilF Thermus thermophilus HB27

38.569

100

0.386
  pilB/pilB1 Synechocystis sp. PCC 6803

42.085

90.559

0.381

Multiple sequence alignment