Detailed information    

insolico Bioinformatically predicted

Overview

Name   clpP   Type   Regulator
Locus tag   ES963_RS18565 Genome accession   NZ_CP035390
Coordinates   3438821..3439414 (+) Length   197 a.a.
NCBI ID   WP_003228214.1    Uniprot ID   A0A199WEF2
Organism   Bacillus subtilis strain SRCM103641     
Function   degradation of ComK; degradation of DegU (predicted from homology)   
Competence regulation

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
ICE 3399086..3438522 3438821..3439414 flank 299


Gene organization within MGE regions


Location: 3399086..3439414
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ES963_RS18305 (ES963_18305) - 3399278..3399511 (-) 234 WP_033881229.1 helix-turn-helix transcriptional regulator -
  ES963_RS18310 (ES963_18310) - 3399781..3400068 (+) 288 WP_225910697.1 hypothetical protein -
  ES963_RS18315 (ES963_18315) - 3400081..3401265 (+) 1185 WP_014480972.1 FtsK/SpoIIIE domain-containing protein -
  ES963_RS18320 (ES963_18320) - 3401252..3401884 (+) 633 WP_129093131.1 replication-relaxation family protein -
  ES963_RS18325 (ES963_18325) - 3401926..3402228 (-) 303 WP_014480974.1 hypothetical protein -
  ES963_RS18330 (ES963_18330) - 3402350..3402607 (-) 258 WP_014480975.1 holin -
  ES963_RS18335 (ES963_18335) - 3402627..3403574 (-) 948 WP_033881228.1 N-acetylmuramoyl-L-alanine amidase -
  ES963_RS18340 (ES963_18340) - 3403647..3403850 (-) 204 WP_033881227.1 BhlA/UviB family holin-like peptide -
  ES963_RS18345 (ES963_18345) - 3403875..3404045 (-) 171 WP_129093132.1 XkdX family protein -
  ES963_RS18350 (ES963_18350) - 3404046..3404339 (-) 294 WP_014480978.1 hypothetical protein -
  ES963_RS18355 (ES963_18355) - 3404355..3405578 (-) 1224 WP_250635435.1 BppU family phage baseplate upper protein -
  ES963_RS18360 (ES963_18360) - 3405615..3407192 (-) 1578 WP_129093133.1 hypothetical protein -
  ES963_RS18365 (ES963_18365) - 3407205..3408599 (-) 1395 WP_031600997.1 phage tail protein -
  ES963_RS18370 (ES963_18370) - 3408614..3410032 (-) 1419 WP_033880944.1 glucosaminidase domain-containing protein -
  ES963_RS18375 (ES963_18375) - 3410036..3412858 (-) 2823 WP_129093134.1 phage tail tape measure protein -
  ES963_RS18380 (ES963_18380) - 3412863..3413084 (-) 222 WP_033880947.1 hypothetical protein -
  ES963_RS18385 (ES963_18385) - 3413180..3413563 (-) 384 WP_017695225.1 hypothetical protein -
  ES963_RS18390 (ES963_18390) - 3413623..3414192 (-) 570 WP_033880949.1 hypothetical protein -
  ES963_RS18395 (ES963_18395) - 3414233..3414607 (-) 375 WP_033880952.1 minor capsid protein -
  ES963_RS18400 (ES963_18400) - 3414612..3415019 (-) 408 WP_033880954.1 hypothetical protein -
  ES963_RS18405 (ES963_18405) - 3415016..3415339 (-) 324 WP_228224819.1 hypothetical protein -
  ES963_RS18410 (ES963_18410) - 3415355..3415747 (-) 393 WP_031601003.1 hypothetical protein -
  ES963_RS18415 (ES963_18415) - 3415765..3415956 (-) 192 WP_033880957.1 hypothetical protein -
  ES963_RS18420 (ES963_18420) - 3416013..3416921 (-) 909 WP_129093135.1 major capsid protein -
  ES963_RS18425 (ES963_18425) - 3416953..3417513 (-) 561 WP_129093136.1 ribonucleoside-triphosphate reductase -
  ES963_RS18430 (ES963_18430) - 3417619..3418431 (-) 813 WP_129093137.1 phage minor capsid protein -
  ES963_RS18435 (ES963_18435) - 3418431..3420101 (-) 1671 WP_031601006.1 phage portal protein -
  ES963_RS18440 (ES963_18440) - 3420106..3420540 (-) 435 WP_031601007.1 phBC6A51 family helix-turn-helix protein -
  ES963_RS18445 (ES963_18445) terL 3420557..3422317 (-) 1761 WP_129093138.1 phage terminase large subunit -
  ES963_RS18450 (ES963_18450) - 3422401..3422949 (+) 549 WP_033880961.1 site-specific integrase -
  ES963_RS18455 (ES963_18455) - 3422979..3423188 (-) 210 WP_031601009.1 hypothetical protein -
  ES963_RS22185 - 3423720..3423881 (-) 162 WP_164971719.1 hypothetical protein -
  ES963_RS18460 (ES963_18460) - 3423883..3424149 (-) 267 WP_077736277.1 hypothetical protein -
  ES963_RS18465 (ES963_18465) - 3424411..3424590 (-) 180 WP_033880962.1 hypothetical protein -
  ES963_RS18470 (ES963_18470) - 3425633..3426175 (-) 543 WP_031601012.1 hypothetical protein -
  ES963_RS18475 (ES963_18475) - 3426172..3426450 (-) 279 WP_017695241.1 hypothetical protein -
  ES963_RS22525 - 3426447..3427238 (-) 792 WP_017695242.1 hypothetical protein -
  ES963_RS18485 (ES963_18485) - 3427311..3427682 (-) 372 WP_129093139.1 sigma factor-like helix-turn-helix DNA-binding protein -
  ES963_RS22635 (ES963_18490) - 3428130..3428378 (-) 249 WP_129093140.1 helix-turn-helix transcriptional regulator -
  ES963_RS18495 (ES963_18495) - 3428851..3429051 (-) 201 WP_129093141.1 hypothetical protein -
  ES963_RS18500 (ES963_18500) - 3429044..3429370 (-) 327 WP_129093142.1 hypothetical protein -
  ES963_RS18505 (ES963_18505) - 3429408..3429950 (-) 543 WP_129093143.1 hypothetical protein -
  ES963_RS22190 - 3429943..3430080 (-) 138 WP_164971720.1 hypothetical protein -
  ES963_RS18510 (ES963_18510) - 3430484..3431659 (-) 1176 WP_129093144.1 AimR family lysis-lysogeny pheromone receptor -
  ES963_RS18515 (ES963_18515) - 3431779..3431994 (+) 216 WP_129093145.1 helix-turn-helix transcriptional regulator -
  ES963_RS18520 (ES963_18520) - 3432186..3432521 (-) 336 WP_129093146.1 hypothetical protein -
  ES963_RS18525 (ES963_18525) - 3432707..3432946 (+) 240 WP_129093147.1 helix-turn-helix domain-containing protein -
  ES963_RS18530 (ES963_18530) - 3433091..3434071 (-) 981 WP_129093148.1 tyrosine-type recombinase/integrase -
  ES963_RS18535 (ES963_18535) - 3434702..3435511 (-) 810 WP_129093149.1 RNA ligase family protein -
  ES963_RS18540 (ES963_18540) - 3435598..3436020 (-) 423 WP_129093150.1 hypothetical protein -
  ES963_RS18545 (ES963_18545) - 3436036..3436218 (-) 183 WP_129093151.1 hypothetical protein -
  ES963_RS18550 (ES963_18550) - 3436392..3437126 (-) 735 WP_129093199.1 helix-turn-helix domain-containing protein -
  ES963_RS18555 (ES963_18555) - 3437286..3438275 (-) 990 WP_014480981.1 tyrosine-type recombinase/integrase -
  ES963_RS18565 (ES963_18565) clpP 3438821..3439414 (+) 594 WP_003228214.1 ATP-dependent Clp endopeptidase proteolytic subunit ClpP Regulator

Sequence

Protein


Download         Length: 197 a.a.        Molecular weight: 21682.00 Da        Isoelectric Point: 4.9416

>NTDB_id=339568 ES963_RS18565 WP_003228214.1 3438821..3439414(+) (clpP) [Bacillus subtilis strain SRCM103641]
MNLIPTVIEQTNRGERAYDIYSRLLKDRIIMLGSAIDDNVANSIVSQLLFLAAEDPEKEISLYINSPGGSITAGMAIYDT
MQFIKPKVSTICIGMAASMGAFLLAAGEKGKRYALPNSEVMIHQPLGGAQGQATEIEIAAKRILLLRDKLNKVLAERTGQ
PLEVIERDTDRDNFKSAEEALEYGLIDKILTHTEDKK

Nucleotide


Download         Length: 594 bp        

>NTDB_id=339568 ES963_RS18565 WP_003228214.1 3438821..3439414(+) (clpP) [Bacillus subtilis strain SRCM103641]
ATGAATTTAATACCTACAGTCATTGAACAAACGAACCGCGGGGAAAGAGCGTATGACATTTATTCTCGTCTATTAAAGGA
TCGTATCATCATGCTTGGATCTGCGATTGATGACAACGTTGCGAACTCCATCGTGTCACAGCTTTTATTCTTAGCAGCAG
AAGACCCTGAAAAAGAAATTTCTCTTTACATCAACAGCCCGGGCGGCTCTATTACAGCCGGTATGGCGATCTATGATACC
ATGCAGTTTATTAAGCCGAAGGTATCTACAATTTGTATCGGTATGGCTGCGTCAATGGGCGCGTTCCTGCTTGCAGCCGG
CGAAAAAGGCAAACGCTATGCGCTTCCAAACAGTGAAGTCATGATTCACCAGCCTCTTGGCGGTGCGCAAGGTCAAGCGA
CAGAAATTGAAATTGCCGCGAAACGCATTCTCTTGCTTCGCGACAAATTAAACAAAGTCCTTGCTGAACGTACTGGCCAG
CCGCTTGAAGTGATCGAACGCGACACAGACCGTGATAACTTCAAGTCTGCTGAAGAAGCGCTTGAATACGGCCTGATTGA
CAAAATTTTGACTCACACAGAAGACAAAAAGTAA

Domains

Predicted by InterproScan.

Clp protease (CLP_protease)

(13-192)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A199WEF2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  clpP Bacillus subtilis subsp. subtilis str. 168

100

100

1
  clpP Campylobacter jejuni subsp. jejuni NCTC 11168 = ATCC 700819

68.617

95.431

0.655
  clpP Streptococcus thermophilus LMG 18311

58.163

99.492

0.579
  clpP Streptococcus thermophilus LMD-9

58.163

99.492

0.579
  clpP Streptococcus pneumoniae D39

57.812

97.462

0.563
  clpP Streptococcus pneumoniae Rx1

57.812

97.462

0.563
  clpP Streptococcus pneumoniae R6

57.812

97.462

0.563
  clpP Streptococcus pneumoniae TIGR4

57.812

97.462

0.563
  clpP Lactococcus lactis subsp. cremoris KW2

57.895

96.447

0.558
  clpP Streptococcus pyogenes JRS4

56.122

99.492

0.558
  clpP Streptococcus pyogenes MGAS315

56.122

99.492

0.558
  clpP Streptococcus mutans UA159

54.822

100

0.548
  clpP Lactococcus lactis subsp. lactis strain DGCC12653

55.789

96.447

0.538

Multiple sequence alignment