Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   EKL02_RS04515 Genome accession   NZ_CP035311
Coordinates   1004176..1005903 (-) Length   575 a.a.
NCBI ID   WP_128900933.1    Uniprot ID   A0A410UQ79
Organism   Janthinobacterium sp. 17J80-10     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 999176..1010903
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EKL02_RS04485 (EKL02_04485) - 999983..1000393 (+) 411 WP_128900927.1 NUDIX domain-containing protein -
  EKL02_RS04490 (EKL02_04490) yacG 1000404..1000580 (-) 177 WP_128900928.1 DNA gyrase inhibitor YacG -
  EKL02_RS04495 (EKL02_04495) zapD 1000595..1001350 (-) 756 WP_128900929.1 cell division protein ZapD -
  EKL02_RS04500 (EKL02_04500) coaE 1001437..1002045 (-) 609 WP_128900930.1 dephospho-CoA kinase -
  EKL02_RS04505 (EKL02_04505) pilD 1002060..1002926 (-) 867 WP_128900931.1 A24 family peptidase Machinery gene
  EKL02_RS04510 (EKL02_04510) pilC 1002926..1004158 (-) 1233 WP_128900932.1 type II secretion system F family protein Machinery gene
  EKL02_RS04515 (EKL02_04515) pilB 1004176..1005903 (-) 1728 WP_128900933.1 type IV-A pilus assembly ATPase PilB Machinery gene
  EKL02_RS18120 - 1006153..1006329 (-) 177 WP_164931949.1 NF038104 family lipoprotein -
  EKL02_RS04525 (EKL02_04525) ispB 1006602..1007531 (-) 930 WP_241687848.1 octaprenyl diphosphate synthase -
  EKL02_RS04530 (EKL02_04530) rplU 1007839..1008150 (+) 312 WP_119787204.1 50S ribosomal protein L21 -
  EKL02_RS04535 (EKL02_04535) rpmA 1008188..1008445 (+) 258 WP_128900935.1 50S ribosomal protein L27 -
  EKL02_RS04540 (EKL02_04540) cgtA 1008547..1009656 (+) 1110 WP_128900936.1 Obg family GTPase CgtA -
  EKL02_RS04545 (EKL02_04545) proB 1009720..1010838 (+) 1119 WP_128900937.1 glutamate 5-kinase -

Sequence

Protein


Download         Length: 575 a.a.        Molecular weight: 62803.36 Da        Isoelectric Point: 5.0529

>NTDB_id=339262 EKL02_RS04515 WP_128900933.1 1004176..1005903(-) (pilB) [Janthinobacterium sp. 17J80-10]
MAAVFPNTVSSTAIPALARVLLHAGRISAQQVDALTKRTQSEKISFIDALVQSGYIEPRALAAFCSETFAYPVLDIATFS
LNTRPEGLIDPKLMQSQRVIALAKRGNKISVAISDPTNSQALDQIKFQTQMSVEPVIVEHPALLQLVEKLGQSAEQNLNE
LIGDDLDIDFAEVDTTTTTESGPTADIDDAPVVRFLQKILIDAINAGASDLHFEPFEKFYRIRLRVDGVLKEVAQPPLAI
KEKLASRIKVISKLDISEKRVPQDGRMKLVVSKTKSIDFRVSTLPTLFGEKIVMRILDATQAQMGIDALGYDPDQKEILL
NAIERPYGMVLVTGPTGSGKTVSLYTCLNILNKPGINISTAEDPAEINLPGVNQVNVNDRAGLTFPVALKAFLRQDPDII
MVGEIRDLETADIAIKAAQTGHMVFSTLHTNDAPATLTRLMNMGVAPFNIASSVILITAQRLARRLCTCKQPTTIPDEAL
LEAGFKEEEIDGAWQPYKAVGCERCSGSGYKGRVGIYQVMPISEEIERIILAHGTALEIEEQSRREGIRTLRESGLVKVK
LGFTSLEEVLGCTNE

Nucleotide


Download         Length: 1728 bp        

>NTDB_id=339262 EKL02_RS04515 WP_128900933.1 1004176..1005903(-) (pilB) [Janthinobacterium sp. 17J80-10]
ATGGCGGCAGTTTTTCCCAACACGGTTTCCAGCACGGCGATTCCTGCTTTGGCCAGAGTATTGCTCCATGCCGGCCGCAT
TTCCGCTCAGCAAGTGGATGCTTTAACCAAGCGGACGCAGTCGGAAAAGATCTCCTTTATTGATGCATTGGTGCAAAGCG
GCTATATCGAGCCACGCGCTCTGGCGGCATTTTGCTCAGAAACATTTGCCTATCCTGTTCTGGATATTGCCACCTTCAGC
CTGAATACGCGTCCGGAAGGCTTGATCGACCCCAAACTGATGCAAAGCCAGCGGGTCATCGCGCTGGCCAAGCGCGGCAA
CAAGATTTCGGTAGCAATTTCCGACCCGACCAACAGTCAGGCCCTCGACCAGATCAAGTTCCAGACTCAAATGTCGGTTG
AGCCCGTCATTGTCGAGCATCCGGCGCTCTTGCAACTGGTCGAAAAACTCGGCCAAAGCGCTGAGCAAAATCTGAATGAG
TTGATCGGCGATGACCTGGATATCGATTTTGCCGAAGTCGACACGACGACTACCACGGAAAGCGGGCCAACCGCCGATAT
TGACGATGCGCCGGTTGTCCGGTTCCTGCAAAAAATCCTGATCGATGCCATTAACGCTGGCGCCTCGGACTTGCATTTCG
AACCCTTTGAAAAGTTTTACCGCATTCGCTTGCGCGTCGACGGCGTATTGAAGGAAGTCGCGCAACCGCCATTGGCGATC
AAGGAAAAGCTGGCCTCGCGCATCAAGGTCATTTCCAAGCTCGACATTTCGGAAAAACGCGTGCCGCAGGATGGCCGCAT
GAAGCTGGTGGTCTCGAAGACCAAGTCAATCGACTTCCGCGTCAGCACGCTGCCCACGCTGTTTGGCGAAAAAATCGTCA
TGCGTATTCTGGATGCCACCCAGGCGCAAATGGGCATCGATGCGCTCGGCTATGACCCGGACCAGAAGGAAATCCTGCTC
AACGCCATTGAACGGCCTTATGGCATGGTACTGGTGACCGGGCCGACCGGCTCGGGCAAAACAGTGTCGCTGTATACCTG
CCTGAACATCCTGAACAAGCCCGGAATCAATATTTCGACTGCGGAAGACCCGGCCGAAATCAACTTACCAGGCGTAAACC
AGGTCAACGTCAACGACCGTGCCGGCCTGACTTTCCCAGTAGCGCTGAAAGCATTCCTCCGGCAGGATCCGGACATTATC
ATGGTGGGTGAAATCCGCGACCTGGAAACCGCGGATATTGCCATCAAGGCGGCGCAAACCGGCCATATGGTGTTTTCCAC
GCTGCATACCAACGATGCGCCGGCAACGCTGACGCGACTGATGAACATGGGCGTGGCGCCATTCAACATTGCATCGTCGG
TGATCCTGATCACCGCACAACGCCTGGCCCGGCGCCTGTGTACCTGCAAGCAGCCTACCACCATCCCGGACGAAGCGCTG
CTGGAAGCGGGTTTCAAGGAAGAAGAAATCGATGGTGCCTGGCAGCCCTACAAGGCTGTCGGCTGCGAACGCTGCAGCGG
CAGCGGCTACAAGGGCCGTGTGGGCATTTATCAGGTCATGCCGATTTCTGAAGAGATCGAGCGCATCATCCTGGCCCATG
GCACGGCGCTGGAAATCGAAGAACAATCGCGGCGCGAAGGTATCAGGACCTTGCGGGAATCTGGCCTGGTCAAGGTCAAA
CTGGGATTTACCAGCCTGGAAGAAGTCCTGGGTTGCACCAACGAATGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(198-465)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(50-155)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A410UQ79

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

54.758

96.87

0.53
  pilB Acinetobacter baylyi ADP1

54.464

97.391

0.53
  pilF Neisseria gonorrhoeae MS11

53.996

97.913

0.529
  pilB Legionella pneumophila strain ERS1305867

50.926

93.913

0.478
  pilB Vibrio cholerae strain A1552

47.695

98.087

0.468
  pilB Vibrio campbellii strain DS40M4

45.567

98.087

0.447
  pilB Vibrio parahaemolyticus RIMD 2210633

45.018

97.739

0.44
  pilF Thermus thermophilus HB27

39.823

98.261

0.391
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.292

98.261

0.386

Multiple sequence alignment