Detailed information NTG0337585

Bioinformatically predicted

Overview

• Name: comEA/celA/cilE
• Type: Machinery gene
• Locus tag: EQH32_RS04500
• Genome accession: NZ_CP035248
• Coordinates: 886538..887188 (+)
• Length: 216 a.a.
• NCBI ID: WP_000387352.1
• Uniprot ID: A0A4J1SCX2
• Organism: Streptococcus pneumoniae strain TVO_1901939
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 881538..892188

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
EQH32_RS04470 (EQH32_04715)	-	882034..882249 (+)	216	WP_001232078.1	YozE family protein	-
EQH32_RS04475 (EQH32_04720)	-	882335..883303 (+)	969	WP_000658181.1	PhoH family protein	-
EQH32_RS04480 (EQH32_04725)	-	883483..883983 (+)	501	WP_000566988.1	GNAT family N-acetyltransferase	-
EQH32_RS04485 (EQH32_04730)	-	883986..884312 (+)	327	Protein_897	TfoX/Sxy family protein	-
EQH32_RS04490 (EQH32_04735)	ald	884613..885724 (-)	1112	Protein_898	alanine dehydrogenase	-
EQH32_RS04495 (EQH32_04740)	-	885901..886470 (+)	570	WP_000443745.1	GNAT family N-acetyltransferase	-
EQH32_RS04500 (EQH32_04745)	comEA/celA/cilE	886538..887188 (+)	651	WP_000387352.1	helix-hairpin-helix domain-containing protein	Machinery gene
EQH32_RS04505 (EQH32_04750)	comEC/celB	887172..889412 (+)	2241	WP_000942422.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
EQH32_RS04510 (EQH32_04755)	-	889592..889780 (+)	189	WP_010976492.1	hypothetical protein	-
EQH32_RS04515 (EQH32_04760)	-	889812..890399 (+)	588	WP_000933542.1	ATP-binding cassette domain-containing protein	-
EQH32_RS04520 (EQH32_04765)	-	890403..891578 (+)	1176	WP_000655940.1	hypothetical protein	-

Sequence

Protein

• Length: 216 a.a.
• Molecular weight: 23085.30 Da
• Isoelectric Point: 6.0661

>NTDB_id=337585 EQH32_RS04500 WP_000387352.1 886538..887188(+) (comEA/celA/cilE) [Streptococcus pneumoniae strain TVO_1901939]
MEAIIEKIKEYKIIVICTGLGLLVGGFFLLKPAPQTSVKETNLQAEVAAVSKDSSTGKEVNKEEKEEPVEQDLITVDVKG
AVKSPGIYDLPVGSRVNDAVQKAGGLTEQADSKSLNLAQKVSDEALVYVPTKGEEAVSQQTGSGTASSTSKEKKVNLNKA
SLEELKQVKGLGGKRAQDIIDHREANGKFKSVDELKKVSGIGAKTIEKLKDYVTVD

Nucleotide

• Length: 651 bp

>NTDB_id=337585 EQH32_RS04500 WP_000387352.1 886538..887188(+) (comEA/celA/cilE) [Streptococcus pneumoniae strain TVO_1901939]
ATGGAAGCAATTATCGAGAAAATCAAAGAGTATAAAATCATCGTCATCTGTACTGGTCTGGGCTTGCTTGTAGGAGGATT
TTTCCTGCTAAAGCCAGCTCCACAAACATCTGTCAAAGAGACGAATTTGCAGGCTGAAGTTGCAGCTGTTTCCAAGGACT
CATCGACCGGAAAGGAAGTGAACAAGGAAGAGAAGGAAGAACCAGTTGAACAAGATCTAATCACAGTAGATGTCAAAGGT
GCTGTCAAATCGCCAGGGATTTATGACTTGCCTGTAGGTAGTCGAGTCAATGATGCTGTTCAGAAGGCTGGTGGCTTGAC
AGAGCAAGCAGACAGCAAGTCGCTCAATCTAGCTCAGAAAGTTAGTGATGAGGCTCTGGTTTACGTTCCTACTAAGGGAG
AAGAAGCAGTTAGTCAACAGACTGGTTCGGGGACAGCTTCTTCAACAAGCAAGGAAAAGAAGGTCAATCTCAACAAGGCC
AGTCTGGAAGAACTCAAGCAGGTCAAGGGACTGGGAGGAAAACGAGCTCAGGACATTATTGACCATCGTGAGGCAAATGG
CAAGTTCAAGTCAGTAGACGAGCTCAAGAAGGTCTCTGGCATTGGTGCCAAGACCATAGAAAAACTTAAAGACTATGTTA
CAGTGGATTAA

Domains

Predicted by InterproScan.

• Helix-hairpin-helix motif (HHH_3) (151-214)

• SLBB domain (SLBB) (76-126)

3D structure

• AlphaFold DB: A0A4J1SCX2

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	97.685	100	0.977
comEA/celA/cilE	Streptococcus pneumoniae Rx1	95.833	100	0.958
comEA/celA/cilE	Streptococcus pneumoniae D39	95.833	100	0.958
comEA/celA/cilE	Streptococcus pneumoniae R6	95.833	100	0.958
comEA/celA/cilE	Streptococcus mitis NCTC 12261	95.37	100	0.954
comEA/celA/cilE	Streptococcus mitis SK321	92.593	100	0.926
comEA	Lactococcus lactis subsp. cremoris KW2	44.053	100	0.463
comEA	Latilactobacillus sakei subsp. sakei 23K	34.335	100	0.37