Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   FYA20_RS16980 Genome accession   NZ_CP043181
Coordinates   3510761..3512146 (-) Length   461 a.a.
NCBI ID   WP_001025178.1    Uniprot ID   A0A0H2YVM6
Organism   Escherichia coli O2:H6 strain PG20180057     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3505761..3517146
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FYA20_RS16950 (FYA20_16950) mutT 3506210..3506608 (+) 399 WP_000736022.1 8-oxo-dGTP diphosphatase MutT -
  FYA20_RS16955 (FYA20_16955) yacG 3506696..3506893 (-) 198 WP_000005042.1 DNA gyrase inhibitor YacG -
  FYA20_RS16960 (FYA20_16960) zapD 3506903..3507646 (-) 744 WP_001194731.1 cell division protein ZapD -
  FYA20_RS16965 (FYA20_16965) coaE 3507646..3508266 (-) 621 WP_001269520.1 dephospho-CoA kinase -
  FYA20_RS25180 - 3508291..3508335 (+) 45 WP_120795372.1 protein YacM -
  FYA20_RS16970 (FYA20_16970) guaC 3508491..3509534 (+) 1044 WP_001217343.1 GMP reductase -
  FYA20_RS16975 (FYA20_16975) hofC 3509569..3510771 (-) 1203 WP_000157292.1 protein transport protein HofC -
  FYA20_RS16980 (FYA20_16980) pilB 3510761..3512146 (-) 1386 WP_001025178.1 type II secretion system protein GspE Machinery gene
  FYA20_RS16985 (FYA20_16985) pilA 3512156..3512596 (-) 441 WP_000360913.1 prepilin peptidase-dependent pilin Machinery gene
  FYA20_RS16990 (FYA20_16990) nadC 3512800..3513693 (-) 894 WP_001135168.1 carboxylating nicotinate-nucleotide diphosphorylase -
  FYA20_RS16995 (FYA20_16995) ampD 3513781..3514332 (+) 552 WP_000923726.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  FYA20_RS17000 (FYA20_17000) ampE 3514329..3515183 (+) 855 WP_000171984.1 beta-lactamase regulator AmpE -
  FYA20_RS17005 (FYA20_17005) aroP 3515226..3516596 (-) 1371 WP_000399017.1 aromatic amino acid transporter AroP -

Sequence

Protein


Download         Length: 461 a.a.        Molecular weight: 50632.29 Da        Isoelectric Point: 6.5396

>NTDB_id=334450 FYA20_RS16980 WP_001025178.1 3510761..3512146(-) (pilB) [Escherichia coli O2:H6 strain PG20180057]
MNIPQLTALCLRYQGVLLDASEEVVHVAVVDAPSHELLDALHFATTKRIEITCWTRQQMEGHASRTQQTLPVAVQEKHQP
KAELLTRTLQSALEQRASDIHIEPADNAYRIRLRIDGVLHPLPDVSPDAGVALTARLKVLGNLDIAEHRLPQDGQFTVEL
AGNAVSFRIATLACRGGEKVVLRLLQQVNQALDVNTLGMQPSQLVDFAHALQQPQGLVLVTGPTGSGKTVTLYSALQMLN
TADINICSVEDPVEIPIAGLNQTQIHSRAGLTFQGVLRALLRQDPDVIMIGEIRDGETAEIAIKAAQTGHLVLSTLHTNS
TCETLVRLQQMGVARWMLSSALTLVIAQRLVRKLCPHCRRQQGEPIHIPDNVWPSPLPHWQAPGCVHCYHGFYGRTALFE
VLPITPVIRQLISANTDVESLETHARQAGMRTLFENGCLAVEQGLTTFEELIRVLGMPHGE

Nucleotide


Download         Length: 1386 bp        

>NTDB_id=334450 FYA20_RS16980 WP_001025178.1 3510761..3512146(-) (pilB) [Escherichia coli O2:H6 strain PG20180057]
ATGAATATTCCACAGCTCACTGCCCTGTGTCTGCGTTATCAGGGAGTCTTGCTGGATGCCAGCGAAGAGGTGGTTCATGT
TGCGGTAGTCGATGCACCTTCGCATGAGCTACTGGACGCATTGCATTTCGCTACCACCAAACGTATTGAGATCACCTGCT
GGACACGCCAACAAATGGAAGGTCACGCCAGTCGCACACAACAGACATTGCCCGTAGCTGTTCAGGAGAAGCATCAGCCC
AAAGCAGAGTTGCTGACTCGAACGTTACAATCTGCGCTGGAACAACGCGCGTCTGATATTCATATCGAACCAGCGGACAA
TGCCTACCGCATCCGCTTGCGTATCGACGGCGTATTGCATCCTTTACCGGACGTTTCACCGGATGCCGGAGTCGCATTAA
CCGCCAGATTAAAAGTGCTGGGAAACCTGGATATTGCGGAACATCGCCTGCCGCAGGACGGGCAATTCACTGTCGAACTG
GCAGGAAACGCCGTCTCATTTCGTATTGCGACCTTAGCATGTCGGGGTGGTGAAAAGGTGGTATTAAGGTTGTTACAGCA
GGTGAACCAGGCACTGGATGTTAACACGCTTGGAATGCAGCCGTCACAACTGGTGGACTTTGCTCATGCCTTGCAACAAC
CACAGGGACTGGTGCTGGTAACTGGCCCTACAGGCAGCGGCAAAACGGTCACGCTTTATAGTGCCCTGCAAATGCTGAAT
ACCGCTGACATTAATATTTGTAGCGTCGAAGATCCGGTTGAGATCCCCATAGCCGGACTAAACCAGACGCAAATCCATTC
GCGTGCCGGACTCACCTTTCAGGGCGTTTTGCGTGCGTTATTGCGCCAGGATCCTGACGTCATCATGATCGGAGAGATCC
GCGATGGCGAAACGGCAGAAATTGCCATTAAAGCCGCGCAAACCGGTCACCTGGTGTTGTCTACCCTACACACTAATTCC
ACCTGCGAAACGCTGGTACGTTTACAGCAAATGGGAGTCGCCCGCTGGATGCTATCATCGGCGCTTACGCTGGTAATAGC
CCAGCGTCTGGTACGCAAACTTTGCCCGCATTGTCGCCGGCAGCAAGGGGAGCCCATCCATATTCCAGACAATGTATGGC
CATCGCCGCTGCCCCACTGGCAGGCACCCGGTTGTGTACATTGCTACCACGGTTTTTATGGTCGCACGGCCTTATTTGAA
GTTCTGCCCATAACACCGGTCATACGTCAGCTTATTTCCGCTAATACCGACGTTGAATCGCTGGAAACGCACGCCCGACA
GGCGGGTATGCGAACGCTTTTTGAAAACGGCTGCCTGGCCGTGGAGCAAGGCTTAACCACCTTTGAAGAGTTAATCCGCG
TATTGGGGATGCCGCATGGCGAGTAA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(88-353)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(8-68)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0H2YVM6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Glaesserella parasuis strain SC1401

41.037

100

0.412
  pilB Legionella pneumophila strain ERS1305867

49.479

83.297

0.412
  pilB Acinetobacter baylyi ADP1

39.53

100

0.401
  pilB Vibrio campbellii strain DS40M4

47.927

83.731

0.401
  pilB Vibrio cholerae strain A1552

46.154

84.599

0.39
  pilB Vibrio parahaemolyticus RIMD 2210633

45.641

84.599

0.386
  pilB Haemophilus influenzae 86-028NP

44.703

83.948

0.375
  pilB Haemophilus influenzae Rd KW20

43.928

83.948

0.369
  pilF Neisseria gonorrhoeae MS11

44.156

83.514

0.369
  pilB Acinetobacter baumannii D1279779

42.893

85.466

0.367