Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   FYA20_RS16985 Genome accession   NZ_CP043181
Coordinates   3512156..3512596 (-) Length   146 a.a.
NCBI ID   WP_000360913.1    Uniprot ID   -
Organism   Escherichia coli O2:H6 strain PG20180057     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3507156..3517596
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FYA20_RS16965 (FYA20_16965) coaE 3507646..3508266 (-) 621 WP_001269520.1 dephospho-CoA kinase -
  FYA20_RS25180 - 3508291..3508335 (+) 45 WP_120795372.1 protein YacM -
  FYA20_RS16970 (FYA20_16970) guaC 3508491..3509534 (+) 1044 WP_001217343.1 GMP reductase -
  FYA20_RS16975 (FYA20_16975) hofC 3509569..3510771 (-) 1203 WP_000157292.1 protein transport protein HofC -
  FYA20_RS16980 (FYA20_16980) pilB 3510761..3512146 (-) 1386 WP_001025178.1 type II secretion system protein GspE Machinery gene
  FYA20_RS16985 (FYA20_16985) pilA 3512156..3512596 (-) 441 WP_000360913.1 prepilin peptidase-dependent pilin Machinery gene
  FYA20_RS16990 (FYA20_16990) nadC 3512800..3513693 (-) 894 WP_001135168.1 carboxylating nicotinate-nucleotide diphosphorylase -
  FYA20_RS16995 (FYA20_16995) ampD 3513781..3514332 (+) 552 WP_000923726.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  FYA20_RS17000 (FYA20_17000) ampE 3514329..3515183 (+) 855 WP_000171984.1 beta-lactamase regulator AmpE -
  FYA20_RS17005 (FYA20_17005) aroP 3515226..3516596 (-) 1371 WP_000399017.1 aromatic amino acid transporter AroP -

Sequence

Protein


Download         Length: 146 a.a.        Molecular weight: 15634.80 Da        Isoelectric Point: 4.5237

>NTDB_id=334451 FYA20_RS16985 WP_000360913.1 3512156..3512596(-) (pilA) [Escherichia coli O2:H6 strain PG20180057]
MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQTFVPYRTAVELCALEHGGLNTCDGGSNGIPSPTTTR
YVSAMSVAKGVVSLTGQESLNGLSIVMTPGWDNANGVTGWTRNCNIQSDSALQQACEDVFRFDDAN

Nucleotide


Download         Length: 441 bp        

>NTDB_id=334451 FYA20_RS16985 WP_000360913.1 3512156..3512596(-) (pilA) [Escherichia coli O2:H6 strain PG20180057]
ATGGACAAGCAACGCGGTTTTACACTTATCGAACTGATGGTGGTTATTGGCATCATTGCCATTTTAAGCGCCATTGGTAT
TCCCGCTTATCAAAACTACCTGCGCAAAGCCGCACTCACCGACATGCTACAAACCTTTGTGCCTTACCGTACCGCCGTAG
AGTTGTGCGCGCTGGAACATGGTGGATTAAATACCTGCGACGGTGGCAGCAATGGCATTCCCTCGCCTACCACCACCCGC
TATGTTTCAGCCATGAGTGTGGCAAAGGGCGTGGTGTCGCTGACCGGGCAAGAAAGTCTCAATGGGCTAAGCATCGTCAT
GACACCGGGTTGGGATAACGCAAACGGCGTCACCGGCTGGACGCGTAACTGCAATATTCAAAGTGACAGCGCATTGCAGC
AAGCCTGCGAAGATGTCTTCCGCTTTGATGACGCCAACTAA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-25)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

43.089

84.247

0.363