Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   EO082_RS02425 Genome accession   NZ_CP034967
Coordinates   495134..496840 (-) Length   568 a.a.
NCBI ID   WP_164535952.1    Uniprot ID   -
Organism   Aeromonas veronii strain ZfB1     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 490134..501840
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EO082_RS02395 (EO082_02410) mutT 490642..491046 (+) 405 WP_042053534.1 8-oxo-dGTP diphosphatase MutT -
  EO082_RS02400 (EO082_02415) yacG 491123..491317 (-) 195 WP_005336142.1 DNA gyrase inhibitor YacG -
  EO082_RS02405 (EO082_02420) zapD 491327..492049 (-) 723 WP_005336141.1 cell division protein ZapD -
  EO082_RS02410 (EO082_02425) coaE 492087..492701 (-) 615 WP_164535950.1 dephospho-CoA kinase -
  EO082_RS02415 (EO082_02430) pilD 492715..493587 (-) 873 WP_164535951.1 prepilin peptidase Machinery gene
  EO082_RS02420 (EO082_02435) pilC 493649..494890 (-) 1242 WP_005336136.1 type II secretion system F family protein Machinery gene
  EO082_RS02425 (EO082_02440) pilB 495134..496840 (-) 1707 WP_164535952.1 PilB family type IVa pilus assembly ATPase TapB Machinery gene
  EO082_RS02430 (EO082_02445) - 496844..497248 (-) 405 WP_272953589.1 pilin -
  EO082_RS02435 (EO082_02455) nadC 497564..498427 (-) 864 WP_164535953.1 carboxylating nicotinate-nucleotide diphosphorylase -
  EO082_RS02440 (EO082_02460) - 498431..498901 (-) 471 WP_164535954.1 retropepsin-like aspartic protease family protein -
  EO082_RS02445 (EO082_02465) ampD 499032..499607 (+) 576 WP_164535955.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  EO082_RS02450 (EO082_02470) pdhR 499886..500650 (+) 765 WP_005336127.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -

Sequence

Protein


Download         Length: 568 a.a.        Molecular weight: 62541.92 Da        Isoelectric Point: 5.3192

>NTDB_id=333760 EO082_RS02425 WP_164535952.1 495134..496840(-) (pilB) [Aeromonas veronii strain ZfB1]
MTSSPNSGLALSLAASSLISEEDSQRYLIQAKAQRKPFVTFLIENEILDSKALADFCELEYGVPLLDLAAFDLAEIPQKY
LNQKLIEKHHVLPIYTQGHTLYIAMSDPTNVSALEDFGFSFGLHTEALLVEENKLTAAIGKLLESENDALGMEDIDESEI
SDLEVSDENSRLDESVNTADDDAPIVKYINKILMDAIKRGASDLHFEPYEFKYRVRFRIDGILHEIATPPVNLSNRFSAR
LKVMARLDIAERRLPQDGRIKLKLSRNKSIDMRVNTLPTMWGEKIVIRLLDSSAARLNIEQLGFDDRQKAQYLRALSKPQ
GMILVTGPTGSGKTVSLYTGLNILNTTEVNISTAEDPVEINLPGVNQVQVNPKAGLTFASALRSFLRQDPDVVMVGEIRD
LETAEIAIKAAQTGHLVLSTLHTNSAAETLTRMMNMGVPAFNIASSVTLIMAQRLARKLCDHCKAPEVVPEAELLELGFT
PQQLAAGLRLFKPVGCKECSGGYKGRVGIYEIMLMSENIAKLIMQGANSLQIAAIAQKEGMRTLRTSGLEKARLGVTSLA
EVNRVTTN

Nucleotide


Download         Length: 1707 bp        

>NTDB_id=333760 EO082_RS02425 WP_164535952.1 495134..496840(-) (pilB) [Aeromonas veronii strain ZfB1]
ATGACCTCTAGCCCTAATAGCGGCCTGGCGTTAAGCCTGGCCGCCTCTTCACTGATCAGTGAAGAGGACTCCCAGCGCTA
TCTGATCCAGGCCAAGGCTCAGCGCAAACCCTTTGTGACATTTCTGATAGAGAACGAGATCCTCGACAGCAAGGCGCTCG
CCGATTTTTGCGAGCTGGAGTATGGGGTACCTCTGCTGGATCTTGCCGCCTTCGACTTGGCCGAGATCCCGCAGAAGTAC
CTCAATCAGAAGCTGATTGAGAAACACCATGTGCTGCCCATCTACACCCAGGGCCACACCCTCTATATCGCCATGTCGGA
TCCGACCAATGTCTCGGCGTTGGAGGACTTTGGCTTCAGCTTCGGTCTTCATACTGAAGCCCTGCTGGTGGAGGAGAACA
AGCTCACCGCCGCCATCGGCAAGCTGCTGGAGAGCGAAAATGACGCTCTCGGCATGGAGGATATCGACGAGTCCGAGATT
TCGGATCTCGAAGTCTCCGACGAAAACAGCCGACTGGACGAAAGCGTCAATACCGCCGATGACGATGCTCCCATCGTCAA
GTACATCAACAAGATCCTGATGGATGCCATCAAGCGCGGCGCCTCCGACCTGCACTTCGAGCCCTATGAGTTCAAGTATC
GGGTGCGCTTTCGCATCGACGGCATTCTGCATGAAATCGCTACACCGCCGGTCAACCTCTCCAACCGCTTTTCCGCCCGC
CTCAAGGTCATGGCACGACTCGATATCGCCGAGCGGCGGCTGCCACAGGATGGCCGCATCAAGTTGAAGTTGTCGCGCAA
TAAGTCGATTGATATGCGGGTCAATACCCTGCCCACCATGTGGGGAGAGAAGATCGTTATCCGTCTGCTCGACTCCTCGG
CGGCACGCCTCAATATCGAGCAACTCGGCTTTGATGACCGACAGAAGGCGCAGTACCTGCGCGCCCTCTCCAAGCCACAG
GGGATGATCCTGGTGACCGGCCCCACCGGCTCGGGCAAGACGGTTTCACTCTATACCGGCCTCAATATTCTCAACACCAC
CGAGGTAAATATCTCTACCGCCGAAGATCCGGTGGAAATCAACCTGCCCGGCGTCAATCAGGTGCAAGTCAACCCCAAGG
CGGGGCTCACCTTTGCCAGCGCCCTGCGCTCCTTCTTGCGACAAGACCCGGATGTGGTGATGGTGGGGGAAATTCGCGAT
CTCGAAACCGCCGAAATCGCTATCAAGGCGGCCCAGACCGGTCACCTGGTGCTATCCACCCTGCACACAAACTCCGCCGC
CGAGACTCTGACCCGGATGATGAACATGGGGGTACCGGCCTTCAACATCGCCTCCTCGGTGACCCTGATCATGGCCCAGC
GGTTGGCCCGTAAACTGTGCGACCACTGCAAAGCGCCGGAAGTGGTGCCGGAAGCCGAACTGCTGGAGCTGGGCTTTACG
CCGCAGCAGCTGGCAGCCGGACTGCGACTGTTCAAACCGGTCGGCTGCAAGGAGTGTTCCGGCGGCTACAAGGGGCGGGT
CGGCATATACGAAATCATGCTGATGTCGGAGAACATCGCCAAATTAATCATGCAGGGGGCCAACTCCCTGCAAATCGCTG
CTATCGCGCAGAAAGAGGGGATGCGTACCTTACGTACCTCGGGACTGGAGAAGGCTCGGCTCGGGGTCACCAGTCTGGCC
GAGGTCAATCGGGTCACCACCAACTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(191-458)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-146)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

55.965

100

0.562
  pilB Acinetobacter baumannii D1279779

56.162

100

0.562
  pilB Legionella pneumophila strain ERS1305867

56.827

95.423

0.542
  pilB Vibrio cholerae strain A1552

54.937

98.063

0.539
  pilB Vibrio campbellii strain DS40M4

51.394

100

0.519
  pilB Vibrio parahaemolyticus RIMD 2210633

51.773

99.296

0.514
  pilF Neisseria gonorrhoeae MS11

46.809

99.296

0.465
  pilB/pilB1 Synechocystis sp. PCC 6803

39.06

97.359

0.38
  pilF Thermus thermophilus HB27

40.53

92.958

0.377

Multiple sequence alignment