Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   ELZ14_RS02375 Genome accession   NZ_CP034725
Coordinates   516106..518196 (+) Length   696 a.a.
NCBI ID   WP_003196981.1    Uniprot ID   -
Organism   Pseudomonas brassicacearum strain 3Re2-7     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 511106..523196
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ELZ14_RS02355 (ELZ14_02355) comM 513318..514382 (+) 1065 WP_003196973.1 pilus assembly protein PilM Machinery gene
  ELZ14_RS02360 (ELZ14_02360) - 514382..514948 (+) 567 WP_003196975.1 PilN domain-containing protein -
  ELZ14_RS02365 (ELZ14_02365) pilO 514945..515568 (+) 624 WP_003196977.1 type 4a pilus biogenesis protein PilO -
  ELZ14_RS02370 (ELZ14_02370) - 515565..516092 (+) 528 WP_003196979.1 pilus assembly protein PilP -
  ELZ14_RS02375 (ELZ14_02375) pilQ 516106..518196 (+) 2091 WP_003196981.1 type IV pilus secretin PilQ Machinery gene
  ELZ14_RS02380 (ELZ14_02380) aroK 518201..518719 (+) 519 WP_003196983.1 shikimate kinase AroK -
  ELZ14_RS02385 (ELZ14_02385) aroB 518868..519968 (+) 1101 WP_003196985.1 3-dehydroquinate synthase -
  ELZ14_RS02390 (ELZ14_02390) - 519978..521558 (+) 1581 WP_003196987.1 AAA family ATPase -

Sequence

Protein


Download         Length: 696 a.a.        Molecular weight: 75084.12 Da        Isoelectric Point: 5.2961

>NTDB_id=332757 ELZ14_RS02375 WP_003196981.1 516106..518196(+) (pilQ) [Pseudomonas brassicacearum strain 3Re2-7]
MNRFFSALGISLWIALLSPMVLGASLKTLDVAALPGDRVELKLSFDGPPPSPRGYTTEQPARIALDLPGVTNQLSSKRRD
LGGGNARSATVVEAGDRTRLIIGLTQLAPYDTRIEGNNLFVVVGKGAAPKSTAPKNSTTQAPRPAVASAAPARASAPVGK
AIRGVDFQRGTQGEGNVVIDLSDPSIAPDIQEREGKIILNFARTQLPEPLRVRLDVKDFATPVQFVNASAGSDRATISIE
PSGTFDYSTYQTDNKLTVSVRPMTVDDLQKRNADRAAYSGEKLSLNFQDIEVRSVLQLIADFTNLNLVASDTVQGGITLR
LQNVPWDQALDLVLKTKGLDKRKVGNVLLVAPADEIAARERQELESQKQIADLAPLRRELLQVNYAKAADIAKLFQSVTS
AEAKADERGTITVDERTNNIIAYQTQDRLDELRRIVAQLDIPVRQVMIEARIVEANVDYDKSLGVRWGGSVQNKGNWSSS
GVTTGDSTTVGTPGSTSTNQPFVDLGASGNTSGIGIAFITDNVLLDLELTAMEKTGNGEIVSQPKVVTSDKETAKILKGT
EIPYQEASSSGATSVSFKEASLSLEVTPQITPDNRIIMEVKVTKDEPDYLNKVNDVPPIKKNEVNAKVLVNDGETIVIGG
VFSNTQSKVVDKVPFLGDVPYLGRLFRRDVVSDSKSELLVFLTPRIMNNQAIAVSR

Nucleotide


Download         Length: 2091 bp        

>NTDB_id=332757 ELZ14_RS02375 WP_003196981.1 516106..518196(+) (pilQ) [Pseudomonas brassicacearum strain 3Re2-7]
ATGAACAGGTTTTTTTCAGCCCTCGGTATTTCGCTATGGATAGCGCTGTTGTCGCCGATGGTTCTCGGGGCCAGCCTGAA
GACGTTGGATGTGGCGGCGTTGCCGGGGGACCGTGTCGAGTTGAAGCTCTCCTTCGACGGGCCGCCGCCGTCACCCCGGG
GCTACACCACCGAACAACCGGCGCGGATTGCGCTGGACCTGCCGGGCGTGACCAATCAACTTTCCAGCAAGCGCCGCGAC
CTGGGCGGGGGCAATGCACGCAGCGCCACTGTGGTCGAGGCTGGGGATCGTACTCGGTTGATCATCGGCCTTACCCAGCT
GGCGCCGTATGACACCCGGATCGAAGGTAACAACCTGTTCGTGGTGGTTGGCAAGGGCGCCGCCCCGAAGAGCACTGCCC
CGAAGAACTCCACCACCCAGGCCCCGCGCCCGGCGGTTGCCAGCGCTGCGCCTGCGAGGGCCAGTGCGCCGGTGGGCAAG
GCGATCCGTGGGGTGGACTTCCAGCGCGGGACCCAGGGCGAAGGCAACGTGGTGATCGACCTGTCTGATCCGTCCATCGC
CCCGGACATCCAGGAGCGCGAAGGCAAGATCATCCTCAATTTCGCCAGGACCCAGTTGCCCGAGCCGCTGCGGGTGCGCC
TGGACGTCAAGGACTTCGCCACCCCGGTGCAATTCGTCAATGCCAGCGCCGGCAGCGACCGGGCGACCATCAGCATCGAA
CCCAGCGGGACTTTCGACTACTCGACCTACCAGACCGACAACAAGCTCACCGTCAGCGTCCGGCCCATGACCGTCGACGA
CCTGCAAAAACGCAACGCCGACCGCGCGGCCTACAGTGGCGAGAAGCTCTCGCTCAACTTCCAGGACATCGAGGTGCGTT
CGGTCCTGCAACTGATTGCCGATTTTACCAACCTGAACCTCGTGGCCAGCGACACGGTGCAGGGTGGCATCACCCTGCGG
TTGCAGAATGTGCCCTGGGACCAGGCGCTCGACCTGGTGCTCAAGACCAAGGGGTTGGACAAGCGCAAGGTCGGCAACGT
GCTGTTGGTGGCTCCGGCCGATGAAATTGCCGCTCGTGAGCGCCAGGAACTGGAGTCGCAGAAGCAGATTGCCGACCTGG
CACCGCTGCGGCGTGAACTGTTGCAGGTCAACTATGCGAAGGCGGCCGACATCGCCAAGCTGTTCCAGTCGGTGACCAGT
GCCGAGGCCAAAGCCGATGAGCGCGGCACGATCACGGTCGATGAGCGAACCAACAACATCATTGCCTACCAGACCCAGGA
CCGCCTCGACGAACTGCGCCGGATCGTGGCGCAACTGGATATTCCGGTGCGCCAGGTGATGATCGAGGCGCGGATTGTCG
AAGCCAATGTCGACTACGACAAAAGCCTGGGCGTGCGCTGGGGCGGTTCGGTTCAAAACAAGGGCAACTGGAGCTCCTCG
GGGGTTACCACGGGCGATTCCACCACCGTTGGCACGCCGGGTAGCACCAGCACCAACCAGCCGTTCGTCGACCTGGGCGC
CTCGGGCAATACCTCGGGCATCGGCATTGCCTTCATCACCGACAACGTATTGCTGGACCTTGAGCTGACGGCCATGGAGA
AAACCGGCAACGGGGAGATCGTCTCCCAGCCCAAGGTGGTGACGTCCGACAAGGAGACGGCAAAGATCCTCAAGGGCACG
GAAATTCCTTATCAGGAAGCCAGTTCCAGCGGCGCTACGTCGGTGTCGTTCAAGGAGGCTTCGCTGTCGCTGGAGGTGAC
CCCGCAGATCACTCCAGACAACCGGATCATCATGGAGGTCAAGGTCACCAAGGATGAGCCGGACTACCTGAACAAGGTGA
ATGATGTACCGCCGATCAAGAAAAACGAGGTCAATGCCAAGGTCCTGGTCAATGATGGCGAGACCATTGTGATTGGCGGG
GTTTTCTCAAATACTCAGAGCAAGGTTGTAGATAAGGTGCCATTTCTTGGCGATGTGCCGTATCTTGGCCGCCTTTTCCG
GCGTGATGTGGTTTCGGATAGCAAATCCGAGCTGCTGGTGTTCCTCACTCCGCGTATCATGAATAACCAGGCGATTGCTG
TGAGTCGTTGA

Domains

Predicted by InterproScan.

Bacterial type II/III secretion system short domain (Secretin_N)

(380-445)

Secretin and TonB N terminus short domain (STN)

(306-352)

Bacterial type II and III secretion system protein (Secretin)

(531-687)

AMIN domain (AMIN)

(37-121)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

73.427

100

0.754
  pilQ Acinetobacter baumannii strain A118

38.71

100

0.414
  pilQ Acinetobacter baumannii D1279779

38.71

100

0.414
  comQ Acinetobacter baylyi ADP1

40.948

100

0.409
  pilQ Legionella pneumophila strain Lp02

38.179

97.845

0.374
  pilQ Legionella pneumophila strain ERS1305867

38.201

97.414

0.372

Multiple sequence alignment