Detailed information    

insolico Bioinformatically predicted

Overview

Name   comM   Type   Machinery gene
Locus tag   ELZ14_RS02355 Genome accession   NZ_CP034725
Coordinates   513318..514382 (+) Length   354 a.a.
NCBI ID   WP_003196973.1    Uniprot ID   A0A0Q7AAJ5
Organism   Pseudomonas brassicacearum strain 3Re2-7     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 508318..519382
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ELZ14_RS02340 (ELZ14_02340) - 509036..510304 (+) 1269 WP_003196970.1 malic enzyme-like NAD(P)-binding protein -
  ELZ14_RS02350 (ELZ14_02350) - 510665..513109 (-) 2445 WP_377589109.1 penicillin-binding protein 1A -
  ELZ14_RS02355 (ELZ14_02355) comM 513318..514382 (+) 1065 WP_003196973.1 pilus assembly protein PilM Machinery gene
  ELZ14_RS02360 (ELZ14_02360) - 514382..514948 (+) 567 WP_003196975.1 PilN domain-containing protein -
  ELZ14_RS02365 (ELZ14_02365) pilO 514945..515568 (+) 624 WP_003196977.1 type 4a pilus biogenesis protein PilO -
  ELZ14_RS02370 (ELZ14_02370) - 515565..516092 (+) 528 WP_003196979.1 pilus assembly protein PilP -
  ELZ14_RS02375 (ELZ14_02375) pilQ 516106..518196 (+) 2091 WP_003196981.1 type IV pilus secretin PilQ Machinery gene
  ELZ14_RS02380 (ELZ14_02380) aroK 518201..518719 (+) 519 WP_003196983.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 354 a.a.        Molecular weight: 38084.64 Da        Isoelectric Point: 4.9070

>NTDB_id=332756 ELZ14_RS02355 WP_003196973.1 513318..514382(+) (comM) [Pseudomonas brassicacearum strain 3Re2-7]
MLRLFNKKAHALLGIDISSTSVKLLELSRQGDRYRVESYAVEPLPANAVVEKNIAELEGVGQALSRLLAKAKTPLRSVAV
AVAGSAVITKTIEMDAGLSDDDMENQLKIEADQYIPYPLDEVAIDFEVMGVSPRSAERVEVLLAACRKENVEVREAALAL
AGLTARVVDVEAYALERAFGLLATQLAASQERLTVAVVDIGATMTTLSVLHNGRIIYTREQLFGGRQLTEEIQRRYGLTP
EQAGQAKKQGGLPDDYLSEVLQPFREALVQQVSRSLQFFFASGQYSAVDHILLAGGTASVAGLDRLIEQRLGTPTQVANP
FTNMALSSKVNAGALAGDAPALMIACGLALRSFD

Nucleotide


Download         Length: 1065 bp        

>NTDB_id=332756 ELZ14_RS02355 WP_003196973.1 513318..514382(+) (comM) [Pseudomonas brassicacearum strain 3Re2-7]
GTGCTACGACTCTTCAATAAAAAAGCCCATGCGCTTCTGGGGATCGACATCAGTTCGACGTCGGTGAAGCTGCTGGAGTT
GAGCCGCCAGGGCGATCGATACCGCGTCGAGTCCTACGCGGTCGAACCGTTGCCAGCCAACGCTGTGGTCGAGAAGAACA
TCGCCGAGCTCGAAGGGGTGGGGCAGGCGTTGTCGCGCCTGTTGGCCAAGGCCAAGACGCCCTTGCGTAGCGTGGCGGTG
GCGGTGGCGGGTTCAGCGGTGATCACCAAGACCATCGAGATGGACGCCGGCCTGTCCGACGATGACATGGAAAACCAGCT
CAAGATCGAGGCCGATCAATACATCCCCTATCCCCTGGATGAGGTGGCCATCGATTTCGAAGTGATGGGTGTTTCGCCAC
GCAGTGCCGAGCGCGTCGAAGTGCTGCTGGCGGCGTGCCGCAAGGAAAACGTCGAGGTCCGCGAGGCCGCATTGGCGCTC
GCCGGGCTGACGGCCCGGGTGGTCGATGTGGAGGCTTATGCGCTGGAGCGTGCTTTTGGCCTGCTCGCCACGCAACTGGC
TGCCTCCCAGGAACGGCTGACTGTGGCGGTCGTCGATATCGGCGCCACCATGACCACCCTCAGCGTGCTGCACAACGGGC
GCATCATCTATACCCGCGAACAATTGTTCGGCGGCCGCCAACTGACCGAGGAGATCCAGCGCCGCTATGGCCTGACACCC
GAGCAGGCTGGCCAGGCAAAGAAGCAGGGTGGCCTGCCGGACGACTATCTCAGCGAAGTGCTGCAACCTTTTCGCGAGGC
TCTGGTACAGCAGGTCTCGCGGTCCTTGCAGTTCTTCTTCGCCTCGGGCCAGTACAGTGCGGTGGACCACATTCTGCTGG
CCGGGGGCACGGCTTCGGTCGCCGGCCTGGATCGGCTGATCGAGCAGCGCCTGGGCACGCCGACCCAGGTGGCCAACCCG
TTTACCAACATGGCCCTGAGCAGCAAGGTCAATGCCGGCGCCCTGGCCGGTGATGCGCCGGCACTGATGATTGCCTGTGG
GCTGGCCCTCAGGAGTTTTGACTGA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(14-353)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0Q7AAJ5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comM Acinetobacter nosocomialis M2

55.932

100

0.559
  pilM Acinetobacter baumannii D1279779

55.932

100

0.559
  comM Acinetobacter baylyi ADP1

54.802

100

0.548
  pilM Legionella pneumophila strain ERS1305867

47.458

100

0.475

Multiple sequence alignment