Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   ELR50_RS20295 Genome accession   NZ_CP034688
Coordinates   4543247..4545340 (+) Length   697 a.a.
NCBI ID   WP_064584719.1    Uniprot ID   A0A1A9KK03
Organism   Pseudomonas citronellolis strain WXP-4     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4538247..4550340
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ELR50_RS20275 (ELR50_20200) pilM 4540353..4541417 (+) 1065 WP_009613650.1 pilus assembly protein PilM Machinery gene
  ELR50_RS20280 (ELR50_20205) pilN 4541417..4542016 (+) 600 WP_009613648.1 type 4a pilus biogenesis protein PilN -
  ELR50_RS20285 (ELR50_20210) pilO 4542013..4542669 (+) 657 WP_009613645.1 type 4a pilus biogenesis protein PilO -
  ELR50_RS20290 (ELR50_20215) pilP 4542666..4543190 (+) 525 WP_009613643.1 type 4a pilus biogenesis lipoprotein PilP -
  ELR50_RS20295 (ELR50_20220) pilQ 4543247..4545340 (+) 2094 WP_064584719.1 type IV pilus secretin PilQ Machinery gene
  ELR50_RS20300 (ELR50_20225) aroK 4545376..4545867 (+) 492 WP_043268468.1 shikimate kinase AroK -
  ELR50_RS20305 (ELR50_20230) aroB 4545919..4547025 (+) 1107 WP_009613636.1 3-dehydroquinate synthase -
  ELR50_RS20310 (ELR50_20235) - 4547032..4548708 (+) 1677 WP_043268470.1 AAA family ATPase -

Sequence

Protein


Download         Length: 697 a.a.        Molecular weight: 74897.03 Da        Isoelectric Point: 5.2731

>NTDB_id=332554 ELR50_RS20295 WP_064584719.1 4543247..4545340(+) (pilQ) [Pseudomonas citronellolis strain WXP-4]
MNRTMTRLGFALLAGLFAPSLLAANLQRVDVASLPGDRVELKLGFDEPVAAPRGYTIEQPARIALDLPGVQNKLGSKNRE
LGVGNARSLTVVEAKDRTRLIINLTTLVPYSTRAQGNDLIILVGQGGGAPAAAASSAPSAAPAYAAAPRPAQPKPYVPVG
KAIRNIDFQRGEQGEGNVVIDLSDPSVSPDIQEQGGKIRLDFPRTQLPDSLRVRLDVKDFATPVQFVNASAAGDKASIVI
EPSGLYDYLVFQTDNRMTVSIKPMTANEAEKRKKDSFAYTGEKLSLNFQDIDVRSVLQLIADFTDLNLVASDTVQGNITL
RLQNVPWDQALDLVLKTKGLDKRKVGNVLLVAPADEIAARERQELEAQKQIAELAPLRRELIQVNYAKASDIAKLFQSVT
GTSSGPGAQPAAGTDNRGSITVDDRTNAIIAYQTQDRLDELRRIVSQLDIPVRQVMIEARIVEASVDYTKELGVQWGGSL
SNRGNFNITGGTGGTSSANTFVDLGVSTASPTSGIGIGFVTDNTTLDLQLNAMEKTGNGEVVSQPKVVTSDKETAKILKG
SEIPYQEASSSGATSVSFKEAALSLEVTPQITPDNRIIMQVKVNKDEPDYQNALLGVPPIKKNEVNAKVLVNDGETIVIG
GVFSNTQTKTTSKVPFLGDVPVVGRLFKNDYVTDTKNELLVFLTPRIMNNQAIAIDR

Nucleotide


Download         Length: 2094 bp        

>NTDB_id=332554 ELR50_RS20295 WP_064584719.1 4543247..4545340(+) (pilQ) [Pseudomonas citronellolis strain WXP-4]
ATGAACAGAACTATGACCCGCCTGGGCTTTGCCCTGTTGGCCGGCCTTTTCGCGCCGAGCCTGCTCGCCGCCAACCTGCA
GCGCGTCGACGTGGCCTCGCTGCCGGGGGATCGCGTGGAGCTCAAGCTGGGCTTCGACGAGCCGGTGGCCGCGCCGCGTG
GCTACACCATCGAGCAGCCGGCGCGCATCGCCCTGGACCTGCCGGGCGTGCAGAACAAGCTGGGCAGCAAGAACCGCGAG
CTGGGCGTGGGCAACGCGCGCAGCCTGACCGTGGTCGAGGCCAAGGACCGCACCCGCCTGATCATCAACCTGACCACCCT
GGTGCCGTATTCCACCCGCGCCCAGGGCAACGACCTGATCATCCTGGTCGGCCAGGGTGGCGGCGCGCCCGCCGCGGCGG
CCAGCTCCGCGCCGAGCGCGGCCCCGGCCTATGCCGCGGCGCCTCGCCCGGCGCAGCCCAAGCCCTACGTGCCGGTGGGC
AAGGCGATCCGCAACATCGACTTCCAGCGCGGCGAGCAGGGCGAGGGCAACGTGGTCATCGACCTGTCCGACCCGAGCGT
CAGCCCGGACATCCAGGAGCAGGGCGGCAAGATCCGCCTGGACTTCCCGCGCACCCAGCTGCCCGACAGCCTGCGCGTGC
GCCTGGACGTGAAGGACTTCGCCACCCCGGTGCAGTTCGTCAACGCCTCGGCGGCCGGCGACAAGGCCAGCATCGTCATC
GAGCCCAGCGGCCTGTACGACTACCTGGTGTTCCAGACCGACAACCGCATGACCGTCAGCATCAAGCCGATGACCGCCAA
CGAGGCCGAGAAGCGCAAGAAGGACAGCTTCGCCTACACCGGCGAGAAGCTGTCGCTGAACTTCCAGGACATCGACGTGC
GCTCGGTGCTGCAGCTGATCGCCGACTTCACCGACCTCAACCTGGTGGCCTCGGACACCGTGCAGGGCAACATCACCCTG
CGCCTGCAGAACGTGCCCTGGGACCAGGCGCTGGACCTGGTGCTCAAGACCAAGGGCCTGGACAAGCGCAAGGTCGGCAA
CGTCCTGCTGGTGGCGCCGGCCGACGAGATCGCCGCGCGCGAGCGCCAGGAGCTGGAAGCGCAGAAGCAGATCGCCGAAC
TGGCGCCCCTGCGTCGCGAACTGATCCAGGTGAACTACGCCAAGGCTTCCGACATCGCCAAGCTGTTCCAGTCCGTGACC
GGCACCAGTTCCGGCCCGGGCGCCCAGCCTGCCGCCGGGACCGACAACCGTGGCTCCATCACCGTCGACGACCGCACCAA
CGCCATCATCGCCTACCAGACCCAGGACCGCCTGGACGAGCTGCGCCGCATCGTCTCGCAGCTGGACATCCCGGTACGCC
AGGTGATGATCGAGGCGCGCATCGTCGAGGCCAGCGTCGACTACACCAAGGAGCTGGGGGTGCAATGGGGTGGTTCGCTG
AGCAACCGTGGCAACTTCAATATCACCGGTGGTACTGGTGGTACCAGCAGTGCGAATACCTTCGTCGATCTGGGCGTCAG
CACCGCGTCCCCGACCTCCGGCATCGGCATCGGCTTCGTCACCGACAACACCACCCTGGACCTGCAGCTCAACGCCATGG
AGAAGACCGGCAACGGCGAGGTGGTGTCGCAGCCGAAGGTGGTCACCTCGGACAAGGAAACCGCGAAGATCCTCAAGGGC
TCGGAGATTCCCTACCAGGAAGCCAGCTCCAGCGGCGCCACCTCGGTGTCCTTCAAGGAGGCCGCGCTGTCCCTGGAGGT
CACCCCGCAGATCACCCCGGACAACCGCATCATCATGCAGGTCAAGGTGAACAAGGACGAGCCCGACTACCAGAACGCGC
TGCTCGGCGTGCCGCCGATCAAGAAGAACGAGGTCAACGCCAAGGTGCTGGTCAACGACGGTGAAACCATCGTCATCGGC
GGGGTGTTCTCCAACACCCAGACCAAGACCACCTCCAAGGTCCCGTTCCTCGGCGACGTACCGGTCGTCGGCCGCCTGTT
CAAGAACGACTATGTGACGGACACCAAGAACGAGCTTCTGGTTTTCCTCACTCCCCGGATCATGAATAATCAGGCCATTG
CCATCGATCGTTGA

Domains

Predicted by InterproScan.

AMIN domain (AMIN)

(40-121)

Secretin and TonB N terminus short domain (STN)

(307-353)

Bacterial type II and III secretion system protein (Secretin)

(532-688)

Bacterial type II/III secretion system short domain (Secretin_N)

(381-454)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1A9KK03

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

77.178

100

0.801
  comQ Acinetobacter baylyi ADP1

41.667

99.857

0.416
  pilQ Acinetobacter baumannii strain A118

40.673

100

0.416
  pilQ Acinetobacter baumannii D1279779

40.673

100

0.416
  pilQ Legionella pneumophila strain Lp02

39.385

97.991

0.386
  pilQ Legionella pneumophila strain ERS1305867

39.239

97.991

0.385

Multiple sequence alignment