Detailed information NTG0332553

Bioinformatically predicted

Overview

• Name: pilM
• Type: Machinery gene
• Locus tag: ELR50_RS20275
• Genome accession: NZ_CP034688
• Coordinates: 4540353..4541417 (+)
• Length: 354 a.a.
• NCBI ID: WP_009613650.1
• Uniprot ID: A0A1A5D9E0
• Organism: Pseudomonas citronellolis strain WXP-4
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 4535353..4546417

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ELR50_RS20255 (ELR50_20180)	-	4535441..4535863 (+)	423	WP_253391052.1	hypothetical protein	-
ELR50_RS20260 (ELR50_20185)	-	4535906..4536676 (+)	771	WP_269962151.1	class I SAM-dependent methyltransferase	-
ELR50_RS20265 (ELR50_20190)	-	4536738..4537229 (+)	492	WP_043268464.1	M48 family metallopeptidase	-
ELR50_RS20270 (ELR50_20195)	-	4537735..4540167 (-)	2433	WP_269962152.1	penicillin-binding protein 1A	-
ELR50_RS20275 (ELR50_20200)	pilM	4540353..4541417 (+)	1065	WP_009613650.1	pilus assembly protein PilM	Machinery gene
ELR50_RS20280 (ELR50_20205)	pilN	4541417..4542016 (+)	600	WP_009613648.1	type 4a pilus biogenesis protein PilN	-
ELR50_RS20285 (ELR50_20210)	pilO	4542013..4542669 (+)	657	WP_009613645.1	type 4a pilus biogenesis protein PilO	-
ELR50_RS20290 (ELR50_20215)	pilP	4542666..4543190 (+)	525	WP_009613643.1	type 4a pilus biogenesis lipoprotein PilP	-
ELR50_RS20295 (ELR50_20220)	pilQ	4543247..4545340 (+)	2094	WP_064584719.1	type IV pilus secretin PilQ	Machinery gene
ELR50_RS20300 (ELR50_20225)	aroK	4545376..4545867 (+)	492	WP_043268468.1	shikimate kinase AroK	-

Sequence

Protein

• Length: 354 a.a.
• Molecular weight: 37773.09 Da
• Isoelectric Point: 4.4526

>NTDB_id=332553 ELR50_RS20275 WP_009613650.1 4540353..4541417(+) (pilM) [Pseudomonas citronellolis strain WXP-4]
MLGLFTKKANTLLGIDISSTSVKLLELSRSGGRYKVEAYAVEPLPPNSVVEKNIAELEGVGQALSRVVAKARTGVKTAAV
AVAGSAVITKTIEMDGGLSEDELENQLKIEADQYIPYPLEEVAIDFEVQGVSARNAERVDVLLAACRKENVEVREAALAL
AGLTAKVVDVEAYALERAFGLLGDQLGGNADELTVAVVDIGATMTTLSVLHHGRTIYTREQLFGGRQLTEEIQRRYGLSV
EEAGLAKKQGGLPDDYDSEVLQPFKDAVVQQVSRSLQFFFAAGQFNDVDYILLAGGTASIPDLDRLIQQKIGTPTLVANP
FADMVLSNKVNAGALASDAPALMIACGLAMRSFD

Nucleotide

• Length: 1065 bp

>NTDB_id=332553 ELR50_RS20275 WP_009613650.1 4540353..4541417(+) (pilM) [Pseudomonas citronellolis strain WXP-4]
GTGCTAGGGCTCTTCACTAAGAAAGCGAACACACTGCTGGGGATCGACATCAGTTCGACTTCGGTCAAACTCCTCGAGTT
GAGCCGTTCGGGCGGGCGCTACAAGGTAGAGGCCTACGCCGTAGAGCCGCTCCCGCCGAACTCGGTGGTCGAGAAAAACA
TCGCCGAACTGGAGGGGGTCGGCCAGGCGCTGTCGCGGGTGGTCGCCAAGGCGCGCACCGGCGTGAAGACGGCTGCCGTG
GCGGTCGCCGGCTCGGCGGTCATCACCAAGACCATCGAGATGGACGGCGGGCTTTCCGAAGACGAGCTGGAGAACCAGCT
CAAGATCGAAGCCGACCAGTACATTCCCTATCCCCTGGAGGAAGTCGCCATCGACTTCGAGGTCCAGGGCGTATCCGCGC
GCAACGCCGAACGCGTCGACGTGCTGCTGGCGGCCTGCCGCAAGGAGAACGTCGAGGTGCGCGAGGCCGCGCTGGCCCTG
GCCGGGCTCACCGCCAAGGTGGTGGACGTCGAGGCCTACGCGCTGGAGCGCGCCTTCGGCCTGCTCGGCGACCAGCTGGG
CGGCAACGCCGACGAGCTGACCGTCGCGGTGGTCGACATCGGCGCCACCATGACCACCCTCAGCGTCCTCCACCACGGCC
GCACCATCTATACCCGCGAGCAACTGTTCGGCGGCCGCCAGCTCACCGAGGAGATCCAGCGCCGCTATGGCCTGTCGGTG
GAAGAAGCCGGCCTCGCCAAGAAGCAGGGCGGTCTTCCGGATGACTACGACAGCGAAGTGCTGCAACCGTTCAAGGATGC
GGTGGTGCAGCAGGTCTCGCGTTCGCTGCAGTTCTTCTTCGCCGCTGGCCAGTTCAACGACGTCGACTACATCCTGCTCG
CCGGCGGCACGGCTTCCATCCCCGACCTGGACCGGCTCATCCAGCAGAAGATCGGCACCCCGACGCTGGTGGCCAACCCC
TTCGCCGACATGGTGCTGAGCAACAAGGTGAATGCCGGGGCGCTGGCCAGTGACGCGCCGGCGCTGATGATTGCCTGCGG
TCTGGCGATGAGGAGTTTCGACTGA

Domains

Predicted by InterproScan.

• Type IV pilus assembly protein PilM; (PilM_2) (14-353)

3D structure

• AlphaFold DB: A0A1A5D9E0

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilM	Acinetobacter baumannii D1279779	57.345	100	0.573
comM	Acinetobacter nosocomialis M2	57.345	100	0.573
comM	Acinetobacter baylyi ADP1	53.39	100	0.534
pilM	Legionella pneumophila strain ERS1305867	46.045	100	0.46