Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   DYL72_RS10350 Genome accession   NZ_CP034672
Coordinates   2149948..2151636 (-) Length   562 a.a.
NCBI ID   WP_029388146.1    Uniprot ID   A0A289G7S6
Organism   Vibrio anguillarum strain J360     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2144948..2156636
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DYL72_RS10320 (DYL72_10320) rplS 2145677..2146030 (+) 354 WP_010319525.1 50S ribosomal protein L19 -
  DYL72_RS10325 (DYL72_10325) yacG 2146100..2146297 (-) 198 WP_010319526.1 DNA gyrase inhibitor YacG -
  DYL72_RS10330 (DYL72_10330) zapD 2146387..2147127 (-) 741 WP_019281903.1 cell division protein ZapD -
  DYL72_RS10335 (DYL72_10335) coaE 2147160..2147768 (-) 609 WP_013857688.1 dephospho-CoA kinase -
  DYL72_RS10340 (DYL72_10340) pilD 2147759..2148640 (-) 882 WP_019281902.1 prepilin peptidase Machinery gene
  DYL72_RS10345 (DYL72_10345) pilC 2148679..2149905 (-) 1227 WP_069212069.1 type II secretion system F family protein Machinery gene
  DYL72_RS10350 (DYL72_10350) pilB 2149948..2151636 (-) 1689 WP_029388146.1 type IV-A pilus assembly ATPase PilB Machinery gene
  DYL72_RS10355 (DYL72_10355) - 2151642..2152076 (-) 435 WP_050934217.1 type IV pilin protein -
  DYL72_RS10360 (DYL72_10360) nadC 2152332..2153219 (-) 888 WP_019281900.1 carboxylating nicotinate-nucleotide diphosphorylase -
  DYL72_RS10365 (DYL72_10365) ampD 2153344..2153895 (+) 552 WP_214650766.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  DYL72_RS10370 (DYL72_10370) fldB 2153917..2154435 (-) 519 WP_013857680.1 flavodoxin FldB -
  DYL72_RS10375 (DYL72_10375) xerD 2154588..2155496 (+) 909 WP_013857679.1 site-specific tyrosine recombinase XerD -
  DYL72_RS10380 (DYL72_10380) - 2155510..2156283 (+) 774 WP_017045785.1 thioredoxin fold domain-containing protein -

Sequence

Protein


Download         Length: 562 a.a.        Molecular weight: 62291.43 Da        Isoelectric Point: 6.4458

>NTDB_id=332387 DYL72_RS10350 WP_029388146.1 2149948..2151636(-) (pilB) [Vibrio anguillarum strain J360]
MLTNLPAILRQAQILSPTQEHAVIEHIKASGANVPEALLALDFFHPHDLTEQLSTLFGLPDTDLTRYDYPQLCQQLGLRD
LITRYQALPIAHNGQTLTIAVSDPTHLQVEDDFRFATGLQVELVLADYKALQAAIRRLYGRTIQGSSSSGKEISQDELAN
LVKLSDDEIQSIEDLSQDDAPVSRFINQILLDAVRKGASDIHFEPYEALYRVRLRCDGILVETQRPSSNLSRRLAARLKI
LSKLDIAERRLPQDGRIKLRLNQDTAIDMRVSSLPTLWGEKIVLRLLDSSAANLDIDKLGYNDQQKQLYLAALKKPQGMI
LMTGPTGSGKTVSLYTGLRILNTAEVNISTAEDPVEINLSGINQVQVQPKIGFGFAQALRSFLRQDPDIVMVGEIRDLET
AEIAVKAAQTGHLVLSTLHTNSAAETVIRLSNMGIEAFNLASSLSLIIAQRLARRLCRHCKIAVVPSMQLQQHFQLLATE
TIYEANPHGCNECTGGYSGRVGIYEVMAFNPQLAEAIMQKASIHQIEQIAKANGMQTLQESGIEKLKNGVTSFKELQRVL
YF

Nucleotide


Download         Length: 1689 bp        

>NTDB_id=332387 DYL72_RS10350 WP_029388146.1 2149948..2151636(-) (pilB) [Vibrio anguillarum strain J360]
ATGCTAACCAACCTCCCTGCGATTCTGCGTCAGGCTCAGATATTGAGCCCGACGCAAGAACACGCAGTTATTGAACATAT
CAAAGCGTCTGGTGCGAATGTGCCAGAAGCTTTGCTCGCTTTGGATTTTTTCCATCCTCATGATCTGACTGAGCAGTTAA
GTACCCTTTTTGGCCTACCGGACACCGACTTAACCCGCTACGACTACCCACAGCTCTGCCAACAGCTTGGTTTGCGTGAT
CTCATCACCCGTTACCAAGCACTGCCGATTGCCCATAATGGCCAAACCCTGACCATCGCGGTTTCCGACCCGACCCATTT
ACAAGTGGAAGATGATTTTCGCTTCGCGACAGGCTTACAAGTTGAACTGGTTTTAGCCGACTACAAAGCGCTGCAAGCAG
CCATCCGGCGCTTGTATGGCCGAACGATTCAAGGATCCTCTTCTTCAGGGAAAGAGATCAGCCAAGATGAACTGGCGAAC
TTAGTTAAGCTATCTGATGATGAGATTCAATCGATTGAAGACCTCAGCCAAGATGACGCCCCAGTCAGCCGTTTTATTAA
CCAAATCCTGCTTGATGCGGTGCGAAAAGGCGCATCCGATATCCATTTCGAACCGTACGAAGCGCTTTACCGAGTACGAT
TGCGCTGTGATGGTATTTTGGTGGAAACGCAGCGTCCCTCAAGCAACTTAAGTCGCCGGCTTGCCGCGCGGCTTAAAATT
TTATCCAAACTCGATATTGCCGAGCGGCGCTTACCACAAGATGGACGAATTAAACTGCGGTTGAATCAAGACACCGCCAT
CGATATGCGCGTTTCCAGCCTCCCCACTTTATGGGGTGAAAAAATCGTGCTACGTCTACTCGATAGCAGCGCTGCTAACT
TAGATATCGACAAATTGGGTTATAACGACCAGCAAAAACAGCTCTACTTAGCTGCGCTGAAAAAACCACAAGGGATGATT
TTAATGACGGGCCCGACGGGCAGCGGAAAAACCGTCTCGCTCTATACGGGGCTACGCATTTTAAATACTGCTGAAGTGAA
CATTTCCACCGCTGAAGATCCGGTTGAAATTAACTTATCCGGTATCAACCAAGTTCAAGTGCAACCAAAAATTGGCTTTG
GCTTTGCGCAAGCATTACGCTCCTTCTTACGGCAAGATCCCGACATTGTGATGGTTGGCGAAATCCGCGATTTAGAAACT
GCCGAAATCGCCGTGAAAGCGGCACAAACCGGGCATTTAGTTCTTTCTACCTTGCATACTAACTCCGCCGCCGAAACGGT
AATTCGCTTGAGTAATATGGGAATTGAAGCGTTTAACTTAGCCTCTTCACTCAGTTTAATTATTGCCCAGCGTTTGGCTC
GTCGCCTTTGTCGGCACTGCAAAATTGCCGTCGTGCCCTCAATGCAGTTGCAGCAACATTTTCAGCTGCTAGCGACAGAA
ACCATTTATGAAGCGAACCCGCATGGTTGCAACGAATGCACAGGGGGCTATTCAGGTCGAGTCGGCATTTATGAAGTGAT
GGCGTTTAACCCACAGCTGGCCGAAGCGATTATGCAAAAAGCAAGCATTCATCAAATAGAACAGATCGCGAAAGCCAACG
GAATGCAGACACTACAAGAATCAGGCATTGAAAAGCTCAAAAACGGCGTCACCAGCTTCAAAGAGCTGCAACGCGTGCTC
TATTTCTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(38-141)

Type II/IV secretion system protein (T2SSE)

(188-455)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A289G7S6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Vibrio cholerae strain A1552

83.096

100

0.831
  pilB Vibrio campbellii strain DS40M4

74.377

100

0.744
  pilB Vibrio parahaemolyticus RIMD 2210633

72.954

100

0.73
  pilB Acinetobacter baumannii D1279779

52.115

92.527

0.482
  pilB Legionella pneumophila strain ERS1305867

51.758

91.103

0.472
  pilB Acinetobacter baylyi ADP1

50.38

93.594

0.472
  pilF Neisseria gonorrhoeae MS11

44.248

100

0.445
  pilF Thermus thermophilus HB27

37.589

100

0.377
  pilB/pilB1 Synechocystis sp. PCC 6803

35.763

100

0.375

Multiple sequence alignment