Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   NX08_RS16995 Genome accession   NZ_CP034657
Coordinates   3667322..3669055 (-) Length   577 a.a.
NCBI ID   WP_039440778.1    Uniprot ID   -
Organism   Xanthomonas vasicola strain NCPPB 902     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3662322..3674055
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NX08_RS16985 (NX08_016985) - 3663519..3665132 (+) 1614 WP_010375222.1 HAMP domain-containing sensor histidine kinase -
  NX08_RS16990 (NX08_016990) pilR 3665397..3666851 (+) 1455 WP_039440776.1 sigma-54 dependent transcriptional regulator Regulator
  NX08_RS16995 (NX08_016995) pilB 3667322..3669055 (-) 1734 WP_039440778.1 type IV-A pilus assembly ATPase PilB Machinery gene
  NX08_RS17000 (NX08_017000) - 3669117..3669551 (-) 435 WP_039440802.1 pilin -
  NX08_RS17005 (NX08_017005) pilE 3669626..3670066 (-) 441 WP_039440780.1 pilin Machinery gene
  NX08_RS17010 (NX08_017010) pilC 3670395..3671651 (+) 1257 WP_039440782.1 type II secretion system F family protein Machinery gene
  NX08_RS17015 (NX08_017015) - 3671658..3672521 (+) 864 WP_039440785.1 A24 family peptidase -
  NX08_RS17020 (NX08_017020) coaE 3672535..3673140 (+) 606 WP_010363800.1 dephospho-CoA kinase -

Sequence

Protein


Download         Length: 577 a.a.        Molecular weight: 62744.83 Da        Isoelectric Point: 5.4446

>NTDB_id=332169 NX08_RS16995 WP_039440778.1 3667322..3669055(-) (pilB) [Xanthomonas vasicola strain NCPPB 902]
MSVLSTANLVGITGIARRLVQDGALEESAARSAMDQAALAKVPLPQWFSEKKLVAASQLAAANAVEFGMPLLDVSAFDAN
QNAVKLVSEEFLQKHQVLPLFKRGNRLFVGVSNPTQTRALDDIKFHTNLVVEAILVDEDQIRRTLEQWQASNASLGSSLG
NDDDEMGDLDVSTGDEDMGAGGDSGVDARGDDTPVVKFVNKVLVDAIRRGASDIHFEPYEDDYRVRLRIDGLLKNVAKAP
VKLNQRIAARLKVMSQLDIAEKRVPQDGRIKLNLSKTKQIDFRVSTLPTLFGEKVVLRILDASAAKLGIEKLGYEADQQK
LFLDAIHKPYGMVLVTGPTGSGKTVSLYTALGILNDETRNISTAEDPVEIRLPGVNQVQQNVKRGMTFAAALRSFLRQDP
DIIMVGEIRDLETAEIAIKAAQTGHMVLSTLHTNDAPQTIARLMNMGIAPYNITSSVTLVIAQRLARRLCNNCKRKSTLP
DNALLAEGFTPEQIANMELYEAVGCDECTEGYKGRTGIYQVMPMTDEIGAIVLEGGNAMQIAEAAQAIGIRDLRQSALMK
AAHGVTSLAEINRVTKD

Nucleotide


Download         Length: 1734 bp        

>NTDB_id=332169 NX08_RS16995 WP_039440778.1 3667322..3669055(-) (pilB) [Xanthomonas vasicola strain NCPPB 902]
ATGAGTGTTTTATCGACTGCAAATCTCGTTGGGATCACCGGAATTGCTCGTAGGCTGGTGCAGGATGGTGCATTGGAGGA
AAGCGCGGCTCGAAGCGCCATGGATCAGGCTGCTCTTGCGAAAGTTCCATTGCCTCAGTGGTTCTCGGAGAAAAAGCTCG
TCGCTGCCTCACAGTTGGCGGCCGCCAACGCAGTCGAGTTCGGTATGCCGCTGCTCGATGTTTCTGCATTCGATGCCAAC
CAGAACGCGGTCAAGCTGGTGAGTGAGGAGTTTCTCCAGAAACACCAAGTGTTGCCGCTGTTCAAGCGCGGTAACCGGTT
GTTCGTGGGGGTGAGCAACCCGACCCAAACCCGGGCGCTGGATGACATTAAGTTCCATACGAACTTGGTGGTTGAGGCCA
TTCTTGTTGACGAGGATCAGATCCGTCGGACACTAGAACAGTGGCAGGCCAGCAACGCGTCTTTGGGTTCGTCGCTTGGT
AATGACGACGATGAGATGGGGGACTTGGACGTCTCCACCGGCGACGAAGACATGGGCGCCGGCGGGGATTCCGGGGTCGA
TGCCAGGGGCGACGACACGCCGGTGGTGAAGTTCGTCAACAAGGTGCTGGTGGATGCGATCAGGCGGGGAGCCTCGGACA
TCCATTTCGAGCCGTATGAAGACGACTACCGGGTGCGCTTGCGCATCGATGGGCTGTTGAAGAACGTGGCAAAGGCGCCG
GTGAAGCTGAATCAGCGCATCGCGGCGCGCCTGAAGGTGATGTCGCAGCTGGATATCGCCGAGAAGCGGGTGCCGCAGGA
CGGGCGCATCAAGCTCAACCTGTCTAAGACCAAGCAGATCGACTTTCGCGTGAGCACCTTGCCAACCCTGTTCGGCGAGA
AGGTGGTGCTGCGTATCCTGGACGCCAGCGCGGCCAAGCTCGGTATCGAGAAGCTGGGCTATGAGGCTGACCAGCAGAAG
CTGTTCCTGGATGCGATCCATAAGCCGTATGGCATGGTGCTGGTGACCGGGCCGACGGGCTCGGGTAAGACGGTGTCGCT
GTATACGGCGCTGGGCATCCTCAACGATGAGACCCGGAATATCTCAACGGCCGAAGATCCGGTCGAAATTCGCTTGCCTG
GCGTCAATCAGGTGCAGCAGAACGTCAAGCGCGGCATGACCTTTGCCGCTGCGCTGCGTTCGTTCCTGCGTCAGGACCCG
GACATCATTATGGTCGGCGAAATCCGTGACCTGGAGACGGCCGAGATTGCGATCAAGGCAGCGCAGACGGGCCACATGGT
GCTGTCGACGCTGCACACTAACGATGCGCCGCAGACCATTGCGCGACTGATGAATATGGGCATCGCGCCGTACAACATCA
CCTCGTCGGTAACGTTGGTGATCGCGCAGCGTCTGGCGCGGCGTTTGTGCAACAACTGCAAGCGCAAGTCGACGTTGCCG
GACAACGCATTGCTGGCGGAAGGTTTTACGCCCGAGCAAATCGCGAACATGGAGCTCTACGAGGCTGTGGGTTGCGACGA
GTGCACTGAAGGTTACAAGGGCCGTACCGGTATCTATCAGGTGATGCCGATGACCGACGAGATCGGCGCGATCGTGCTGG
AAGGCGGCAATGCGATGCAGATTGCCGAGGCCGCGCAGGCGATCGGTATCCGCGATTTGCGACAGTCGGCCTTGATGAAG
GCTGCGCACGGGGTGACCAGCCTGGCTGAGATCAATCGGGTGACGAAGGATTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(201-468)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(50-153)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

54.75

100

0.549
  pilB Acinetobacter baylyi ADP1

54.404

100

0.546
  pilB Legionella pneumophila strain ERS1305867

52.113

98.44

0.513
  pilF Neisseria gonorrhoeae MS11

48.944

98.44

0.482
  pilB Vibrio cholerae strain A1552

48.258

99.48

0.48
  pilB Vibrio campbellii strain DS40M4

46.221

98.614

0.456
  pilB Vibrio parahaemolyticus RIMD 2210633

45.471

97.574

0.444

Multiple sequence alignment