Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   EJN92_RS13630 Genome accession   NZ_CP034464
Coordinates   3112724..3114454 (-) Length   576 a.a.
NCBI ID   WP_126128334.1    Uniprot ID   A0A3Q9BRP3
Organism   Undibacterium parvum strain DSM 23061     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3107724..3119454
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EJN92_RS13605 (EJN92_13605) yacG 3108925..3109116 (-) 192 WP_126128329.1 DNA gyrase inhibitor YacG -
  EJN92_RS13610 (EJN92_13610) zapD 3109148..3109903 (-) 756 WP_126128330.1 cell division protein ZapD -
  EJN92_RS13615 (EJN92_13615) coaE 3109999..3110607 (-) 609 WP_126128331.1 dephospho-CoA kinase -
  EJN92_RS13620 (EJN92_13620) pilD 3110607..3111473 (-) 867 WP_126128332.1 A24 family peptidase Machinery gene
  EJN92_RS13625 (EJN92_13625) pilC 3111473..3112705 (-) 1233 WP_126128333.1 type II secretion system F family protein Machinery gene
  EJN92_RS13630 (EJN92_13630) pilB 3112724..3114454 (-) 1731 WP_126128334.1 type IV-A pilus assembly ATPase PilB Machinery gene
  EJN92_RS13635 (EJN92_13635) ispB 3115439..3116419 (-) 981 WP_227869540.1 octaprenyl diphosphate synthase -
  EJN92_RS13640 (EJN92_13640) - 3116832..3117839 (+) 1008 WP_126128335.1 glycosyl hydrolase family 18 protein -
  EJN92_RS13645 (EJN92_13645) rplU 3117979..3118290 (+) 312 WP_126128336.1 50S ribosomal protein L21 -
  EJN92_RS13650 (EJN92_13650) rpmA 3118338..3118613 (+) 276 WP_126128337.1 50S ribosomal protein L27 -

Sequence

Protein


Download         Length: 576 a.a.        Molecular weight: 62820.64 Da        Isoelectric Point: 5.8134

>NTDB_id=331495 EJN92_RS13630 WP_126128334.1 3112724..3114454(-) (pilB) [Undibacterium parvum strain DSM 23061]
MSAVSPNSAAQASTSSGLARALIQANVLSLAQIDPIHKKSLVEKSHFVDGLLQSGLINARDLATFCSETFGYPLVDLAVF
NESMLPEKIVNVKLMQAQRMVALAKKGNKISLAISDPTNTNALDQIKFQTGLGIELVIVQHDILLKLLEKLSKTSEQSIA
DLAGDDYNIEFNEEDMSSGVPDTQVSEVDDAPVVKFLQKMLIDAINMGASDLHFEPFEKFYRIRFRVDGELREIVQPPIA
IKDKLVSRIKVISKLDISEKRVPQDGRMKLTLSPTKAIDFRVSVLPTLFGEKIVMRILDGSQAQMGIDALGYDPDQKAIL
MDAIQRPYGMVLVTGPTGSGKTVSLYTCLNVINKPGINISTAEDPAEINLPGVNQVNINDRAGLTFPVALKAFLRQDPDI
IMVGEIRDLETADIAIKAAQTGHMVFSTLHTNDAPSTLTRLMNMGVAPFNIASSVILITAQRLTRRLCTCKKPVDILDEV
LLQAGFKENELDGSWKPYGPVGCERCSGSGYKGRVGIYQIMPITEAIERIILANGSSLEIKKQSEKEGVRGLRQSGLQKV
KQGATSLEEVLGCTND

Nucleotide


Download         Length: 1731 bp        

>NTDB_id=331495 EJN92_RS13630 WP_126128334.1 3112724..3114454(-) (pilB) [Undibacterium parvum strain DSM 23061]
ATGTCTGCTGTTTCACCAAATTCTGCTGCCCAAGCTTCTACCTCATCGGGCCTCGCTAGGGCGCTGATACAGGCCAATGT
ATTATCATTGGCGCAAATCGATCCTATCCACAAAAAATCATTGGTCGAAAAAAGTCACTTTGTCGACGGTTTGCTCCAAA
GTGGCTTAATCAATGCTCGCGATCTGGCGACGTTTTGCTCGGAAACCTTTGGTTACCCTTTGGTGGATTTGGCGGTGTTT
AATGAAAGCATGCTGCCTGAAAAAATTGTCAACGTAAAACTCATGCAAGCACAACGCATGGTGGCCTTGGCGAAAAAGGG
CAATAAAATTTCACTGGCGATCTCCGACCCAACCAATACCAATGCCTTAGATCAAATCAAATTCCAGACTGGACTGGGAA
TAGAATTGGTGATCGTTCAGCACGATATTCTCCTCAAATTACTAGAAAAGCTGAGCAAAACCTCTGAACAAAGCATTGCA
GATTTAGCCGGTGATGACTACAACATCGAGTTTAATGAAGAGGACATGAGCTCGGGTGTCCCGGATACACAAGTAAGCGA
AGTCGATGACGCACCGGTAGTTAAATTTTTACAGAAGATGTTAATTGACGCGATTAATATGGGCGCCTCAGATTTACATT
TTGAACCTTTCGAAAAATTTTACCGGATACGTTTTCGGGTTGACGGTGAGCTGAGGGAAATTGTCCAGCCGCCCATCGCG
ATTAAGGACAAACTAGTTTCGCGTATCAAAGTTATTTCTAAATTGGATATTTCGGAAAAGCGTGTACCACAAGATGGCCG
CATGAAACTGACCTTGTCGCCAACCAAAGCTATCGACTTCCGGGTCAGCGTACTGCCGACCTTGTTCGGCGAAAAAATAG
TTATGCGTATCCTCGATGGATCGCAAGCCCAAATGGGTATTGATGCTCTTGGCTATGATCCGGATCAAAAAGCCATACTA
ATGGATGCGATTCAAAGACCTTACGGCATGGTCTTGGTCACAGGACCGACTGGCTCAGGAAAAACGGTCTCTCTATATAC
GTGCTTAAACGTTATAAATAAGCCTGGTATAAATATCTCAACGGCAGAAGATCCTGCCGAGATTAACCTACCAGGTGTGA
ATCAAGTCAATATCAATGATAGAGCAGGTCTGACCTTTCCAGTCGCACTCAAGGCGTTTTTACGCCAGGATCCTGACATT
ATCATGGTCGGTGAGATTCGCGATCTGGAAACGGCTGACATAGCAATTAAAGCCGCGCAAACAGGTCACATGGTATTTTC
CACCTTGCATACCAATGATGCGCCGTCGACCTTGACCCGTCTGATGAATATGGGGGTCGCACCTTTTAATATTGCCTCCT
CAGTCATTTTGATCACGGCACAGAGGTTGACACGTCGACTTTGCACCTGCAAAAAGCCGGTTGATATTTTAGATGAGGTT
TTGTTGCAAGCCGGCTTCAAGGAAAATGAACTCGATGGCAGTTGGAAGCCCTATGGCCCAGTTGGTTGCGAGCGCTGTAG
CGGTAGCGGCTACAAAGGCAGGGTTGGCATTTATCAGATCATGCCAATTACCGAAGCAATAGAACGCATCATTCTGGCCA
ATGGCTCTTCACTTGAGATCAAAAAGCAATCGGAAAAAGAGGGCGTCAGGGGACTAAGACAATCCGGCCTACAAAAAGTA
AAGCAAGGTGCTACTAGCTTAGAAGAAGTACTAGGTTGCACCAACGACTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(199-466)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(51-155)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A3Q9BRP3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

53.286

97.743

0.521
  pilF Neisseria gonorrhoeae MS11

52.281

98.958

0.517
  pilB Acinetobacter baylyi ADP1

51.687

97.743

0.505
  pilB Legionella pneumophila strain ERS1305867

48.94

98.264

0.481
  pilB Vibrio cholerae strain A1552

45.053

98.264

0.443
  pilB Vibrio parahaemolyticus RIMD 2210633

45.053

98.264

0.443
  pilB Vibrio campbellii strain DS40M4

43.794

97.917

0.429
  pilF Thermus thermophilus HB27

39.851

93.229

0.372
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.757

94.965

0.368

Multiple sequence alignment