Detailed information    

insolico Bioinformatically predicted

Overview


Name   ssbA   Type   Machinery gene
Locus tag   EJN89_RS01650 Genome accession   NZ_CP034441
Coordinates   314212..314682 (-) Length   156 a.a.
NCBI ID   WP_016047540.1    Uniprot ID   -
Organism   Staphylococcus aureus strain PMB 81-4     
Function   ssDNA binding (predicted from homology)   
DNA processing

Related MGE


Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Prophage 281153..324844 314212..314682 within 0


Gene organization within MGE regions


Location: 281153..324844
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EJN89_RS01420 - 281153..281710 (-) 558 WP_000528632.1 PBECR4 domain-containing protein -
  EJN89_RS01425 - 282435..283889 (-) 1455 WP_000909217.1 N-acetylmuramoyl-L-alanine amidase -
  EJN89_RS01430 - 283900..284202 (-) 303 WP_125998305.1 phage holin -
  EJN89_RS01435 - 284258..284653 (-) 396 WP_000398878.1 hypothetical protein -
  EJN89_RS01440 - 284659..285831 (-) 1173 WP_001837327.1 BppU family phage baseplate upper protein -
  EJN89_RS01445 - 285844..287742 (-) 1899 WP_001837328.1 glucosaminidase domain-containing protein -
  EJN89_RS01450 - 287879..288178 (-) 300 WP_000466784.1 DUF2951 domain-containing protein -
  EJN89_RS01455 - 288219..288392 (-) 174 WP_015990323.1 XkdX family protein -
  EJN89_RS01460 - 288383..288778 (-) 396 WP_125998307.1 DUF2977 domain-containing protein -
  EJN89_RS01465 - 288778..290601 (-) 1824 WP_016047530.1 BppU family phage baseplate upper protein -
  EJN89_RS01470 - 290601..292511 (-) 1911 WP_000369018.1 hypothetical protein -
  EJN89_RS01475 - 292526..294427 (-) 1902 WP_001657243.1 SGNH/GDSL hydrolase family protein -
  EJN89_RS01480 - 294436..295383 (-) 948 WP_001565386.1 phage tail family protein -
  EJN89_RS01485 - 295396..298863 (-) 3468 WP_103150437.1 hypothetical protein -
  EJN89_RS01490 - 298880..299224 (-) 345 WP_001837332.1 hypothetical protein -
  EJN89_RS01495 - 299254..299619 (-) 366 WP_001100161.1 tail assembly chaperone -
  EJN89_RS01500 - 299681..300262 (-) 582 WP_001837333.1 phage major tail protein, TP901-1 family -
  EJN89_RS01505 - 300281..300664 (-) 384 WP_001837334.1 hypothetical protein -
  EJN89_RS01510 - 300676..301023 (-) 348 WP_001017811.1 HK97-gp10 family putative phage morphogenesis protein -
  EJN89_RS01515 - 301023..301325 (-) 303 WP_001268309.1 hypothetical protein -
  EJN89_RS01520 - 301322..301654 (-) 333 WP_000208960.1 phage head-tail connector protein -
  EJN89_RS01525 - 301663..301950 (-) 288 WP_001837335.1 hypothetical protein -
  EJN89_RS01530 - 301972..302946 (-) 975 WP_001837336.1 phage major capsid protein -
  EJN89_RS01535 - 302960..303574 (-) 615 WP_000354309.1 DUF4355 domain-containing protein -
  EJN89_RS01540 - 303709..303879 (-) 171 WP_000072207.1 hypothetical protein -
  EJN89_RS01545 - 303952..304947 (-) 996 WP_001837337.1 minor capsid protein -
  EJN89_RS01550 - 304954..306489 (-) 1536 WP_001837338.1 phage portal protein -
  EJN89_RS01555 - 306500..307777 (-) 1278 WP_016047536.1 PBSX family phage terminase large subunit -
  EJN89_RS01560 - 307764..308204 (-) 441 WP_001003271.1 terminase small subunit -
  EJN89_RS01565 - 308391..308810 (-) 420 WP_000058632.1 transcriptional regulator -
  EJN89_RS01570 - 308825..308971 (-) 147 WP_000989998.1 hypothetical protein -
  EJN89_RS01575 - 308972..309337 (-) 366 WP_000455357.1 hypothetical protein -
  EJN89_RS01580 - 309338..309511 (-) 174 WP_000595256.1 transcriptional activator RinB -
  EJN89_RS01585 - 309508..309714 (-) 207 WP_000195810.1 DUF1381 domain-containing protein -
  EJN89_RS01590 - 309751..310281 (-) 531 WP_000185706.1 dUTPase -
  EJN89_RS01595 - 310274..310480 (-) 207 WP_000097971.1 Lar family restriction alleviation protein -
  EJN89_RS01600 - 310480..310722 (-) 243 WP_001065066.1 DUF1024 family protein -
  EJN89_RS01605 - 310715..310999 (-) 285 WP_125998309.1 hypothetical protein -
  EJN89_RS01610 - 310996..311445 (-) 450 WP_016047537.1 YopX family protein -
  EJN89_RS15085 - 311442..311636 (-) 195 WP_000983954.1 hypothetical protein -
  EJN89_RS01620 - 311633..312019 (-) 387 WP_000693615.1 hypothetical protein -
  EJN89_RS01625 - 312033..312275 (-) 243 WP_001837466.1 SAV1978 family virulence-associated passenger protein -
  EJN89_RS01630 - 312279..312647 (-) 369 WP_000101275.1 SA1788 family PVL leukocidin-associated protein -
  EJN89_RS01635 - 312660..313064 (-) 405 WP_029052043.1 RusA family crossover junction endodeoxyribonuclease -
  EJN89_RS01640 - 313073..313291 (-) 219 WP_000338528.1 hypothetical protein -
  EJN89_RS01645 - 313298..314182 (-) 885 WP_016047539.1 DnaD domain protein -
  EJN89_RS01650 ssbA 314212..314682 (-) 471 WP_016047540.1 single-stranded DNA-binding protein Machinery gene
  EJN89_RS01655 - 314683..315300 (-) 618 WP_072528355.1 MBL fold metallo-hydrolase -
  EJN89_RS01660 - 315381..316301 (-) 921 WP_000138475.1 recombinase RecT -
  EJN89_RS01665 - 316303..318246 (-) 1944 WP_000700571.1 AAA family ATPase -
  EJN89_RS01670 - 318255..318518 (-) 264 WP_001205732.1 hypothetical protein -
  EJN89_RS01675 - 318527..318787 (-) 261 WP_001837398.1 DUF1108 family protein -
  EJN89_RS01680 - 318851..319165 (-) 315 WP_001280581.1 helix-turn-helix transcriptional regulator -
  EJN89_RS01685 - 319170..319340 (-) 171 WP_001837399.1 DUF1270 domain-containing protein -
  EJN89_RS15185 - 319333..319461 (-) 129 WP_001559112.1 hypothetical protein -
  EJN89_RS01690 - 319520..319765 (+) 246 WP_000122246.1 hypothetical protein -
  EJN89_RS01695 - 319733..319936 (-) 204 Protein_323 hypothetical protein -
  EJN89_RS01700 - 319989..320183 (-) 195 WP_000390105.1 hypothetical protein -
  EJN89_RS01705 - 320200..320988 (-) 789 WP_001148580.1 phage antirepressor KilAC domain-containing protein -
  EJN89_RS01710 - 321004..321258 (-) 255 Protein_326 helix-turn-helix transcriptional regulator -
  EJN89_RS01715 - 321449..322087 (+) 639 WP_000874711.1 XRE family transcriptional regulator -
  EJN89_RS01720 - 322144..322329 (+) 186 WP_000446800.1 hypothetical protein -
  EJN89_RS01725 - 322382..322882 (+) 501 WP_001837400.1 hypothetical protein -
  EJN89_RS01730 - 322886..323401 (+) 516 WP_000826264.1 hypothetical protein -
  EJN89_RS01735 - 323459..324844 (+) 1386 WP_000861313.1 recombinase family protein -

Sequence


Protein


Download         Length: 156 a.a.        Molecular weight: 17662.58 Da        Isoelectric Point: 4.9628

>NTDB_id=331285 EJN89_RS01650 WP_016047540.1 314212..314682(-) (ssbA) [Staphylococcus aureus strain PMB 81-4]
MLNRVVLVGRLTKDPEYRTTPSGVSVASFTLAVNRTFTNAQGEREADFINIIVFKKQAENVNKYLSKGSLTGVDGRLQTR
NYENKEGQRVYVTEVIADSIQFLEPKNSNDTQQDLYQQQVQQTRGQSQYSNNKPVKDNPFANANVPIEIDDDDLPF

Nucleotide


Download         Length: 471 bp        

>NTDB_id=331285 EJN89_RS01650 WP_016047540.1 314212..314682(-) (ssbA) [Staphylococcus aureus strain PMB 81-4]
ATGCTAAATAGAGTTGTATTAGTAGGTCGTTTAACGAAAGATCCGGAATACAGAACCACTCCTTCAGGTGTGAGTGTAGC
ATCATTCACATTAGCAGTTAACCGCACATTTACGAATGCACAAGGAGAGCGCGAGGCAGACTTTATTAATATCATCGTAT
TTAAAAAACAAGCAGAGAACGTTAATAAATACCTATCTAAAGGATCGTTGACGGGCGTAGATGGTAGGTTACAAACGCGG
AATTATGAAAATAAGGAAGGTCAACGTGTATATGTTACGGAAGTTATTGCTGATAGTATTCAATTTTTAGAACCGAAAAA
CTCAAATGACACTCAACAAGATTTATACCAACAACAAGTACAACAAACACGTGGACAATCGCAATATTCAAATAACAAAC
CAGTAAAAGATAATCCGTTTGCGAATGCAAATGTTCCGATTGAAATAGATGACGATGATTTACCATTCTAA


Secondary structure


Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure


Source ID Structure

Transmembrane helices


Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.



Visualization of predicted probability:


Similar proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ssbA Bacillus subtilis subsp. subtilis str. 168

57.627

100

0.654

  ssb Latilactobacillus sakei subsp. sakei 23K

51.176

100

0.558

  ssbB Bacillus subtilis subsp. subtilis str. 168

60.377

67.949

0.41

  ssb Glaesserella parasuis strain SC1401

33.333

100

0.378

  ssb Vibrio cholerae strain A1552

31.492

100

0.365

  ssb Neisseria meningitidis MC58

32.948

100

0.365

  ssb Neisseria gonorrhoeae MS11

32.948

100

0.365


Multiple sequence alignment