Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   EJO50_RS11230 Genome accession   NZ_CP034433
Coordinates   2599733..2601442 (+) Length   569 a.a.
NCBI ID   WP_125974223.1    Uniprot ID   A0A3S8ZU86
Organism   Iodobacter ciconiae strain H11R3     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2594733..2606442
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EJO50_RS11205 (EJO50_11205) rimM 2595003..2595536 (-) 534 WP_308418400.1 ribosome maturation factor RimM -
  EJO50_RS11210 (EJO50_11210) rpsP 2595545..2595796 (-) 252 WP_125974215.1 30S ribosomal protein S16 -
  EJO50_RS11215 (EJO50_11215) ffh 2596049..2597401 (-) 1353 WP_125974217.1 signal recognition particle protein -
  EJO50_RS11220 (EJO50_11220) - 2597457..2598305 (+) 849 WP_125974219.1 inner membrane protein YpjD -
  EJO50_RS11225 (EJO50_11225) - 2598437..2599621 (+) 1185 WP_233702057.1 HlyC/CorC family transporter -
  EJO50_RS11230 (EJO50_11230) pilB 2599733..2601442 (+) 1710 WP_125974223.1 type IV-A pilus assembly ATPase PilB Machinery gene
  EJO50_RS11235 (EJO50_11235) pilC 2601461..2602684 (+) 1224 WP_125974225.1 type II secretion system F family protein Machinery gene
  EJO50_RS11240 (EJO50_11240) pilD 2602684..2603547 (+) 864 WP_125974227.1 A24 family peptidase Machinery gene
  EJO50_RS11245 (EJO50_11245) coaE 2603547..2604173 (+) 627 WP_233702058.1 dephospho-CoA kinase -
  EJO50_RS11250 (EJO50_11250) zapD 2604234..2604992 (+) 759 WP_125974229.1 cell division protein ZapD -
  EJO50_RS11255 (EJO50_11255) - 2604989..2605180 (+) 192 WP_125974231.1 DNA gyrase inhibitor YacG -
  EJO50_RS17780 (EJO50_11260) - 2605387..2605563 (+) 177 WP_373280514.1 GNAT family N-acyltransferase -
  EJO50_RS17785 - 2605505..2605630 (+) 126 WP_373280532.1 GNAT family N-acetyltransferase -

Sequence

Protein


Download         Length: 569 a.a.        Molecular weight: 62275.85 Da        Isoelectric Point: 5.4099

>NTDB_id=331218 EJO50_RS11230 WP_125974223.1 2599733..2601442(+) (pilB) [Iodobacter ciconiae strain H11R3]
MSAPNSPVSGLARLLVQHGRLIEADAETLQSTANSSKTPFIEQLIRSHKMTARGIAEFSSQAFGYPLLDLDHIDPSYIPQ
GVLDNKVMQIQRLVPLFKRGTKLFIGLSDITNLQALEEVRFQTGLQVQPLVVEDGKLVILLNKIVESTGANLSNMALDDV
NLEMDGGEEDTSQNDAAVLEVDDAPIVKYLQKILLDAINAGVSDIHFEPYEKFFRIRYRLDGVLREVAQPPLAIKDKLAS
RIKVISKLDISEKRVPQDGRMKLVLSKSRAIDFRVSTMPTMHGEKICMRILDPSSATLGIDALGYDPDQKEILLEAIQRP
YGMILVTGPTGSGKTVSLYTCLNILNQPGINISTAEDPCEINLPGVNQVNVNEKSGLTFAVALKAFLRQDPDIIMVGEIR
DLGTADISIKAAQTGHMVFSTLHTNDAPTTLTRMLNMGIAPFNIASSVILITAQRLARKLCSCKTPIDIPHQALLDAGFE
EEELDGTWSPYKPVGCDICKGSGYKGRVGIYQVMPITDAMNRIIMTNGNAIDIADQAKRDGVRDLRGSGLRKVKQGLTSL
EEVMAVTNE

Nucleotide


Download         Length: 1710 bp        

>NTDB_id=331218 EJO50_RS11230 WP_125974223.1 2599733..2601442(+) (pilB) [Iodobacter ciconiae strain H11R3]
ATGTCAGCACCTAACTCACCTGTATCTGGTCTCGCCCGCCTGCTAGTTCAACACGGCAGATTAATTGAAGCAGATGCTGA
AACGCTGCAATCCACAGCGAACAGCAGCAAAACACCATTTATTGAGCAACTCATACGCAGTCATAAAATGACGGCGCGGG
GTATTGCTGAATTCTCATCCCAGGCGTTTGGCTATCCTCTTCTTGATCTGGACCATATTGATCCGAGCTATATCCCTCAA
GGCGTGCTGGACAATAAAGTAATGCAGATCCAACGCCTCGTGCCACTATTCAAACGTGGTACCAAGCTATTTATCGGGCT
ATCTGATATTACCAACCTGCAAGCGCTCGAAGAAGTGCGCTTTCAAACCGGACTACAAGTTCAGCCCCTGGTAGTAGAAG
ACGGTAAGCTTGTTATTCTGCTCAACAAAATTGTCGAATCTACAGGGGCCAATCTAAGTAATATGGCTCTGGATGATGTC
AATTTAGAGATGGATGGGGGCGAGGAAGATACCAGCCAAAACGATGCCGCAGTTCTGGAAGTAGATGATGCACCCATCGT
TAAGTATCTGCAAAAAATCCTGCTTGATGCCATCAATGCAGGTGTTTCCGATATTCACTTTGAGCCCTATGAAAAGTTCT
TTCGCATCCGTTACCGCCTTGACGGCGTATTGCGTGAAGTAGCGCAACCTCCCTTGGCGATTAAAGATAAACTTGCCTCA
CGTATCAAAGTTATCTCCAAGCTCGACATCTCTGAAAAACGTGTACCGCAAGATGGCCGAATGAAACTTGTGCTTTCAAA
AAGCCGAGCCATCGACTTTCGCGTGTCTACCATGCCAACAATGCATGGAGAAAAGATTTGTATGCGGATTCTGGATCCCT
CTTCAGCCACCTTGGGTATTGATGCCCTAGGGTATGATCCGGATCAAAAAGAAATCTTGCTGGAAGCCATTCAGCGCCCT
TACGGTATGATATTAGTAACTGGCCCGACAGGTTCGGGGAAGACAGTTTCGCTTTATACCTGCCTGAATATTCTAAATCA
GCCGGGTATTAACATCTCCACTGCTGAAGACCCCTGTGAAATTAACCTGCCTGGCGTAAACCAGGTCAATGTCAACGAAA
AGTCTGGACTGACATTTGCTGTTGCACTAAAGGCCTTCTTGCGTCAGGATCCTGACATCATCATGGTTGGGGAAATCCGT
GATTTAGGCACAGCAGATATATCCATCAAGGCTGCCCAAACAGGGCATATGGTTTTTTCTACCCTGCATACCAACGATGC
ACCCACTACGCTGACACGGATGCTCAATATGGGAATTGCCCCATTTAATATTGCATCTTCGGTTATTTTAATTACAGCGC
AGCGTTTGGCCCGAAAGCTATGCAGCTGTAAAACACCAATAGATATTCCTCATCAGGCACTACTCGATGCAGGTTTTGAA
GAAGAAGAGCTGGACGGCACCTGGAGCCCTTATAAACCCGTTGGCTGTGATATTTGCAAGGGCTCAGGCTACAAAGGCCG
CGTAGGGATTTATCAGGTAATGCCAATTACTGATGCCATGAACCGCATCATTATGACTAATGGAAATGCAATTGATATTG
CTGACCAGGCAAAACGTGATGGCGTGCGTGATTTACGAGGCTCTGGCTTACGTAAAGTAAAACAAGGCCTGACCTCACTA
GAAGAAGTTATGGCGGTAACCAATGAATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(192-459)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(50-146)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A3S8ZU86

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

54.878

100

0.554
  pilB Acinetobacter baumannii D1279779

55.044

99.297

0.547
  pilF Neisseria gonorrhoeae MS11

53.286

98.946

0.527
  pilB Legionella pneumophila strain ERS1305867

52.557

99.649

0.524
  pilB Vibrio cholerae strain A1552

46.691

98.243

0.459
  pilB Vibrio parahaemolyticus RIMD 2210633

45.263

100

0.453
  pilB Vibrio campbellii strain DS40M4

44.681

99.121

0.443
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.662

93.497

0.371
  pilF Thermus thermophilus HB27

36.46

99.297

0.362

Multiple sequence alignment