Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   EG364_RS20890 Genome accession   NZ_CP033862
Coordinates   29029..29889 (-) Length   286 a.a.
NCBI ID   WP_001152285.1    Uniprot ID   -
Organism   Acinetobacter sp. FDAARGOS_560     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 24029..34889
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EG364_RS00130 (EG364_00130) - 24920..26230 (+) 1311 WP_000753294.1 tetratricopeptide repeat protein -
  EG364_RS00135 (EG364_00135) - 26307..26633 (+) 327 WP_000099685.1 pyrimidine/purine nucleoside phosphorylase -
  EG364_RS00140 (EG364_00140) rlmB 26735..27484 (+) 750 WP_001118149.1 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB -
  EG364_RS00145 (EG364_00145) - 27520..28434 (+) 915 WP_000075011.1 DMT family transporter -
  EG364_RS00150 (EG364_00150) coaE 28431..29027 (-) 597 WP_000874125.1 dephospho-CoA kinase -
  EG364_RS20890 (EG364_00155) pilD 29029..29889 (-) 861 WP_001152285.1 prepilin peptidase Machinery gene
  EG364_RS00160 (EG364_00160) pilC 29889..31115 (-) 1227 WP_000279216.1 type II secretion system F family protein Machinery gene
  EG364_RS00165 (EG364_00165) pilB 31145..32857 (-) 1713 WP_001274990.1 type IV-A pilus assembly ATPase PilB Machinery gene
  EG364_RS00170 (EG364_00170) tpiA 33149..33943 (+) 795 WP_000016939.1 triose-phosphate isomerase -
  EG364_RS00175 (EG364_00175) secG 33956..34285 (+) 330 WP_000555572.1 preprotein translocase subunit SecG -

Sequence

Protein


Download         Length: 286 a.a.        Molecular weight: 32153.54 Da        Isoelectric Point: 7.1042

>NTDB_id=326540 EG364_RS20890 WP_001152285.1 29029..29889(-) (pilD) [Acinetobacter sp. FDAARGOS_560]
MQDIIAYFIQNLTALYIAVALVSLCIGSFLNVVIYRTPRMMEQDWQQECQMLLNPEQPIIDHERLTLNKPASSCPACQQP
IRWYQNIPVISWLVLRGKCGHCQHPISIRYPAIELLTMLCSLVVVMVFGPTIQMLFGLILTWVLIALTFIDFDTQLLPDR
FTLPLAALGLGINTFNIYTSPNSAIWGYLIGFLCLWIVYYLFKVITGKEGMGYGDFKLLAALGAWMGPLMLPLIVLLSSL
IGAIIGIILLKLRNDNQPFAFGPYIAIAGWVAFLWGDQIMKIYLGG

Nucleotide


Download         Length: 861 bp        

>NTDB_id=326540 EG364_RS20890 WP_001152285.1 29029..29889(-) (pilD) [Acinetobacter sp. FDAARGOS_560]
ATGCAAGACATCATTGCGTATTTTATTCAAAACTTAACTGCACTTTATATTGCTGTTGCACTCGTGAGCCTATGTATCGG
TAGCTTTCTTAATGTAGTAATTTACCGCACGCCAAGAATGATGGAGCAAGATTGGCAGCAAGAATGCCAAATGTTACTCA
ATCCTGAGCAACCAATTATTGATCATGAGAGGTTAACTTTAAATAAGCCTGCTTCATCGTGCCCTGCATGTCAGCAACCG
ATCCGTTGGTATCAAAATATTCCTGTTATAAGCTGGCTTGTGTTAAGAGGAAAATGTGGCCATTGCCAACACCCGATCAG
TATTCGCTATCCAGCCATCGAACTACTCACCATGCTATGTTCATTAGTAGTAGTCATGGTATTTGGCCCAACCATACAAA
TGCTTTTTGGACTCATCCTCACATGGGTACTGATAGCCCTTACCTTTATTGATTTCGATACACAATTATTGCCTGATCGC
TTTACCCTACCTTTAGCTGCGCTCGGCTTAGGTATTAATACCTTTAATATTTATACCTCACCCAACTCAGCCATTTGGGG
TTATCTCATTGGTTTCCTATGTCTTTGGATTGTCTATTACTTATTTAAAGTGATCACTGGCAAAGAAGGTATGGGCTACG
GCGACTTTAAATTGCTTGCAGCATTAGGAGCATGGATGGGGCCATTGATGCTGCCGTTAATTGTGTTATTGTCATCGTTA
ATTGGCGCAATCATTGGCATCATTTTATTAAAATTAAGAAATGACAATCAGCCTTTTGCTTTTGGGCCATATATTGCCAT
TGCTGGTTGGGTTGCCTTTTTATGGGGTGATCAGATTATGAAAATTTATTTGGGAGGTTAA

Domains

Predicted by InterproScan.

Type IV leader peptidase family (Peptidase_A24)

(138-247)

Bacterial Peptidase A24 N-terminal domain (A24_N_bact)

(22-128)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Acinetobacter baumannii D1279779

98.951

100

0.99
  pilD Acinetobacter nosocomialis M2

97.902

100

0.979
  pilD Vibrio cholerae strain A1552

52.941

95.105

0.503
  pilD Vibrio campbellii strain DS40M4

52.03

94.755

0.493
  pilD Neisseria gonorrhoeae MS11

48.485

92.308

0.448

Multiple sequence alignment