Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   EGY09_RS15740 Genome accession   NZ_CP033829
Coordinates   3300832..3302565 (-) Length   577 a.a.
NCBI ID   WP_123958964.1    Uniprot ID   -
Organism   Stenotrophomonas maltophilia strain FDAARGOS_507     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3295832..3307565
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EGY09_RS15725 (EGY09_15700) sucC 3296132..3297301 (-) 1170 WP_004154430.1 ADP-forming succinate--CoA ligase subunit beta -
  EGY09_RS15730 (EGY09_15705) - 3297659..3299272 (+) 1614 WP_005414304.1 ATP-binding protein -
  EGY09_RS15735 (EGY09_15710) pilR 3299333..3300706 (+) 1374 WP_005410795.1 sigma-54 dependent transcriptional regulator Regulator
  EGY09_RS15740 (EGY09_15715) pilB 3300832..3302565 (-) 1734 WP_123958964.1 type IV-A pilus assembly ATPase PilB Machinery gene
  EGY09_RS15745 (EGY09_15720) pilA/pilAI 3302788..3303195 (-) 408 WP_114619185.1 pilin Machinery gene
  EGY09_RS15750 (EGY09_15725) - 3303350..3303790 (-) 441 WP_005410798.1 pilin -
  EGY09_RS15755 (EGY09_15730) pilC 3304143..3305402 (+) 1260 WP_099605875.1 type II secretion system F family protein Machinery gene
  EGY09_RS15760 (EGY09_15735) - 3305410..3306273 (+) 864 WP_123958966.1 A24 family peptidase -
  EGY09_RS15765 (EGY09_15740) coaE 3306285..3306896 (+) 612 WP_123958968.1 dephospho-CoA kinase -
  EGY09_RS15770 (EGY09_15745) - 3307156..3307542 (-) 387 WP_005410802.1 hypothetical protein -

Sequence

Protein


Download         Length: 577 a.a.        Molecular weight: 62317.25 Da        Isoelectric Point: 5.8722

>NTDB_id=326197 EGY09_RS15740 WP_123958964.1 3300832..3302565(-) (pilB) [Stenotrophomonas maltophilia strain FDAARGOS_507]
MNAVTTANLVGITGLARRLVQDGALDEATARDAMAKAAAARQPLPTWFAQNKLVNASQLAAANAVEFGMPLFDVSTFDAS
QNAMSLVSEELLRKHNVLPLFKRGGKLFVGTSNPTHSLDEIKFHTNLVVEPILVDEDQIRRTLEQWHASHDTLGDALGGD
DDGMGNLDVAAGDEDLGATGGESGIDAKGDDTPVVKFVNKVLVDAIRKGASDIHFEPYEDDYRVRLRIDGLLKMVARAPV
KLNQRIAARLKVMAQLDIAEKRVPQDGRIKLNLSKTKQIDFRVSTLPTLFGEKVVLRILDGSAAKLGIDKLGYEPDQQKL
FLDAIHKPYGMVLVTGPTGSGKTVSLYTALGILNDETRNISTAEDPVEIRLPGVNQVQQNNKRGMTFAAALRSFLRQDPD
IIMVGEIRDLETAEIAIKAAQTGHMVLSTLHTNDAPQTIARLMNMGIAPYNITSSVTLVIAQRLARRLCGNCKRPSSLPD
HALLAEGFTQAQLDAGIELYEAVGCDECTEGYKGRTGVYQVMPMTDEIATIVLAGGNALQIAEAAQQIGVNDLRQSALKK
AAAGITSLAEINRVTKD

Nucleotide


Download         Length: 1734 bp        

>NTDB_id=326197 EGY09_RS15740 WP_123958964.1 3300832..3302565(-) (pilB) [Stenotrophomonas maltophilia strain FDAARGOS_507]
ATGAACGCTGTCACCACCGCCAATCTTGTCGGCATCACCGGCCTGGCCCGTCGCCTGGTGCAGGACGGGGCCCTGGACGA
GGCCACCGCCCGCGACGCCATGGCCAAGGCCGCCGCCGCGCGCCAGCCACTGCCGACCTGGTTCGCGCAGAACAAGCTGG
TCAATGCCTCTCAGCTGGCCGCCGCCAACGCGGTCGAGTTCGGCATGCCCCTGTTCGACGTGTCCACCTTCGACGCCAGC
CAGAACGCGATGAGCCTGGTCAGCGAGGAGCTGCTGCGCAAGCACAACGTGCTGCCGCTGTTCAAGCGCGGTGGCAAGCT
GTTCGTCGGCACCAGCAACCCGACCCACTCGCTGGACGAGATCAAGTTCCACACCAACCTGGTGGTCGAGCCGATCCTGG
TCGACGAGGACCAGATCCGGCGCACGCTGGAACAGTGGCACGCCAGCCATGACACCCTCGGCGATGCCCTGGGTGGCGAT
GACGATGGCATGGGCAACCTGGACGTGGCGGCCGGCGACGAGGATCTCGGCGCCACCGGCGGCGAGTCCGGCATCGATGC
CAAGGGTGACGACACCCCGGTGGTGAAGTTCGTCAACAAGGTGCTGGTCGATGCGATCCGCAAGGGCGCATCGGACATCC
ACTTCGAGCCCTACGAAGACGATTACCGCGTGCGCCTGCGCATCGACGGCCTGCTGAAGATGGTCGCGCGCGCACCGGTG
AAGCTGAACCAGCGCATCGCCGCGCGCCTGAAGGTGATGGCACAGCTGGACATCGCCGAGAAGCGCGTCCCGCAGGACGG
CCGCATCAAGCTCAACCTGTCCAAGACCAAGCAGATCGACTTCCGCGTCAGCACGCTGCCGACCCTGTTCGGCGAGAAGG
TGGTGCTGCGTATCCTCGATGGCAGCGCGGCCAAGCTGGGCATCGACAAGCTGGGTTACGAGCCGGACCAGCAGAAGCTG
TTCCTGGATGCCATCCACAAGCCCTACGGCATGGTGCTGGTGACCGGCCCGACCGGATCGGGCAAGACCGTGTCGCTGTA
TACCGCGCTGGGTATCCTCAACGACGAGACGCGCAACATCTCCACTGCCGAAGACCCGGTGGAAATCCGCCTGCCCGGCG
TCAACCAGGTACAGCAGAACAACAAGCGCGGCATGACCTTCGCCGCGGCGCTGCGCTCGTTCCTGCGACAGGACCCGGAC
ATCATCATGGTCGGCGAAATCCGCGACCTGGAAACGGCGGAGATCGCGATCAAGGCCGCGCAGACCGGCCACATGGTGCT
GTCCACGCTGCATACCAACGATGCGCCGCAGACCATCGCGCGCCTGATGAACATGGGTATCGCGCCCTACAACATCACCA
GCTCGGTGACGCTGGTGATCGCCCAGCGCCTGGCGCGTCGCCTGTGCGGCAACTGCAAGCGTCCGTCCAGCCTGCCCGAT
CATGCGCTGCTGGCCGAAGGCTTCACGCAGGCGCAGCTGGATGCGGGCATCGAGCTGTACGAGGCCGTGGGCTGCGATGA
ATGCACCGAGGGCTACAAGGGCCGGACCGGCGTCTACCAGGTCATGCCGATGACCGATGAGATCGCCACCATCGTGCTGG
CCGGTGGCAATGCGCTGCAGATTGCCGAGGCGGCGCAGCAGATCGGGGTGAATGACCTGCGCCAGTCGGCATTGAAGAAG
GCGGCGGCGGGCATCACCAGCCTGGCCGAGATCAACCGCGTCACCAAGGATTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(50-150)

Type II/IV secretion system protein (T2SSE)

(200-467)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

55.25

100

0.556
  pilB Acinetobacter baylyi ADP1

54.53

100

0.553
  pilB Legionella pneumophila strain ERS1305867

51.93

98.787

0.513
  pilB Vibrio cholerae strain A1552

49.206

98.267

0.484
  pilF Neisseria gonorrhoeae MS11

48.772

98.787

0.482
  pilB Vibrio parahaemolyticus RIMD 2210633

45.629

99.133

0.452
  pilB Vibrio campbellii strain DS40M4

45.518

98.614

0.449

Multiple sequence alignment