Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   EEY24_RS04385 Genome accession   NZ_CP033575
Coordinates   729995..731701 (-) Length   568 a.a.
NCBI ID   WP_123115558.1    Uniprot ID   -
Organism   Shewanella algae strain KC-Na-R1     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 724995..736701
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EEY24_RS04355 (EEY24_04350) mutT 725585..725983 (-) 399 WP_025012058.1 8-oxo-dGTP diphosphatase MutT -
  EEY24_RS04360 (EEY24_04355) yacG 725983..726195 (-) 213 WP_025886789.1 DNA gyrase inhibitor YacG -
  EEY24_RS04365 (EEY24_04360) zapD 726240..726980 (-) 741 WP_028779562.1 cell division protein ZapD -
  EEY24_RS04370 (EEY24_04365) coaE 726977..727588 (-) 612 WP_123115556.1 dephospho-CoA kinase -
  EEY24_RS04375 (EEY24_04370) pilD 727589..728503 (-) 915 WP_123115557.1 A24 family peptidase Machinery gene
  EEY24_RS04380 (EEY24_04375) pilC 728579..729844 (-) 1266 WP_107111642.1 type II secretion system F family protein Machinery gene
  EEY24_RS04385 (EEY24_04380) pilB 729995..731701 (-) 1707 WP_123115558.1 type IV-A pilus assembly ATPase PilB Machinery gene
  EEY24_RS04390 (EEY24_04385) - 731762..732193 (-) 432 WP_123115559.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  EEY24_RS04395 (EEY24_04390) nadC 733061..733921 (-) 861 WP_123115560.1 carboxylating nicotinate-nucleotide diphosphorylase -
  EEY24_RS04400 (EEY24_04395) ampD 734113..734658 (+) 546 WP_240647823.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  EEY24_RS04405 (EEY24_04400) ampE 734712..735563 (+) 852 WP_025012050.1 beta-lactamase regulator AmpE -
  EEY24_RS04410 (EEY24_04405) pdhR 735839..736591 (+) 753 WP_025886793.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -

Sequence

Protein


Download         Length: 568 a.a.        Molecular weight: 63019.12 Da        Isoelectric Point: 5.2529

>NTDB_id=323998 EEY24_RS04385 WP_123115558.1 729995..731701(-) (pilB) [Shewanella algae strain KC-Na-R1]
MPTSGLHLGLSTLFIRKGLLDESSLSEAIKQSQKNKQTLVTTLVTSQLLSARTIAELCYEEYGTPLLDLNEFDLSSIPEE
FLNKKLIDKHRCLPLFKRGNRLYIATSDPTNIAALEDFQFSAGLHAEAILVEEDKLSKALEKVLEEDLSSLDLDGLDEES
LAGIEVSDTDKRQQEENEGKDDAPIVIYINKILTDAIRKGASDLHFEPYEKRYRIRFRIDGILHEVSEPPISLSGRISAR
LKVMSKLDIAERRVPQDGRIKMKLSRNKSIDFRVSTLPTLWGEKIVMRILDSSSAQLGIEKLGYEEDQKQAYLEMLARPQ
GMILVTGPTGSGKTVSLYTGLNILNTEERNISTAEDPVEINLEGVNQVHINTKAGLTFASALRSFLRQDPDVVMVGEIRD
LETAEIAIKAAQTGHLVLSTLHTNSSAETLTRLLNMGVPGYNIASSVNLIIAQRLARRLCPECRQPEDIPEHELLQLGFT
QQQINAGFTPYKPCGCDLCSGGYKGRVGIYEVMKMSDEIARTIMEGGNSLQIATTAKQQGMRDLRLSGLLKVIQGVTSIA
EINRVTSY

Nucleotide


Download         Length: 1707 bp        

>NTDB_id=323998 EEY24_RS04385 WP_123115558.1 729995..731701(-) (pilB) [Shewanella algae strain KC-Na-R1]
ATGCCAACATCAGGCCTTCACTTAGGATTGTCCACTCTGTTTATCCGTAAGGGACTGCTCGACGAGAGTAGTCTGTCGGA
GGCCATCAAGCAGTCGCAAAAGAACAAGCAAACACTGGTCACCACCTTGGTGACCAGTCAGCTTTTATCCGCCCGTACCA
TCGCCGAACTCTGCTATGAAGAGTATGGCACCCCGCTGCTGGATCTCAATGAGTTTGATTTAAGCAGTATCCCGGAAGAG
TTTCTCAATAAAAAGCTAATCGACAAGCACCGCTGCCTGCCGTTATTTAAGCGTGGCAACCGCCTCTATATCGCCACTTC
TGACCCCACCAATATCGCCGCCCTGGAGGACTTTCAGTTCAGCGCAGGCCTGCACGCCGAAGCGATACTGGTAGAGGAAG
ATAAGCTCAGTAAAGCGCTGGAAAAGGTGCTTGAAGAGGACTTGAGTTCACTGGACCTGGATGGCCTCGATGAGGAATCC
CTCGCCGGTATTGAAGTCAGCGATACCGACAAACGTCAACAGGAAGAAAATGAAGGCAAAGACGATGCGCCTATCGTCAT
CTATATCAATAAAATTTTGACCGATGCCATCCGCAAGGGCGCATCGGATCTGCATTTCGAGCCCTACGAAAAACGCTACC
GCATCCGTTTTCGTATTGATGGCATCCTCCACGAAGTATCTGAGCCACCAATTTCCCTCTCAGGCCGTATCTCTGCCCGC
TTGAAGGTAATGTCCAAACTGGATATCGCCGAGCGCCGGGTCCCTCAGGATGGCCGCATCAAGATGAAGCTGTCCCGCAA
CAAGAGTATCGATTTCCGGGTAAGTACCCTGCCCACCCTCTGGGGTGAAAAGATAGTGATGCGGATCCTAGACTCCTCTT
CCGCGCAATTAGGCATCGAAAAACTCGGCTATGAGGAAGACCAAAAACAAGCCTATCTGGAGATGCTGGCAAGGCCCCAG
GGAATGATCTTGGTAACCGGCCCTACCGGCTCGGGCAAAACCGTGTCCCTTTATACTGGCCTCAATATCCTCAATACCGA
AGAGCGCAATATCAGCACCGCCGAAGACCCGGTGGAGATCAACCTGGAAGGGGTCAACCAGGTACATATCAACACCAAGG
CCGGATTAACCTTCGCCTCGGCGCTGCGCTCCTTTTTGCGTCAGGACCCGGATGTGGTGATGGTGGGCGAAATTCGCGAC
CTGGAAACCGCCGAAATCGCCATTAAGGCGGCGCAAACCGGCCACTTGGTACTCTCGACCCTGCATACCAATTCATCGGC
CGAAACTCTCACCCGCCTGCTCAATATGGGTGTCCCAGGTTATAACATTGCCAGTTCGGTCAACCTGATTATTGCCCAGC
GCCTGGCCCGCCGCCTTTGCCCCGAATGCCGCCAACCGGAAGATATCCCCGAGCATGAACTGCTGCAACTGGGCTTCACT
CAGCAGCAGATAAACGCAGGCTTTACCCCTTACAAGCCCTGTGGCTGCGATCTCTGCTCCGGTGGCTATAAGGGCCGGGT
CGGTATCTATGAGGTAATGAAGATGTCCGATGAAATCGCCAGAACCATTATGGAGGGCGGTAATTCGCTGCAGATTGCCA
CCACCGCCAAACAGCAAGGGATGCGCGATCTGCGCCTTTCCGGCCTTTTAAAGGTGATTCAAGGCGTCACCAGTATCGCC
GAGATAAATCGGGTCACCAGCTATTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(191-458)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(43-145)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

58.741

100

0.592
  pilB Acinetobacter baylyi ADP1

59.358

98.768

0.586
  pilB Legionella pneumophila strain ERS1305867

56.184

99.648

0.56
  pilB Vibrio parahaemolyticus RIMD 2210633

52.557

99.824

0.525
  pilB Vibrio cholerae strain A1552

55.344

92.254

0.511
  pilB Vibrio campbellii strain DS40M4

51.332

99.12

0.509
  pilF Neisseria gonorrhoeae MS11

47.611

99.472

0.474
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.068

98.239

0.384
  pilF Thermus thermophilus HB27

37.102

99.648

0.37

Multiple sequence alignment