Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   EEY24_RS04375 Genome accession   NZ_CP033575
Coordinates   727589..728503 (-) Length   304 a.a.
NCBI ID   WP_123115557.1    Uniprot ID   -
Organism   Shewanella algae strain KC-Na-R1     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 722589..733503
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EEY24_RS04355 (EEY24_04350) mutT 725585..725983 (-) 399 WP_025012058.1 8-oxo-dGTP diphosphatase MutT -
  EEY24_RS04360 (EEY24_04355) yacG 725983..726195 (-) 213 WP_025886789.1 DNA gyrase inhibitor YacG -
  EEY24_RS04365 (EEY24_04360) zapD 726240..726980 (-) 741 WP_028779562.1 cell division protein ZapD -
  EEY24_RS04370 (EEY24_04365) coaE 726977..727588 (-) 612 WP_123115556.1 dephospho-CoA kinase -
  EEY24_RS04375 (EEY24_04370) pilD 727589..728503 (-) 915 WP_123115557.1 A24 family peptidase Machinery gene
  EEY24_RS04380 (EEY24_04375) pilC 728579..729844 (-) 1266 WP_107111642.1 type II secretion system F family protein Machinery gene
  EEY24_RS04385 (EEY24_04380) pilB 729995..731701 (-) 1707 WP_123115558.1 type IV-A pilus assembly ATPase PilB Machinery gene
  EEY24_RS04390 (EEY24_04385) - 731762..732193 (-) 432 WP_123115559.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -

Sequence

Protein


Download         Length: 304 a.a.        Molecular weight: 33885.30 Da        Isoelectric Point: 7.2147

>NTDB_id=323996 EEY24_RS04375 WP_123115557.1 727589..728503(-) (pilD) [Shewanella algae strain KC-Na-R1]
MSEFIAVLSHNQWLFLVLCFLFASVIGSFLNVVIHRLPIMMKREWQQECNQYLQEYQPEFAKQLGEAKLNAPIDNYPAKY
NLILPKSACPKCGAHIKPWHNLPIIGYLLLGGKCASCNTAISARYPIFELLTALLISTLGWYFGPTLQFVYATILTFGLI
ALSGIDLDEMLLPDQITLPLLWLGLLVNISGTFTNLTDATIGAAAGYLSLWSVYWLFKLLTGKEGMGYGDFKLMALFGAW
LGWQMLPLIILLSSLVGALVGVLLILFKNLNRGNPIPFGPYIAIAGWVALMWGQQIVDWYLGTL

Nucleotide


Download         Length: 915 bp        

>NTDB_id=323996 EEY24_RS04375 WP_123115557.1 727589..728503(-) (pilD) [Shewanella algae strain KC-Na-R1]
ATGTCAGAATTTATTGCCGTATTGAGCCACAACCAGTGGCTCTTTCTTGTTCTCTGTTTTTTGTTTGCCTCAGTGATAGG
CAGCTTTCTCAATGTGGTGATCCACCGCCTGCCGATAATGATGAAGCGCGAATGGCAGCAGGAATGCAATCAATACCTGC
AGGAATATCAACCCGAATTCGCCAAACAGCTGGGTGAAGCCAAGCTTAATGCACCAATAGATAACTACCCGGCCAAGTAC
AATCTGATACTGCCGAAGTCTGCCTGCCCCAAGTGTGGCGCCCATATCAAACCCTGGCACAACCTCCCCATAATCGGCTA
TTTGTTGCTTGGCGGTAAATGCGCCTCCTGCAACACAGCTATTTCAGCGAGATACCCGATATTTGAGCTACTGACCGCCC
TGCTTATCAGTACTCTCGGTTGGTATTTTGGCCCGACACTGCAGTTTGTTTACGCCACTATTCTCACCTTTGGATTGATA
GCCCTCAGCGGGATAGATTTGGATGAAATGCTGCTGCCGGATCAGATTACCCTGCCGCTACTGTGGCTTGGTTTACTGGT
TAATATTTCGGGCACCTTTACTAATCTGACCGATGCCACCATAGGGGCCGCTGCCGGCTATTTAAGTCTCTGGTCGGTCT
ACTGGCTATTTAAACTGCTCACCGGCAAGGAAGGCATGGGCTATGGCGACTTCAAGCTGATGGCACTGTTTGGCGCCTGG
CTTGGCTGGCAGATGTTACCTCTAATTATTCTGCTCTCTTCTCTGGTCGGTGCCCTAGTAGGCGTGTTACTCATTTTGTT
CAAGAACCTTAATCGGGGTAACCCTATCCCCTTTGGCCCTTATATTGCTATCGCAGGTTGGGTCGCTCTGATGTGGGGGC
AACAGATTGTCGACTGGTATTTGGGAACACTCTAA

Domains

Predicted by InterproScan.

Type IV leader peptidase family (Peptidase_A24)

(154-262)

Bacterial Peptidase A24 N-terminal domain (A24_N_bact)

(21-143)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Vibrio campbellii strain DS40M4

49.169

99.013

0.487
  pilD Vibrio cholerae strain A1552

50

96.053

0.48
  pilD Acinetobacter nosocomialis M2

48.013

99.342

0.477
  pilD Acinetobacter baumannii D1279779

47.682

99.342

0.474
  pilD Neisseria gonorrhoeae MS11

44.84

92.434

0.414

Multiple sequence alignment