Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   CDG68_RS20305 Genome accession   NZ_CP033133
Coordinates   3624612..3626336 (+) Length   574 a.a.
NCBI ID   WP_087552281.1    Uniprot ID   A0A3G2T759
Organism   Acinetobacter wuhouensis strain WCHAW010062     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3619612..3631336
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CDG68_RS20265 (CDG68_20270) nusA 3620380..3621864 (-) 1485 WP_068973839.1 transcription termination factor NusA -
  CDG68_RS20270 (CDG68_20275) rimP 3621899..3622423 (-) 525 WP_087552284.1 ribosome maturation factor RimP -
  CDG68_RS20295 (CDG68_20300) secG 3623222..3623554 (-) 333 WP_087552283.1 preprotein translocase subunit SecG -
  CDG68_RS20300 (CDG68_20305) tpiA 3623564..3624358 (-) 795 WP_087552282.1 triose-phosphate isomerase -
  CDG68_RS20305 (CDG68_20310) pilB 3624612..3626336 (+) 1725 WP_087552281.1 type IV-A pilus assembly ATPase PilB Machinery gene
  CDG68_RS20310 (CDG68_20315) pilC 3626369..3627595 (+) 1227 WP_087552280.1 type II secretion system F family protein Machinery gene
  CDG68_RS20315 (CDG68_20320) pilD 3627595..3628455 (+) 861 WP_087552279.1 prepilin peptidase Machinery gene
  CDG68_RS20320 (CDG68_20325) coaE 3628494..3629105 (+) 612 WP_087552278.1 dephospho-CoA kinase -
  CDG68_RS20325 (CDG68_20330) - 3629118..3630035 (-) 918 WP_087552277.1 DMT family transporter -
  CDG68_RS20330 (CDG68_20335) rlmB 3630092..3630844 (-) 753 WP_068973848.1 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB -

Sequence

Protein


Download         Length: 574 a.a.        Molecular weight: 64306.01 Da        Isoelectric Point: 5.8372

>NTDB_id=321666 CDG68_RS20305 WP_087552281.1 3624612..3626336(+) (pilB) [Acinetobacter wuhouensis strain WCHAW010062]
MADDELKSKYPTYLDRLVTNGFLTNEQIHRMFYEINKNNKLIVQYLVEAFDIKPLIIAEAISAEYEEPLLDLSTFNLSLI
PKGAIDEKLITKHKVIPLSVKDHSLFIAVADPTLTHDLDTIRFNSKKKLEIIIVEHDKLERALEEIYGTNAQLEFLNNLH
NNDDSENTEDILKELNFDINADHEKDDTIVVKYLNKILYDAIKMGASDLHFEPYAKIYRIRYRIDGVLHSISFPPLVLAP
RLTVRLKIMSKMDISERRSPQDGRIKLPVSQFKSIDFRVSSLPTLYGEKLVLRILDASSATLGIDALGYEEDQKALFLEA
LNKPQGMLLITGPTGSGKTVSLYTGLNILNTESSNISTAEDPVEINLEGINQVNVNPKVGLTFSAALKSFLRQDPDIIMV
GEIRDLETAEIAIKAAQTGHMVMSTLHTNSAPETLTRLHNMGVASFNLATSINLVIAQRLARRLCNKCKKPIQIPRNSLI
ELGFTEQDLSSSKFTVYQPVGCPECREGYKGRVGIYEVMKVTPEISKIIMEDGNALEIAAASEKSGFNNLRRSGLIKVMN
GLTSLQEVNRVTSE

Nucleotide


Download         Length: 1725 bp        

>NTDB_id=321666 CDG68_RS20305 WP_087552281.1 3624612..3626336(+) (pilB) [Acinetobacter wuhouensis strain WCHAW010062]
ATGGCGGATGATGAACTAAAGTCAAAATATCCAACCTATTTGGATCGTTTAGTAACGAATGGATTTCTGACAAATGAACA
GATTCATCGAATGTTTTATGAAATAAATAAAAACAACAAACTCATAGTTCAATACTTAGTAGAAGCTTTTGATATAAAAC
CATTAATCATTGCAGAGGCTATTTCTGCTGAATATGAAGAACCTCTACTTGATCTTAGCACATTTAATCTTTCGCTAATT
CCAAAAGGCGCAATCGATGAAAAATTAATCACAAAACATAAAGTTATACCTTTAAGTGTTAAAGACCACTCATTATTTAT
TGCAGTAGCCGACCCCACTCTCACACATGATCTAGATACCATTCGATTTAATAGCAAGAAAAAACTTGAGATTATTATCG
TTGAACATGACAAACTAGAACGAGCTCTCGAAGAGATTTACGGCACAAATGCTCAGCTTGAATTTCTTAATAATCTTCAC
AATAATGATGATAGCGAAAACACTGAAGACATATTAAAAGAGCTCAACTTTGATATAAATGCTGATCATGAAAAAGACGA
TACTATCGTTGTTAAATATTTAAATAAAATACTTTATGATGCTATAAAAATGGGGGCATCTGATCTTCACTTTGAACCAT
ATGCCAAGATTTACCGTATTAGATACCGTATTGATGGTGTGTTACATTCAATCTCGTTTCCTCCTTTAGTTCTTGCACCT
AGACTTACCGTCCGACTAAAAATCATGTCGAAGATGGATATATCTGAACGTCGCTCACCACAAGATGGTCGTATCAAGTT
ACCAGTTTCACAGTTTAAATCCATAGATTTTCGTGTTAGCTCATTACCAACTTTATATGGTGAAAAACTAGTTTTACGTA
TCTTAGATGCATCAAGTGCTACATTAGGTATCGACGCGCTAGGTTACGAGGAAGACCAAAAAGCACTTTTTTTAGAAGCT
CTAAACAAACCACAAGGTATGTTATTAATTACTGGACCAACAGGTTCAGGGAAAACCGTTTCGCTTTATACTGGCTTAAA
TATTTTGAACACCGAAAGTTCGAATATTTCAACTGCCGAAGATCCTGTCGAAATTAATCTTGAAGGTATCAATCAAGTTA
ACGTCAACCCAAAAGTAGGATTAACATTTTCAGCCGCCTTGAAATCTTTTTTACGTCAAGATCCTGATATCATCATGGTC
GGGGAGATTCGAGACCTTGAAACGGCTGAAATTGCGATTAAAGCTGCACAAACAGGTCATATGGTGATGTCTACACTACA
TACCAATAGTGCACCTGAAACATTAACGAGATTGCATAATATGGGTGTGGCATCATTTAATCTTGCTACATCTATCAACT
TGGTAATCGCTCAGCGCCTTGCCAGACGACTATGCAATAAGTGTAAAAAACCGATTCAAATTCCACGAAATAGCTTAATT
GAGTTGGGATTTACAGAACAAGATTTATCTAGTTCAAAATTTACTGTTTATCAACCAGTAGGTTGTCCAGAATGCAGAGA
AGGCTATAAAGGCCGTGTAGGTATTTATGAAGTAATGAAAGTCACGCCTGAAATTTCGAAGATTATTATGGAAGATGGTA
ATGCGCTTGAAATCGCAGCAGCATCTGAAAAATCGGGGTTTAATAATTTAAGACGCTCAGGCTTAATTAAAGTGATGAAT
GGTTTAACTTCTTTACAAGAAGTCAATCGTGTTACAAGTGAATAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(196-463)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(17-151)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A3G2T759

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

66.898

100

0.672
  pilB Acinetobacter baylyi ADP1

66.378

100

0.667
  pilB Legionella pneumophila strain ERS1305867

51.407

92.857

0.477
  pilB Vibrio parahaemolyticus RIMD 2210633

47.452

99.129

0.47
  pilF Neisseria gonorrhoeae MS11

47.95

97.735

0.469
  pilB Vibrio cholerae strain A1552

49.712

90.767

0.451
  pilB Vibrio campbellii strain DS40M4

52.567

84.843

0.446
  pilB/pilB1 Synechocystis sp. PCC 6803

33.548

100

0.362

Multiple sequence alignment