Detailed information NTG0321668

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: CDG68_RS20315
• Genome accession: NZ_CP033133
• Coordinates: 3627595..3628455 (+)
• Length: 286 a.a.
• NCBI ID: WP_087552279.1
• Uniprot ID: A0A3G2T877
• Organism: Acinetobacter wuhouensis strain WCHAW010062
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3622595..3633455

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
CDG68_RS20295 (CDG68_20300)	secG	3623222..3623554 (-)	333	WP_087552283.1	preprotein translocase subunit SecG	-
CDG68_RS20300 (CDG68_20305)	tpiA	3623564..3624358 (-)	795	WP_087552282.1	triose-phosphate isomerase	-
CDG68_RS20305 (CDG68_20310)	pilB	3624612..3626336 (+)	1725	WP_087552281.1	type IV-A pilus assembly ATPase PilB	Machinery gene
CDG68_RS20310 (CDG68_20315)	pilC	3626369..3627595 (+)	1227	WP_087552280.1	type II secretion system F family protein	Machinery gene
CDG68_RS20315 (CDG68_20320)	pilD	3627595..3628455 (+)	861	WP_087552279.1	prepilin peptidase	Machinery gene
CDG68_RS20320 (CDG68_20325)	coaE	3628494..3629105 (+)	612	WP_087552278.1	dephospho-CoA kinase	-
CDG68_RS20325 (CDG68_20330)	-	3629118..3630035 (-)	918	WP_087552277.1	DMT family transporter	-
CDG68_RS20330 (CDG68_20335)	rlmB	3630092..3630844 (-)	753	WP_068973848.1	23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB	-
CDG68_RS20335 (CDG68_20340)	-	3631022..3631345 (-)	324	WP_068973849.1	pyrimidine/purine nucleoside phosphorylase	-
CDG68_RS20340 (CDG68_20345)	-	3631429..3632739 (-)	1311	WP_087552276.1	tetratricopeptide repeat protein	-

Sequence

Protein

• Length: 286 a.a.
• Molecular weight: 32008.34 Da
• Isoelectric Point: 7.4699

>NTDB_id=321668 CDG68_RS20315 WP_087552279.1 3627595..3628455(+) (pilD) [Acinetobacter wuhouensis strain WCHAW010062]
MQELLDYLIQTPTALYLTVGVFSLCIGSFLNVVIYRTPKMMEQEWHTDCQLLLHPEQPIIDEEKLSLSKPASTCPKCKTS
IRWYQNIPVISWLALKGKCGTCQNPISIRYPFIELLTAICSLIVVAVFGPTLQMVFGVFLTWVLITLTFIDFDTQLLPDR
FTLTLGAIGLGINSFNIYTTATSAIWGYIIGFLCLWIVYYLFKVVTGKEGMGYGDFKLLAALGAWMGPLLLPLIILLSSV
VGAIIGIILLKIRKENIPFAFGPYIAIAGWIAFLWGDQIMKIYLGQ

Nucleotide

• Length: 861 bp

>NTDB_id=321668 CDG68_RS20315 WP_087552279.1 3627595..3628455(+) (pilD) [Acinetobacter wuhouensis strain WCHAW010062]
ATGCAAGAACTTCTCGATTATTTGATTCAAACACCAACGGCACTTTATTTGACTGTTGGTGTGTTTAGTTTGTGCATTGG
TAGTTTTTTAAATGTTGTCATTTACAGAACACCCAAAATGATGGAACAAGAGTGGCATACTGACTGTCAACTTCTGCTTC
ATCCAGAGCAGCCCATCATTGATGAAGAAAAACTCAGTTTAAGCAAACCAGCTTCAACATGCCCAAAATGCAAAACCTCA
ATTCGTTGGTATCAAAATATACCTGTTATTAGTTGGTTAGCATTAAAAGGAAAATGTGGTACTTGCCAAAACCCAATCAG
CATTCGTTATCCTTTTATTGAGTTACTGACTGCTATATGCTCTTTAATTGTCGTTGCTGTTTTTGGACCAACTTTACAAA
TGGTTTTCGGGGTCTTTTTAACATGGGTGCTGATTACCCTCACCTTTATTGATTTTGATACCCAACTGCTCCCAGATCGC
TTCACTCTGACACTTGGTGCGATTGGCTTAGGCATTAACAGTTTTAACATATACACCACTGCAACATCAGCAATCTGGGG
CTATATTATTGGTTTTCTATGCTTATGGATTGTCTACTACCTCTTTAAAGTTGTGACTGGCAAAGAAGGCATGGGCTATG
GTGATTTTAAATTACTTGCAGCATTGGGTGCATGGATGGGACCATTACTCCTCCCATTAATTATTCTATTGTCATCAGTT
GTAGGCGCGATTATTGGAATTATTCTCCTCAAAATACGCAAGGAAAATATTCCTTTCGCATTTGGTCCTTATATCGCGAT
AGCAGGTTGGATTGCTTTCTTATGGGGCGATCAAATCATGAAGATTTATTTAGGACAATAA

Domains

Predicted by InterproScan.

• Type IV leader peptidase family (Peptidase_A24) (139-247)

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (21-127)

3D structure

• AlphaFold DB: A0A3G2T877

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Acinetobacter baumannii D1279779	79.298	99.65	0.79
pilD	Acinetobacter nosocomialis M2	79.298	99.65	0.79
pilD	Vibrio cholerae strain A1552	55.224	93.706	0.517
pilD	Vibrio campbellii strain DS40M4	54.682	93.357	0.51
pilD	Neisseria gonorrhoeae MS11	44.604	97.203	0.434