Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   EAW52_RS18245 Genome accession   NZ_CP033105
Coordinates   3922945..3925065 (-) Length   706 a.a.
NCBI ID   WP_162997778.1    Uniprot ID   -
Organism   Pseudomonas sp. LTJR-52     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3917945..3930065
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EAW52_RS18230 (EAW52_18230) - 3919653..3921257 (-) 1605 WP_122043467.1 SPOR domain-containing protein -
  EAW52_RS18235 (EAW52_18235) aroB 3921268..3922371 (-) 1104 WP_122043468.1 3-dehydroquinate synthase -
  EAW52_RS18240 (EAW52_18240) aroK 3922421..3922939 (-) 519 WP_026003701.1 shikimate kinase AroK -
  EAW52_RS18245 (EAW52_18245) pilQ 3922945..3925065 (-) 2121 WP_162997778.1 type IV pilus secretin PilQ Machinery gene
  EAW52_RS18250 (EAW52_18250) pilP 3925078..3925602 (-) 525 WP_122043470.1 type 4a pilus biogenesis lipoprotein PilP -
  EAW52_RS18255 (EAW52_18255) pilO 3925599..3926222 (-) 624 WP_019363987.1 type 4a pilus biogenesis protein PilO -
  EAW52_RS18260 (EAW52_18260) - 3926219..3926806 (-) 588 WP_122043471.1 PilN domain-containing protein -
  EAW52_RS18265 (EAW52_18265) comM 3926806..3927870 (-) 1065 WP_019363985.1 pilus assembly protein PilM Machinery gene

Sequence

Protein


Download         Length: 706 a.a.        Molecular weight: 75909.24 Da        Isoelectric Point: 5.8201

>NTDB_id=321421 EAW52_RS18245 WP_162997778.1 3922945..3925065(-) (pilQ) [Pseudomonas sp. LTJR-52]
MLHKTILQRVSGKSALTLAILLSGICPSLLLAADLKAMNVASLPGDRVELKLSFDQPVTAPKGYTIEQPARIALDLPGVN
NKLGVRNKELGSGNARSVTVVEAGDRTRLIVNLTALAPYSTRVQGNDLFVVIGGDQAARTTSASVPAPSLPRSGAAQAPA
PATNPAGRVFSSVPRLAGKAIRNIDFKRGDDGEGNVIIDLSETGISPDIQEQGGKIRLNFGKTQLPDALRVRLDVKDFAT
PVQFVNASSQAGDTVITIEPTGVYDYLAYQTDNKLTISVKPLTAKEKEQRAAERFTYTGEKLSLNFQDIDVRSVLQLIAD
FTGLNLVASDTVQGNITLRLQNVPWDQALDLVLKTKGLDKRQIGNVLLIAPADEIAARERQELESQKQIQELAPLRRELI
QVNYAKAADMAKLFQSVTKADAGQSERGSITVDDRTNSIIAYQTQERLDELRRIVSQLDIPVRQVMIQARIVEANVNYNK
QLGVNWSGSRTGNGNFTLSGAAASGTSTSTPFVDLGASSPTSTLGIGFLTSNTTLDLELSAMEASGNGEIVSQPKVVTSD
KETAKILRGTEIPYQEASSSGATSVSFKEAALSLEVTPQITPDNRIIMEVKVNKDAPDYTNEVNGVPPITKNEVNAKVLI
ADGETIVIGGVFSNTQSKSTSKVPLLGDVPYVGRLFRRDEITDEKVELLVFLTPRIMNNQAIAVGR

Nucleotide


Download         Length: 2121 bp        

>NTDB_id=321421 EAW52_RS18245 WP_162997778.1 3922945..3925065(-) (pilQ) [Pseudomonas sp. LTJR-52]
ATGTTGCACAAAACTATTCTACAAAGGGTCTCAGGGAAGAGTGCCCTCACGCTGGCCATACTCTTGAGCGGAATCTGTCC
CTCTTTGCTGCTGGCGGCAGATCTCAAGGCCATGAATGTGGCCTCTTTGCCGGGCGACAGAGTCGAACTCAAACTATCGT
TCGATCAGCCAGTAACAGCCCCCAAAGGCTATACCATTGAGCAACCTGCGCGCATCGCTTTGGATCTGCCTGGCGTTAAT
AACAAGCTAGGCGTTCGCAACAAGGAGTTGGGGTCGGGGAATGCCAGAAGCGTTACCGTAGTCGAGGCGGGTGACCGCAC
GCGTCTTATCGTGAATCTGACTGCATTGGCGCCTTACTCGACCCGCGTTCAGGGTAACGACCTGTTCGTTGTCATTGGCG
GCGACCAGGCTGCTCGCACGACTAGTGCTTCTGTCCCGGCACCATCCTTGCCGCGCTCCGGTGCTGCGCAAGCCCCAGCG
CCTGCAACCAATCCTGCCGGCCGTGTCTTTTCCAGCGTGCCGAGATTGGCTGGGAAGGCCATCAGGAATATTGACTTCAA
GCGAGGTGATGACGGAGAAGGTAACGTTATCATTGACCTTTCAGAAACAGGCATCAGCCCTGACATACAGGAGCAGGGTG
GAAAGATTCGCCTTAACTTCGGCAAGACGCAGCTCCCTGATGCTCTACGTGTTCGGCTTGACGTCAAAGACTTTGCTACG
CCAGTACAGTTTGTAAACGCCTCAAGTCAGGCTGGTGACACGGTCATTACAATTGAGCCTACAGGCGTATATGACTACTT
GGCTTATCAGACGGATAACAAGCTGACCATTAGCGTCAAGCCGCTGACTGCCAAGGAAAAGGAACAACGTGCCGCTGAGC
GTTTCACCTATACCGGTGAAAAACTGTCTCTGAATTTCCAGGATATCGATGTTCGCTCGGTCCTTCAGTTAATTGCTGAC
TTCACCGGTTTGAACCTGGTGGCCAGTGACACTGTACAAGGCAACATCACGCTGCGCCTGCAAAACGTGCCGTGGGATCA
GGCGCTTGACCTTGTTCTGAAAACCAAGGGACTCGACAAGCGACAAATAGGCAACGTGCTGTTGATTGCACCAGCGGATG
AGATTGCGGCGCGTGAACGCCAAGAATTGGAGTCACAAAAGCAGATTCAGGAGCTGGCGCCGCTACGCAGAGAGCTGATC
CAGGTTAACTACGCTAAGGCCGCCGATATGGCTAAGCTCTTCCAGTCGGTCACTAAGGCTGATGCGGGGCAAAGCGAGCG
TGGCTCCATTACCGTTGACGATCGGACAAACAGCATCATTGCCTATCAAACCCAGGAACGCCTAGATGAACTAAGACGGA
TTGTCTCCCAGCTCGACATCCCGGTTCGTCAAGTGATGATCCAAGCGAGGATTGTCGAGGCTAATGTGAATTATAATAAG
CAGTTAGGTGTTAATTGGTCGGGATCACGTACAGGGAATGGAAATTTTACACTTAGCGGTGCTGCTGCCTCTGGTACTAG
TACAAGTACTCCTTTTGTCGATTTAGGTGCAAGCAGCCCAACCTCAACATTGGGTATCGGTTTCCTGACGAGCAACACCA
CATTAGATCTTGAACTTTCTGCTATGGAAGCAAGCGGTAATGGAGAAATTGTTTCGCAGCCCAAGGTAGTGACTTCTGAT
AAGGAAACAGCAAAGATTCTACGTGGTACAGAAATTCCTTATCAGGAAGCTAGCTCTAGTGGTGCAACAAGTGTTTCTTT
TAAAGAGGCAGCTCTGTCATTAGAGGTAACACCTCAAATTACGCCTGATAATAGAATTATCATGGAAGTTAAAGTTAATA
AGGATGCGCCTGATTACACCAATGAGGTTAATGGCGTACCTCCAATTACAAAAAACGAGGTAAACGCCAAAGTTCTAATA
GCTGATGGAGAAACCATTGTTATTGGTGGCGTATTTTCAAATACGCAAAGCAAGTCGACAAGCAAGGTCCCTTTACTAGG
TGACGTTCCTTACGTAGGCCGCCTTTTCCGTCGTGATGAAATTACTGATGAAAAAGTAGAGCTGCTTGTCTTCCTCACTC
CGCGTATCATGAACAACCAAGCTATTGCCGTTGGTCGTTGA

Domains

Predicted by InterproScan.

Bacterial type II and III secretion system protein (Secretin)

(541-697)

Secretin and TonB N terminus short domain (STN)

(325-371)

AMIN domain (AMIN)

(47-131)

Bacterial type II/III secretion system short domain (Secretin_N)

(399-464)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

71.961

100

0.738
  comQ Acinetobacter baylyi ADP1

40.423

100

0.407
  pilQ Acinetobacter baumannii strain A118

39.861

100

0.407
  pilQ Acinetobacter baumannii D1279779

39.861

100

0.407
  pilQ Legionella pneumophila strain Lp02

39.385

96.742

0.381
  pilQ Legionella pneumophila strain ERS1305867

39.239

96.742

0.38

Multiple sequence alignment