Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   EAE30_RS17175 Genome accession   NZ_CP033078
Coordinates   2587309..2588637 (-) Length   442 a.a.
NCBI ID   WP_164711885.1    Uniprot ID   -
Organism   Vibrio zhugei strain HBUAS61001     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2582309..2593637
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EAE30_RS17155 - 2583057..2583887 (-) 831 WP_123017009.1 Dam family site-specific DNA-(adenine-N6)-methyltransferase -
  EAE30_RS17160 - 2583958..2585439 (-) 1482 WP_123017010.1 AAA family ATPase -
  EAE30_RS17165 aroB 2585492..2586571 (-) 1080 WP_123017011.1 3-dehydroquinate synthase -
  EAE30_RS17170 aroK 2586603..2587121 (-) 519 WP_077314121.1 shikimate kinase AroK -
  EAE30_RS17175 pilQ 2587309..2588637 (-) 1329 WP_164711885.1 type IV pilus secretin PilQ Machinery gene
  EAE30_RS17180 - 2588627..2589118 (-) 492 WP_123017013.1 hypothetical protein -
  EAE30_RS17185 pilO 2589111..2589683 (-) 573 WP_123017014.1 type 4a pilus biogenesis protein PilO -
  EAE30_RS17190 - 2589667..2590227 (-) 561 WP_123017015.1 hypothetical protein -
  EAE30_RS17195 pilM 2590220..2591173 (-) 954 WP_164711886.1 pilus assembly protein PilM -

Sequence

Protein


Download         Length: 442 a.a.        Molecular weight: 47997.30 Da        Isoelectric Point: 8.8607

>NTDB_id=321226 EAE30_RS17175 WP_164711885.1 2587309..2588637(-) (pilQ) [Vibrio zhugei strain HBUAS61001]
MNNKLLGLFLIMGCCALFSAKSAFSEVMRTPAQREPSSSSLLSLQFQSIDVRRVLTLLAEKQGLNVVIDERVSGSISVNL
QQVSWQNALEVILRMKGLGYQTRHNVLWIAPLSVIKQQQGAVSSLTETMHYHRVPVHYAKATELLTLITAKNGQSMLSEQ
GSVQVDKRTNSLLVYDTDQHTKRIKQLVAIVDVPMKQVVIEARIVTMNQGELADIGVRWGAVQGPRSARISGTLEGLNGK
EGDSIANDLSVQIPSATPRAASLAFQVATLGSNTLLDLELSALQSESKAEIISSPRLLTTDEKPAYIEQGTEIPYLEAAS
SGATSVSFRKAVLSLKVTPHITPSNRLILDLNVTQDRPGSSVAIGTGDAVAIQTQRIGTQVMVNDGETIVLGGIYQHSLT
KGVEKVPLLGDLPLLGQLFRRSYEEKGKNELVIFVTPKVIIQ

Nucleotide


Download         Length: 1329 bp        

>NTDB_id=321226 EAE30_RS17175 WP_164711885.1 2587309..2588637(-) (pilQ) [Vibrio zhugei strain HBUAS61001]
ATGAACAATAAATTACTTGGCTTATTTTTGATAATGGGATGCTGTGCACTTTTTAGTGCTAAGAGTGCCTTCTCTGAGGT
AATGAGGACACCAGCCCAAAGAGAGCCGTCATCGTCATCATTGTTGTCGTTACAATTTCAGTCAATTGATGTGCGACGAG
TCCTAACACTATTGGCAGAAAAACAAGGTCTAAATGTGGTAATCGATGAACGAGTATCAGGTAGCATTTCTGTCAATTTA
CAGCAGGTTTCTTGGCAAAACGCACTGGAAGTCATTTTACGCATGAAAGGTCTAGGCTATCAGACGCGTCATAATGTGTT
ATGGATAGCGCCATTGAGCGTGATTAAGCAGCAACAAGGGGCAGTCAGTTCACTGACGGAAACAATGCATTACCATCGGG
TGCCGGTGCATTACGCGAAAGCCACCGAGCTTCTCACCTTGATCACCGCCAAGAATGGGCAGTCCATGCTCTCTGAGCAA
GGGAGTGTTCAAGTCGATAAGCGCACCAATTCTTTGTTGGTCTACGATACTGACCAACACACGAAACGTATTAAACAGCT
GGTTGCCATTGTGGATGTTCCCATGAAGCAAGTGGTGATAGAGGCACGCATTGTGACTATGAATCAAGGCGAACTCGCGG
ACATCGGGGTGCGTTGGGGGGCAGTTCAAGGCCCGCGCAGTGCACGGATATCCGGTACGCTCGAAGGGCTGAATGGGAAA
GAGGGCGACAGTATTGCAAATGACCTTAGTGTTCAAATCCCCAGTGCCACTCCCCGTGCGGCAAGCTTGGCCTTTCAGGT
GGCAACGCTGGGCTCTAATACGTTATTAGATTTGGAATTGTCAGCGTTACAGAGTGAGTCGAAAGCGGAAATTATTTCCA
GTCCTCGGTTACTCACCACCGATGAAAAACCGGCTTACATTGAGCAAGGGACCGAAATTCCTTATTTAGAAGCGGCGTCG
AGCGGAGCGACCTCAGTGTCATTCCGTAAAGCCGTGTTGAGCTTAAAAGTGACGCCACACATTACGCCAAGTAACCGGTT
AATTTTAGATTTGAATGTCACACAAGACCGCCCTGGTAGCTCCGTTGCGATTGGCACGGGAGACGCTGTAGCCATTCAAA
CTCAACGGATTGGTACTCAGGTCATGGTGAATGATGGAGAAACGATCGTTTTAGGTGGCATTTATCAACATAGCTTAACC
AAAGGGGTGGAAAAAGTCCCACTCTTGGGAGACTTGCCGCTCCTTGGGCAACTGTTTCGCCGCAGTTATGAAGAAAAAGG
CAAAAATGAATTAGTCATCTTTGTGACTCCAAAGGTTATTATTCAATAA

Domains

Predicted by InterproScan.

Bacterial type II/III secretion system short domain (Secretin_N)

(132-197)

Bacterial type II and III secretion system protein (Secretin)

(282-440)

Secretin and TonB N terminus short domain (STN)

(64-111)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Vibrio campbellii strain DS40M4

53.791

95.475

0.514
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

50.448

100

0.509
  pilQ Vibrio cholerae strain A1552

50.448

100

0.509
  pilQ Legionella pneumophila strain ERS1305867

41.57

97.964

0.407
  pilQ Legionella pneumophila strain Lp02

41.339

97.964

0.405
  comE Haemophilus influenzae 86-028NP

40.547

99.321

0.403
  comE Haemophilus influenzae Rd KW20

40.091

99.321

0.398
  pilQ Pseudomonas aeruginosa PAK

38.747

97.511

0.378
  comQ Acinetobacter baylyi ADP1

36.961

99.774

0.369

Multiple sequence alignment