Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   TMZ1T_RS05740 Genome accession   NC_011662
Coordinates   1301250..1302965 (-) Length   571 a.a.
NCBI ID   WP_004302458.1    Uniprot ID   N6YY93
Organism   Thauera aminoaromatica     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1296250..1307965
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  TMZ1T_RS05720 (Tmz1t_1171) - 1298019..1298645 (-) 627 WP_004302465.1 DUF502 domain-containing protein -
  TMZ1T_RS05725 (Tmz1t_1172) - 1298728..1298991 (-) 264 WP_012584903.1 FmdB family zinc ribbon protein -
  TMZ1T_RS05730 (Tmz1t_1173) pilD 1299149..1300000 (-) 852 WP_004302462.1 A24 family peptidase Machinery gene
  TMZ1T_RS05735 (Tmz1t_1174) pilC 1300005..1301234 (-) 1230 WP_004302460.1 type II secretion system F family protein Machinery gene
  TMZ1T_RS05740 (Tmz1t_1175) pilB 1301250..1302965 (-) 1716 WP_004302458.1 type IV-A pilus assembly ATPase PilB Machinery gene
  TMZ1T_RS05745 (Tmz1t_1176) - 1303054..1304334 (-) 1281 WP_004302456.1 HlyC/CorC family transporter -
  TMZ1T_RS05750 (Tmz1t_1177) - 1304418..1305248 (-) 831 WP_012584904.1 inner membrane protein YpjD -
  TMZ1T_RS05755 (Tmz1t_1178) ffh 1305297..1306667 (+) 1371 WP_004302452.1 signal recognition particle protein -
  TMZ1T_RS05760 (Tmz1t_1179) - 1306684..1307460 (+) 777 WP_004302451.1 EI24 domain-containing protein -

Sequence

Protein


Download         Length: 571 a.a.        Molecular weight: 61850.18 Da        Isoelectric Point: 5.4575

>NTDB_id=32009 TMZ1T_RS05740 WP_004302458.1 1301250..1302965(-) (pilB) [Thauera aminoaromatica]
MAATSKPALSGFARALIQHGHLKEADAIACTTHAGEAATAFILEVANRGLMSCAAMARFAAETFGYPLLDLAAFDAAMFA
REAVDRKLLAKHQVAPLAKRQSRITLATADPSNLRALDEIRFQTGLQIELVIVEADKLKRAVEALSESAADTLKELTGEA
FDMDLLQQDGPAQPRDEEEANEVDDAPVVKFIQKVLIDAINEGASDVHFEPYEKFYRIRVRTDGILRDIAQPPLVLKDKI
AARIKVISRLDISEKRVPQDGRMKLVLSRNKAIDFRVSTLPTLHGEKIVMRILDPSSAMLGIDALGYDPEQREALLAAVE
RPYGMILVTGPTGSGKTVSLYTCLNLLNKPGVNISTAEDPAEINLAGINQVNVNEKAGLTFSAALRAFLRQDPDVIMVGE
IRDIETAEIAIKAAQTGHLVMSTLHTNDAPSTLERLKNMGVAPFNIASSVILITAQRLARRLCSCKKPVDIPAQALLEAG
FEPDELDGGWQPMGPGGCERCKGSGYKGRVGIYQVMPISEEIARIIMTGGNSMDIAAQARSEGVLDLRQSGLRKVRQGIT
SLEEVLATTNE

Nucleotide


Download         Length: 1716 bp        

>NTDB_id=32009 TMZ1T_RS05740 WP_004302458.1 1301250..1302965(-) (pilB) [Thauera aminoaromatica]
ATGGCGGCGACCTCCAAACCGGCCCTGAGCGGCTTCGCCAGGGCCCTGATCCAGCATGGCCACCTCAAGGAGGCGGACGC
GATCGCATGCACCACCCATGCGGGCGAGGCCGCGACCGCCTTCATCCTCGAGGTCGCCAACCGCGGCCTGATGAGCTGCG
CGGCGATGGCCCGCTTCGCCGCCGAGACCTTCGGCTATCCGCTCCTCGACCTCGCCGCCTTCGATGCGGCGATGTTCGCG
CGCGAGGCGGTCGACCGCAAGCTGCTCGCCAAGCACCAGGTCGCGCCGCTCGCCAAGCGCCAGAGCCGCATCACGCTCGC
CACCGCCGACCCTTCCAACCTGCGCGCGCTGGACGAGATCCGCTTCCAGACCGGCCTGCAGATCGAGCTGGTGATCGTCG
AGGCGGACAAGCTCAAGCGCGCGGTCGAGGCCCTGTCCGAGTCGGCCGCAGACACGCTCAAGGAGCTCACCGGCGAAGCC
TTCGACATGGACCTGCTGCAGCAGGACGGTCCCGCGCAGCCGCGCGACGAGGAGGAAGCCAACGAGGTCGATGACGCCCC
GGTCGTCAAGTTCATCCAGAAGGTGCTGATCGACGCGATCAACGAGGGCGCCTCGGACGTCCACTTCGAGCCCTACGAGA
AGTTCTACCGCATCCGCGTGCGCACCGACGGCATCCTGCGCGACATCGCCCAGCCGCCGCTGGTGCTCAAGGACAAGATC
GCCGCGCGCATCAAGGTGATCTCGCGCCTCGACATCTCGGAGAAGCGCGTGCCGCAGGACGGGCGCATGAAGCTCGTGTT
GTCGCGCAACAAGGCGATCGACTTCCGCGTCTCGACGCTGCCGACCCTGCACGGCGAGAAGATCGTGATGCGCATCCTCG
ATCCGAGCTCGGCGATGCTCGGCATCGACGCGCTCGGCTACGACCCCGAGCAGCGCGAGGCGCTGCTCGCCGCGGTCGAG
CGCCCCTACGGCATGATCCTGGTCACCGGCCCCACCGGCTCGGGCAAGACGGTGTCGCTGTACACCTGCCTCAACCTGCT
CAACAAGCCGGGCGTGAACATCTCCACCGCCGAGGACCCTGCCGAGATCAATCTCGCCGGCATCAACCAGGTCAACGTCA
ACGAGAAGGCCGGCCTCACCTTCTCCGCCGCGCTGCGCGCCTTCCTGCGCCAGGACCCGGACGTGATCATGGTGGGCGAG
ATCCGCGACATCGAGACCGCCGAGATCGCCATCAAGGCCGCGCAGACCGGCCACCTGGTGATGTCCACCCTGCACACCAA
CGACGCGCCCTCCACGCTCGAGCGCCTCAAGAACATGGGCGTGGCGCCGTTCAATATCGCATCCTCGGTGATCCTGATCA
CGGCCCAGCGCCTCGCCCGCCGGCTATGCTCCTGCAAGAAGCCCGTCGACATTCCCGCCCAGGCCCTGCTGGAGGCCGGC
TTCGAGCCCGACGAGCTCGACGGCGGCTGGCAGCCGATGGGACCGGGCGGTTGCGAACGCTGCAAGGGCAGCGGCTACAA
GGGCCGGGTGGGCATCTACCAGGTCATGCCGATCAGCGAGGAGATCGCCCGCATCATCATGACCGGCGGAAACTCCATGG
ACATCGCCGCCCAGGCCCGCAGCGAGGGCGTGCTCGACCTGCGCCAGTCGGGCCTGCGCAAGGTGCGCCAAGGCATCACC
TCGCTCGAAGAAGTGCTGGCGACGACCAACGAATAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(48-149)

Type II/IV secretion system protein (T2SSE)

(194-461)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB N6YY93

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

55.826

100

0.562
  pilB Acinetobacter baylyi ADP1

55.672

100

0.559
  pilB Legionella pneumophila strain ERS1305867

52.381

99.299

0.52
  pilF Neisseria gonorrhoeae MS11

51.59

99.124

0.511
  pilB Vibrio cholerae strain A1552

52.353

89.317

0.468
  pilB Vibrio parahaemolyticus RIMD 2210633

50

91.419

0.457
  pilB Vibrio campbellii strain DS40M4

48.952

91.944

0.45
  pilF Thermus thermophilus HB27

40.678

92.995

0.378
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.509

92.644

0.366

Multiple sequence alignment