Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   TMZ1T_RS05730 Genome accession   NC_011662
Coordinates   1299149..1300000 (-) Length   283 a.a.
NCBI ID   WP_004302462.1    Uniprot ID   N6Y6B0
Organism   Thauera aminoaromatica     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1294149..1305000
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  TMZ1T_RS05710 (Tmz1t_1169) - 1294337..1295986 (-) 1650 WP_004302469.1 tetratricopeptide repeat-containing response regulator -
  TMZ1T_RS05715 (Tmz1t_1170) aspS 1296132..1297931 (-) 1800 WP_004302467.1 aspartate--tRNA ligase -
  TMZ1T_RS05720 (Tmz1t_1171) - 1298019..1298645 (-) 627 WP_004302465.1 DUF502 domain-containing protein -
  TMZ1T_RS05725 (Tmz1t_1172) - 1298728..1298991 (-) 264 WP_012584903.1 FmdB family zinc ribbon protein -
  TMZ1T_RS05730 (Tmz1t_1173) pilD 1299149..1300000 (-) 852 WP_004302462.1 A24 family peptidase Machinery gene
  TMZ1T_RS05735 (Tmz1t_1174) pilC 1300005..1301234 (-) 1230 WP_004302460.1 type II secretion system F family protein Machinery gene
  TMZ1T_RS05740 (Tmz1t_1175) pilB 1301250..1302965 (-) 1716 WP_004302458.1 type IV-A pilus assembly ATPase PilB Machinery gene
  TMZ1T_RS05745 (Tmz1t_1176) - 1303054..1304334 (-) 1281 WP_004302456.1 HlyC/CorC family transporter -

Sequence

Protein


Download         Length: 283 a.a.        Molecular weight: 30880.61 Da        Isoelectric Point: 7.4135

>NTDB_id=32007 TMZ1T_RS05730 WP_004302462.1 1299149..1300000(-) (pilD) [Thauera aminoaromatica]
MIELLRDPLAFTILSGLVGLFVGSFLNVVIHRLPRMMERDWHAQAAELRGEQAPANERFNLATPRSRCPHCGHQITMLEN
IPIVSYVVLRGRCAHCGAGISRRYPIVEALSALLSAYAAWHFGFGASALGALLFVWAMVALTFIDLDTQLLPDDITLPLL
WLGLAFNLGTTFAELPDAVIGAMAGYLALWAVFWLFKLATGKEGMGYGDFKLLAAIGAWLGWQMLPLTILLSSLVGAVVG
ILLIVVARHGRNVPIPFGPYLAAAGLIALFWGERITERYLGLL

Nucleotide


Download         Length: 852 bp        

>NTDB_id=32007 TMZ1T_RS05730 WP_004302462.1 1299149..1300000(-) (pilD) [Thauera aminoaromatica]
ATGATCGAGCTTCTCCGCGACCCGCTCGCCTTCACCATCCTCTCCGGCCTGGTCGGACTCTTCGTCGGCAGCTTCCTCAA
CGTCGTGATCCATCGGCTGCCCAGGATGATGGAGCGCGACTGGCACGCCCAGGCGGCCGAGCTGCGTGGCGAGCAAGCAC
CCGCCAACGAGCGCTTCAACCTCGCCACGCCGCGCTCGCGCTGCCCGCACTGCGGGCACCAGATCACGATGCTGGAGAAC
ATCCCGATCGTCAGCTACGTGGTGCTGCGCGGGCGCTGCGCACATTGCGGCGCCGGCATCAGCAGGCGCTACCCGATCGT
CGAAGCCCTCTCCGCGCTGCTGTCGGCCTACGCCGCCTGGCATTTCGGCTTCGGCGCCAGCGCGCTCGGGGCCCTGCTGT
TCGTGTGGGCGATGGTCGCGCTCACCTTCATCGACCTCGACACCCAGCTGCTGCCCGACGACATCACCCTGCCGCTGCTG
TGGCTGGGCCTCGCCTTCAACCTCGGCACCACCTTCGCCGAGCTTCCCGACGCGGTGATCGGCGCGATGGCGGGCTACCT
CGCGCTGTGGGCGGTGTTCTGGCTGTTCAAGCTCGCCACCGGCAAGGAGGGCATGGGCTACGGAGACTTCAAGCTGCTCG
CCGCGATCGGCGCCTGGCTGGGCTGGCAGATGCTGCCGCTGACCATCCTGCTGTCCTCGCTGGTGGGCGCGGTGGTCGGC
ATCCTGCTGATCGTCGTCGCCCGCCACGGCCGCAACGTGCCGATCCCCTTCGGCCCCTACCTCGCCGCGGCCGGCCTGAT
CGCGCTGTTCTGGGGCGAGCGCATCACCGAGCGCTACCTCGGGCTGCTCTGA

Domains

Predicted by InterproScan.

Bacterial Peptidase A24 N-terminal domain (A24_N_bact)

(17-122)

Type IV leader peptidase family (Peptidase_A24)

(133-241)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB N6Y6B0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Vibrio cholerae strain A1552

54.71

97.527

0.534
  pilD Vibrio campbellii strain DS40M4

52.174

97.527

0.509
  pilD Acinetobacter baumannii D1279779

49.104

98.587

0.484
  pilD Acinetobacter nosocomialis M2

49.104

98.587

0.484
  pilD Neisseria gonorrhoeae MS11

50.391

90.459

0.456

Multiple sequence alignment