Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   D7032_RS02335 Genome accession   NZ_CP032664
Coordinates   500659..502365 (-) Length   568 a.a.
NCBI ID   WP_208192726.1    Uniprot ID   A0A7T8E9D4
Organism   Shewanella algae strain HIDE     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 495659..507365
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  D7032_RS02300 (D7032_02295) - 495896..496162 (-) 267 Protein_428 transposase -
  D7032_RS02305 (D7032_02300) mutT 496240..496638 (-) 399 WP_025012058.1 8-oxo-dGTP diphosphatase MutT -
  D7032_RS02310 (D7032_02305) yacG 496638..496850 (-) 213 WP_025886789.1 DNA gyrase inhibitor YacG -
  D7032_RS02315 (D7032_02310) zapD 496895..497635 (-) 741 WP_028779562.1 cell division protein ZapD -
  D7032_RS02320 (D7032_02315) coaE 497632..498243 (-) 612 WP_054744929.1 dephospho-CoA kinase -
  D7032_RS02325 (D7032_02320) pilD 498244..499158 (-) 915 WP_208192724.1 A24 family peptidase Machinery gene
  D7032_RS02330 (D7032_02325) pilC 499234..500502 (-) 1269 WP_208192725.1 type II secretion system F family protein Machinery gene
  D7032_RS02335 (D7032_02330) pilB 500659..502365 (-) 1707 WP_208192726.1 type IV-A pilus assembly ATPase PilB Machinery gene
  D7032_RS02340 (D7032_02335) - 502431..502847 (-) 417 WP_208192727.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  D7032_RS02345 (D7032_02340) nadC 503715..504575 (-) 861 WP_025012052.1 carboxylating nicotinate-nucleotide diphosphorylase -
  D7032_RS02350 (D7032_02345) ampD 504734..505312 (+) 579 WP_186265262.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  D7032_RS02355 (D7032_02350) ampE 505366..506217 (+) 852 WP_345861628.1 beta-lactamase regulator AmpE -
  D7032_RS02360 (D7032_02355) pdhR 506492..507244 (+) 753 WP_025886793.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -

Sequence

Protein


Download         Length: 568 a.a.        Molecular weight: 63028.13 Da        Isoelectric Point: 5.1753

>NTDB_id=317704 D7032_RS02335 WP_208192726.1 500659..502365(-) (pilB) [Shewanella algae strain HIDE]
MPTTGLHLGLSTLFVRKGLLDESSLSDAIKQSQKNKQALVTTLVTSQLLSARTIAELCYEEYGTPLLDLNEFDLSSIPEE
FLNKKLIEKHRCLPLFKRGNRLYIATSDPTNIAALEDFQFSAGLHAEAILVEEDKLSKALEKVLEEDLSSLELDGLDEES
LAGIEVTDTDKRQQEENEGKDDAPIVIYINKILTDAIRKGASDLHFEPYEKRYRIRFRIDGILHEVSEPPISLSGRISAR
LKVMSKLDIAERRVPQDGRIKMRLSRNKSIDFRVSTLPTLWGEKIVMRILDSSSAQLGIEKLGYEEDQKQAYLEMLARPQ
GMILVTGPTGSGKTVSLYTGLNILNTEERNISTAEDPVEINLEGVNQVHINPKAGLTFASALRSFLRQDPDVVMVGEIRD
LETAEIAIKAAQTGHLVLSTLHTNSSAETLTRLLNMGVPGYNIASSVNLIIAQRLARRLCPECRQPEEIPEHELLQLGFS
QQQIDAGFTPYKPCGCDLCSGGYKGRVGIYEVMKMSDEIARTIMEGGNSLQIATTAKQQGMRDLRLSGLLKVIQGVTSIA
EVNRVTSY

Nucleotide


Download         Length: 1707 bp        

>NTDB_id=317704 D7032_RS02335 WP_208192726.1 500659..502365(-) (pilB) [Shewanella algae strain HIDE]
ATGCCAACAACAGGACTTCACTTAGGACTTTCGACTCTGTTTGTCAGAAAAGGGCTACTCGATGAGAGTAGCCTGTCGGA
TGCCATTAAACAGTCGCAAAAGAACAAACAAGCTCTGGTCACCACTCTGGTGACCAGTCAACTTTTATCAGCCCGTACCA
TCGCCGAACTCTGCTACGAAGAGTATGGCACCCCACTGCTGGATCTCAATGAGTTTGATTTAAGCAGTATTCCGGAAGAG
TTTCTCAACAAAAAACTTATCGAAAAACATAGGTGCCTGCCGTTATTTAAGCGTGGCAACCGCCTCTATATCGCCACCTC
AGATCCCACCAATATCGCTGCACTTGAGGATTTTCAGTTCAGCGCCGGCCTGCACGCCGAAGCAATACTGGTAGAGGAGG
ATAAGCTCAGTAAAGCGCTGGAAAAAGTGCTTGAAGAAGACCTGAGTTCGCTGGAACTGGATGGTCTCGATGAGGAGTCC
CTCGCAGGTATCGAGGTCACCGACACCGACAAACGTCAACAGGAAGAGAATGAAGGCAAGGATGACGCCCCCATAGTCAT
CTATATCAATAAGATTTTGACCGATGCCATCCGCAAGGGCGCATCAGATCTGCATTTCGAGCCCTACGAGAAGCGCTACC
GCATTCGCTTTCGTATCGATGGCATCCTCCACGAGGTGTCCGAACCACCGATTTCTCTCTCGGGCCGTATATCGGCCCGC
TTGAAAGTAATGTCCAAATTGGATATCGCCGAGCGCCGGGTACCTCAGGATGGCCGCATCAAGATGAGGCTGTCGCGTAA
CAAGAGTATCGATTTCCGGGTAAGTACCCTGCCCACCCTTTGGGGTGAAAAGATAGTGATGCGGATCCTCGACTCCTCTT
CGGCGCAATTAGGCATCGAAAAACTCGGCTATGAGGAAGACCAAAAACAAGCCTATCTGGAAATGCTGGCAAGACCCCAG
GGGATGATCTTGGTAACAGGCCCTACCGGCTCAGGGAAAACCGTATCCCTTTATACTGGCCTCAATATCCTCAATACCGA
AGAGCGCAATATCAGCACCGCCGAAGACCCGGTAGAGATCAATCTGGAAGGGGTCAACCAGGTACATATCAACCCCAAGG
CAGGGTTAACCTTCGCCTCGGCGCTGCGCTCCTTTTTGCGTCAGGACCCGGATGTGGTGATGGTGGGGGAAATCCGCGAC
CTGGAAACCGCCGAAATCGCCATCAAGGCGGCGCAAACCGGCCACTTGGTACTCTCAACCCTGCATACCAACTCATCGGC
CGAGACTCTCACCCGCCTGCTCAACATGGGCGTCCCAGGTTACAACATTGCCAGTTCAGTCAATCTGATTATTGCCCAGC
GTCTGGCCCGCCGCCTCTGCCCCGAATGCCGCCAACCGGAAGAGATCCCCGAGCATGAACTGCTGCAGCTGGGATTTAGT
CAGCAGCAGATAGACGCCGGCTTTACCCCATACAAGCCCTGCGGCTGCGACCTCTGCTCCGGTGGTTATAAGGGAAGGGT
CGGTATCTATGAGGTAATGAAGATGTCCGATGAAATCGCCAGAACCATTATGGAGGGCGGTAATTCGCTGCAGATTGCCA
CTACTGCCAAACAGCAGGGGATGCGCGACCTGCGCCTTTCTGGCCTTTTAAAAGTGATTCAAGGCGTCACCAGTATCGCC
GAAGTGAACCGGGTCACCAGCTACTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(191-458)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(43-145)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A7T8E9D4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

58.541

98.944

0.579
  pilB Acinetobacter baumannii D1279779

58.111

98.768

0.574
  pilB Legionella pneumophila strain ERS1305867

56.184

99.648

0.56
  pilB Vibrio parahaemolyticus RIMD 2210633

53.345

100

0.533
  pilB Vibrio cholerae strain A1552

53.455

96.831

0.518
  pilB Vibrio campbellii strain DS40M4

51.865

99.12

0.514
  pilF Neisseria gonorrhoeae MS11

47.965

99.472

0.477
  pilF Thermus thermophilus HB27

38.23

99.472

0.38
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

37.857

98.592

0.373

Multiple sequence alignment