Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   D7032_RS02325 Genome accession   NZ_CP032664
Coordinates   498244..499158 (-) Length   304 a.a.
NCBI ID   WP_208192724.1    Uniprot ID   -
Organism   Shewanella algae strain HIDE     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 493244..504158
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  D7032_RS02300 (D7032_02295) - 495896..496162 (-) 267 Protein_428 transposase -
  D7032_RS02305 (D7032_02300) mutT 496240..496638 (-) 399 WP_025012058.1 8-oxo-dGTP diphosphatase MutT -
  D7032_RS02310 (D7032_02305) yacG 496638..496850 (-) 213 WP_025886789.1 DNA gyrase inhibitor YacG -
  D7032_RS02315 (D7032_02310) zapD 496895..497635 (-) 741 WP_028779562.1 cell division protein ZapD -
  D7032_RS02320 (D7032_02315) coaE 497632..498243 (-) 612 WP_054744929.1 dephospho-CoA kinase -
  D7032_RS02325 (D7032_02320) pilD 498244..499158 (-) 915 WP_208192724.1 A24 family peptidase Machinery gene
  D7032_RS02330 (D7032_02325) pilC 499234..500502 (-) 1269 WP_208192725.1 type II secretion system F family protein Machinery gene
  D7032_RS02335 (D7032_02330) pilB 500659..502365 (-) 1707 WP_208192726.1 type IV-A pilus assembly ATPase PilB Machinery gene
  D7032_RS02340 (D7032_02335) - 502431..502847 (-) 417 WP_208192727.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -

Sequence

Protein


Download         Length: 304 a.a.        Molecular weight: 33873.23 Da        Isoelectric Point: 7.2147

>NTDB_id=317702 D7032_RS02325 WP_208192724.1 498244..499158(-) (pilD) [Shewanella algae strain HIDE]
MSEFIAVLSHNQWLFLVLCFLFASVIGSFLNVVIHRLPVMMKREWQQECNQYLQEYQPEFAKQLGEAKLNAPIDNYPAKY
NLILPKSACPKCGAHIKPWHNLPIIGYLLLGGKCASCNTAISARYPIFELLTALLISTLGWYFGPTLQFVYATILTFGLI
ALSGIDLDEMLLPDQITLPLLWLGLLVNISGTFTNLTDATIGAAAGYLSLWSVYWLFKLLTGKEGMGYGDFKLMALFGAW
LGWQMLPLIILFSSLVGALVGVLLILFKNLNRGNPIPFGPYIAIAGWVALVWGQQIVDWYLGTL

Nucleotide


Download         Length: 915 bp        

>NTDB_id=317702 D7032_RS02325 WP_208192724.1 498244..499158(-) (pilD) [Shewanella algae strain HIDE]
ATGTCAGAATTTATTGCCGTATTGAGCCACAACCAGTGGCTCTTTCTTGTTCTCTGCTTTTTGTTTGCTTCTGTAATAGG
CAGCTTTCTCAATGTGGTGATCCACCGCCTGCCGGTAATGATGAAGCGCGAATGGCAGCAGGAATGCAATCAATACCTGC
AGGAATATCAACCCGAATTCGCCAAACAGCTGGGTGAAGCCAAGCTTAATGCACCAATAGATAACTATCCGGCCAAATAC
AATCTGATACTGCCGAAGTCTGCCTGCCCCAAGTGTGGCGCCCATATCAAACCCTGGCACAACCTCCCCATAATCGGCTA
TTTGTTGCTTGGCGGTAAATGCGCCTCCTGCAACACAGCTATTTCAGCGAGATACCCGATATTTGAGCTGCTGACCGCCC
TGCTTATCAGTACTCTCGGGTGGTATTTTGGCCCGACACTGCAGTTTGTTTACGCCACTATTCTCACCTTTGGATTGATA
GCCCTCAGCGGGATAGATTTGGATGAAATGCTGCTGCCGGATCAGATTACCCTGCCGCTACTGTGGCTTGGTTTACTGGT
TAATATCTCGGGCACCTTTACTAATCTGACCGATGCCACCATAGGGGCCGCTGCCGGCTATTTAAGCCTCTGGTCGGTCT
ACTGGCTATTTAAACTGCTCACCGGCAAGGAAGGCATGGGCTATGGCGATTTCAAGCTGATGGCATTGTTTGGCGCCTGG
CTTGGCTGGCAGATGTTACCTCTAATTATTCTGTTCTCTTCTCTGGTCGGTGCCCTAGTAGGCGTGTTACTTATTCTGTT
CAAGAACCTTAATCGGGGTAACCCTATCCCCTTTGGCCCTTATATTGCTATCGCAGGTTGGGTCGCTTTGGTGTGGGGGC
AACAGATTGTCGACTGGTATTTGGGAACACTCTAG

Domains

Predicted by InterproScan.

Bacterial Peptidase A24 N-terminal domain (A24_N_bact)

(21-143)

Type IV leader peptidase family (Peptidase_A24)

(154-262)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Vibrio campbellii strain DS40M4

48.837

99.013

0.484
  pilD Vibrio cholerae strain A1552

49.658

96.053

0.477
  pilD Acinetobacter nosocomialis M2

47.682

99.342

0.474
  pilD Acinetobacter baumannii D1279779

47.351

99.342

0.47
  pilD Neisseria gonorrhoeae MS11

45.196

92.434

0.418

Multiple sequence alignment