Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   E4311_RS04430 Genome accession   NZ_CP039403
Coordinates   924109..925494 (-) Length   461 a.a.
NCBI ID   WP_024231534.1    Uniprot ID   -
Organism   Escherichia coli strain 382634_2f     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 919109..930494
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  E4311_RS04400 (E4311_04390) mutT 919558..919956 (+) 399 WP_000736031.1 8-oxo-dGTP diphosphatase MutT -
  E4311_RS04405 (E4311_04395) yacG 920044..920241 (-) 198 WP_000005042.1 DNA gyrase inhibitor YacG -
  E4311_RS04410 (E4311_04400) zapD 920251..920994 (-) 744 WP_001194731.1 cell division protein ZapD -
  E4311_RS04415 (E4311_04405) coaE 920994..921614 (-) 621 WP_001269524.1 dephospho-CoA kinase -
  E4311_RS23295 - 921639..921683 (+) 45 WP_120795372.1 protein YacM -
  E4311_RS04420 (E4311_04410) guaC 921839..922882 (+) 1044 WP_001217338.1 GMP reductase -
  E4311_RS04425 (E4311_04415) hofC 922917..924119 (-) 1203 WP_000157242.1 protein transport protein HofC -
  E4311_RS04430 (E4311_04420) pilB 924109..925494 (-) 1386 WP_024231534.1 type II secretion system protein GspE Machinery gene
  E4311_RS04435 (E4311_04425) pilA 925504..925944 (-) 441 WP_000360914.1 prepilin peptidase-dependent pilin Machinery gene
  E4311_RS04440 (E4311_04430) nadC 926148..927041 (-) 894 WP_001135168.1 carboxylating nicotinate-nucleotide diphosphorylase -
  E4311_RS04445 (E4311_04435) ampD 927129..927680 (+) 552 WP_000923703.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  E4311_RS04450 (E4311_04440) ampE 927677..928531 (+) 855 WP_000171984.1 beta-lactamase regulator AmpE -
  E4311_RS04455 (E4311_04445) aroP 928574..929947 (-) 1374 WP_072172188.1 aromatic amino acid transporter AroP -

Sequence

Protein


Download         Length: 461 a.a.        Molecular weight: 50662.31 Da        Isoelectric Point: 6.5396

>NTDB_id=317540 E4311_RS04430 WP_024231534.1 924109..925494(-) (pilB) [Escherichia coli strain 382634_2f]
MNIPQLTALCLRYQGVLLDASEEVVHVAVVDAPSHELLDALHFATTKRIEITCWTRQQMEGHASRTQQTLPVAVQEKHQP
KAELLTRTLQSALEQRASDIHIEPADNAYRIRLRIDGVLHPLPDVSPDAGVALTARLKVLGNLDIAEHRLPQDGQFTVEL
AGNAVSFRIATLACRGGEKVVLRLLQQVNQALDVNTLGMQPSQLVDFAHALQQPQGLVLVTGPTGSGKTVTLYSALQMLN
TADINICSVEDPVEIPIAGLNQTQIHSRAGLTFQGVLRALLRQDPDVIMIGEIRDGETAEIAIKAAQTGHLVLSTLHTNS
TCETLVRLQQMGVARWMLSSALTLVIAQRLVRKLCPHCRRQQGEPIHIPDNVWPSPLPHWQAPGCVHCYHGFYGRTALFE
VLPITPVIRQLISANTDVESLETHARQTGMRTLFENGCLAVEQGLTTFEELIRVLGMPHGE

Nucleotide


Download         Length: 1386 bp        

>NTDB_id=317540 E4311_RS04430 WP_024231534.1 924109..925494(-) (pilB) [Escherichia coli strain 382634_2f]
ATGAATATTCCACAGCTCACTGCCCTGTGTCTGCGTTATCAGGGAGTCTTGCTGGATGCCAGCGAAGAGGTGGTTCATGT
TGCGGTAGTCGATGCACCTTCGCATGAGCTACTGGACGCATTGCATTTCGCTACCACCAAACGTATTGAGATCACCTGCT
GGACGCGCCAACAAATGGAAGGTCACGCCAGTCGCACACAACAGACATTGCCCGTAGCTGTTCAGGAGAAGCATCAGCCC
AAAGCAGAGTTGCTGACTCGAACGTTACAATCTGCGCTGGAACAACGCGCGTCTGATATTCATATCGAACCAGCGGACAA
TGCCTACCGCATCCGCTTGCGTATCGACGGCGTATTGCATCCTTTACCGGACGTTTCACCGGATGCCGGAGTCGCATTAA
CCGCCAGATTAAAAGTGCTGGGAAACCTGGATATTGCGGAACATCGCCTGCCGCAGGACGGGCAATTCACTGTCGAACTG
GCAGGAAACGCCGTCTCATTTCGTATTGCGACCTTAGCATGTCGGGGTGGTGAAAAGGTGGTATTAAGGTTGTTACAGCA
GGTGAACCAGGCACTGGATGTTAACACGCTTGGAATGCAGCCGTCACAACTGGTGGACTTTGCTCATGCCTTGCAACAAC
CACAGGGACTGGTGCTGGTAACTGGCCCTACTGGCAGCGGCAAAACGGTCACGCTTTATAGTGCCCTGCAAATGCTGAAT
ACCGCTGACATTAATATTTGTAGCGTCGAAGATCCGGTTGAGATCCCCATAGCCGGACTAAACCAGACGCAAATCCATTC
GCGTGCCGGACTCACCTTTCAGGGCGTTTTGCGTGCGTTATTGCGCCAGGATCCTGACGTCATCATGATCGGAGAGATCC
GCGATGGCGAAACGGCAGAAATTGCCATTAAAGCCGCGCAAACCGGTCACCTGGTGTTGTCTACCCTACACACTAATTCC
ACCTGCGAAACGCTGGTACGTTTACAGCAAATGGGAGTCGCCCGCTGGATGCTATCATCGGCGCTTACGCTGGTAATAGC
CCAGCGTCTGGTACGCAAACTTTGCCCGCATTGTCGCCGGCAGCAAGGGGAGCCCATCCATATTCCAGACAATGTATGGC
CATCGCCGCTGCCCCACTGGCAGGCACCCGGTTGTGTACATTGCTACCACGGTTTTTATGGTCGCACGGCCTTATTTGAA
GTTCTGCCCATAACACCGGTCATACGTCAGCTTATTTCCGCTAATACCGACGTTGAATCGCTGGAAACGCACGCCCGACA
GACGGGTATGCGAACGCTTTTTGAAAACGGCTGCCTGGCCGTGGAGCAAGGCTTAACCACCTTTGAAGAGTTAATCCGCG
TATTGGGGATGCCGCATGGCGAGTAA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(88-353)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(8-68)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Glaesserella parasuis strain SC1401

41.037

100

0.412
  pilB Legionella pneumophila strain ERS1305867

49.479

83.297

0.412
  pilB Acinetobacter baylyi ADP1

39.53

100

0.401
  pilB Vibrio campbellii strain DS40M4

47.927

83.731

0.401
  pilB Vibrio cholerae strain A1552

46.154

84.599

0.39
  pilB Vibrio parahaemolyticus RIMD 2210633

45.641

84.599

0.386
  pilB Haemophilus influenzae 86-028NP

44.703

83.948

0.375
  pilF Neisseria gonorrhoeae MS11

44.156

83.514

0.369
  pilB Acinetobacter baumannii D1279779

42.893

85.466

0.367
  pilB Haemophilus influenzae Rd KW20

43.864

83.08

0.364