Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   E4311_RS04435 Genome accession   NZ_CP039403
Coordinates   925504..925944 (-) Length   146 a.a.
NCBI ID   WP_000360914.1    Uniprot ID   A0A0H2YVZ3
Organism   Escherichia coli strain 382634_2f     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 920504..930944
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  E4311_RS04415 (E4311_04405) coaE 920994..921614 (-) 621 WP_001269524.1 dephospho-CoA kinase -
  E4311_RS23295 - 921639..921683 (+) 45 WP_120795372.1 protein YacM -
  E4311_RS04420 (E4311_04410) guaC 921839..922882 (+) 1044 WP_001217338.1 GMP reductase -
  E4311_RS04425 (E4311_04415) hofC 922917..924119 (-) 1203 WP_000157242.1 protein transport protein HofC -
  E4311_RS04430 (E4311_04420) pilB 924109..925494 (-) 1386 WP_024231534.1 type II secretion system protein GspE Machinery gene
  E4311_RS04435 (E4311_04425) pilA 925504..925944 (-) 441 WP_000360914.1 prepilin peptidase-dependent pilin Machinery gene
  E4311_RS04440 (E4311_04430) nadC 926148..927041 (-) 894 WP_001135168.1 carboxylating nicotinate-nucleotide diphosphorylase -
  E4311_RS04445 (E4311_04435) ampD 927129..927680 (+) 552 WP_000923703.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  E4311_RS04450 (E4311_04440) ampE 927677..928531 (+) 855 WP_000171984.1 beta-lactamase regulator AmpE -
  E4311_RS04455 (E4311_04445) aroP 928574..929947 (-) 1374 WP_072172188.1 aromatic amino acid transporter AroP -

Sequence

Protein


Download         Length: 146 a.a.        Molecular weight: 15620.77 Da        Isoelectric Point: 4.5237

>NTDB_id=317541 E4311_RS04435 WP_000360914.1 925504..925944(-) (pilA) [Escherichia coli strain 382634_2f]
MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQTFVPYRTAVELCALEHGGLNTCDGGSNGIPSPTTTR
YVSAMSVAKGVVSLTGQESLNGLSVVMTPGWDNANGVTGWTRNCNIQSDSALQQACEDVFRFDDAN

Nucleotide


Download         Length: 441 bp        

>NTDB_id=317541 E4311_RS04435 WP_000360914.1 925504..925944(-) (pilA) [Escherichia coli strain 382634_2f]
ATGGACAAGCAACGCGGTTTTACACTTATCGAACTGATGGTGGTTATTGGCATCATTGCCATTTTAAGCGCCATTGGTAT
TCCCGCTTATCAAAACTACCTGCGCAAAGCCGCACTCACCGACATGCTACAAACCTTTGTGCCTTACCGTACCGCCGTAG
AGTTGTGCGCGCTGGAACATGGTGGATTAAATACCTGCGACGGTGGCAGCAATGGCATTCCCTCGCCTACCACCACCCGC
TATGTTTCAGCCATGAGTGTGGCAAAGGGCGTGGTGTCGCTGACCGGGCAAGAAAGTCTCAATGGACTAAGCGTCGTCAT
GACGCCGGGTTGGGATAACGCAAACGGCGTCACCGGCTGGACGCGTAACTGCAATATTCAAAGTGACAGCGCATTACAGC
AAGCCTGCGAAGATGTCTTCCGCTTTGATGACGCCAACTAA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-25)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0H2YVZ3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

43.089

84.247

0.363

Multiple sequence alignment