Detailed information    

insolico Bioinformatically predicted

Overview

Name   dprA/cilB/dalA   Type   Machinery gene
Locus tag   D7D53_RS03585 Genome accession   NZ_CP032621
Coordinates   723628..724476 (+) Length   282 a.a.
NCBI ID   WP_120770152.1    Uniprot ID   A0A387B2Z0
Organism   Streptococcus gwangjuensis strain KCOM 1679 (=ChDC B345)     
Function   ssDNA binding; loading RecA onto ssDNA; competence shut-off (predicted from homology)   
DNA processing Competence regulation

Genomic Context

Location: 718628..729476
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  D7D53_RS03555 (D7D53_03555) tatA 718845..719015 (+) 171 WP_061864669.1 twin-arginine translocase TatA/TatE family subunit -
  D7D53_RS03560 (D7D53_03560) - 719351..720058 (+) 708 WP_120770148.1 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase -
  D7D53_RS03565 (D7D53_03565) - 720063..721085 (+) 1023 WP_120770149.1 ribitol-5-phosphate dehydrogenase -
  D7D53_RS03570 (D7D53_03570) - 721102..721971 (+) 870 WP_120770150.1 phosphotransferase family protein -
  D7D53_RS03575 (D7D53_03575) - 721961..722839 (+) 879 WP_120770151.1 DMT family transporter -
  D7D53_RS03580 (D7D53_03580) - 722851..723540 (+) 690 WP_004269681.1 CTP--phosphocholine cytidylyltransferase -
  D7D53_RS03585 (D7D53_03585) dprA/cilB/dalA 723628..724476 (+) 849 WP_120770152.1 DNA-processing protein DprA Machinery gene
  D7D53_RS03590 (D7D53_03590) topA 724708..726795 (+) 2088 WP_120770153.1 type I DNA topoisomerase -
  D7D53_RS03595 (D7D53_03595) - 726901..727260 (+) 360 WP_120770154.1 YbaN family protein -
  D7D53_RS03600 (D7D53_03600) - 727353..727985 (+) 633 WP_120770155.1 copper homeostasis protein CutC -
  D7D53_RS03605 (D7D53_03605) - 728029..728685 (+) 657 WP_120770156.1 MmcQ/YjbR family DNA-binding protein -

Sequence

Protein


Download         Length: 282 a.a.        Molecular weight: 31103.82 Da        Isoelectric Point: 5.6756

>NTDB_id=317186 D7D53_RS03585 WP_120770152.1 723628..724476(+) (dprA/cilB/dalA) [Streptococcus gwangjuensis strain KCOM 1679 (=ChDC B345)]
MKITNYEIYKLKKSGLTNQQILKVLEYGENVDQELLLGDIADISGCRNPAVFMERYFQIDDAHLEKEFQKFPSFSILDDC
YPWDLSEIYDAPVLLFYKGNLDLLKFPKVAVVGSRACSKQGAKSVEKVIQGLENELVIVSGLAKGIDTAAHMAALQNGGK
TIAVIGTGLDVFYPKANKRLQNYIGNDHLVLSEYGPGEQPLKFHFPARNRIIAGLCRGVIVAEAKMRSGSLITCERAMEE
GRDVFAIPGSILDGLSDGCHHLIQEGAKLVTSGKDVLAEFEF

Nucleotide


Download         Length: 849 bp        

>NTDB_id=317186 D7D53_RS03585 WP_120770152.1 723628..724476(+) (dprA/cilB/dalA) [Streptococcus gwangjuensis strain KCOM 1679 (=ChDC B345)]
ATGAAAATAACAAATTATGAAATCTATAAGTTAAAAAAATCAGGTTTGACCAATCAACAGATTTTGAAAGTCCTAGAATA
CGGTGAAAATGTCGATCAGGAGCTTTTGTTGGGTGATATTGCAGATATATCAGGTTGCCGAAATCCAGCTGTTTTTATGG
AACGTTATTTTCAGATAGACGATGCGCATTTGGAAAAGGAGTTTCAAAAATTTCCATCTTTCTCTATTTTAGATGACTGT
TACCCTTGGGATTTGAGTGAAATTTATGATGCGCCTGTACTTTTATTTTACAAGGGAAATCTGGATCTCTTGAAATTCCC
TAAGGTAGCAGTTGTCGGTAGTCGTGCTTGTAGCAAACAGGGAGCTAAGTCAGTTGAAAAAGTCATTCAAGGATTGGAAA
ATGAACTGGTTATCGTCAGTGGATTAGCCAAGGGAATAGACACAGCAGCCCATATGGCAGCTCTTCAAAATGGCGGAAAA
ACCATTGCAGTGATTGGAACAGGACTGGATGTGTTTTATCCTAAAGCCAATAAACGCTTGCAAAACTACATCGGCAATGA
CCATCTGGTTCTCAGTGAATATGGACCTGGCGAACAACCTCTGAAATTTCATTTTCCTGCCCGTAATCGCATCATTGCTG
GACTTTGCCGTGGTGTGATTGTAGCAGAGGCCAAGATGCGCTCAGGTAGTCTCATTACCTGTGAGCGAGCAATGGAAGAA
GGACGCGATGTCTTTGCTATTCCTGGTAGCATTTTAGATGGACTATCAGACGGTTGCCATCATTTGATTCAAGAAGGGGC
AAAATTGGTTACAAGTGGGAAGGATGTTCTTGCGGAATTTGAATTTTAA

Domains

Predicted by InterproScan.

DNA recombination-mediator protein A (DNA_processg_A)

(75-271)

DNA processing protein A sterile alpha motif domain (SAM_DprA)

(3-64)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A387B2Z0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  dprA/cilB/dalA Streptococcus pneumoniae Rx1

98.936

100

0.989
  dprA/cilB/dalA Streptococcus pneumoniae D39

98.936

100

0.989
  dprA/cilB/dalA Streptococcus pneumoniae R6

98.936

100

0.989
  dprA/cilB/dalA Streptococcus pneumoniae TIGR4

98.936

100

0.989
  dprA/cilB/dalA Streptococcus mitis NCTC 12261

98.227

100

0.982
  dprA/cilB/dalA Streptococcus mitis SK321

97.518

100

0.975
  dprA Streptococcus mutans UA159

62.857

99.291

0.624
  dprA Lactococcus lactis subsp. cremoris KW2

55.357

99.291

0.55
  dprA Staphylococcus aureus N315

38.163

100

0.383
  dprA Haemophilus influenzae Rd KW20

38.577

94.681

0.365

Multiple sequence alignment