Detailed information NTG0317180

Bioinformatically predicted

Overview

• Name: comEA/celA/cilE
• Type: Machinery gene
• Locus tag: D7D53_RS03170
• Genome accession: NZ_CP032621
• Coordinates: 635720..636370 (+)
• Length: 216 a.a.
• NCBI ID: WP_120770098.1
• Uniprot ID: A0A387AWS6
• Organism: Streptococcus gwangjuensis strain KCOM 1679 (=ChDC B345)
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 630720..641370

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
D7D53_RS03135 (D7D53_03135)	pyrH	630745..631482 (+)	738	WP_000002996.1	UMP kinase	-
D7D53_RS03140 (D7D53_03140)	frr	631491..632048 (+)	558	WP_001262225.1	ribosome recycling factor	-
D7D53_RS03145 (D7D53_03145)	cvfB	632108..632962 (+)	855	WP_120164223.1	RNA-binding virulence regulatory protein CvfB	-
D7D53_RS03150 (D7D53_03150)	-	632971..633186 (+)	216	WP_120164222.1	YozE family protein	-
D7D53_RS03155 (D7D53_03155)	-	633271..634266 (+)	996	WP_120770096.1	PhoH family protein	-
D7D53_RS03160 (D7D53_03160)	ald	634323..635435 (-)	1113	WP_120770097.1	alanine dehydrogenase	-
D7D53_RS03165 (D7D53_03165)	-	635570..635653 (+)	84	Protein_601	N-acetyltransferase	-
D7D53_RS03170 (D7D53_03170)	comEA/celA/cilE	635720..636370 (+)	651	WP_120770098.1	helix-hairpin-helix domain-containing protein	Machinery gene
D7D53_RS03175 (D7D53_03175)	comEC/celB	636354..638594 (+)	2241	WP_120770099.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
D7D53_RS03180 (D7D53_03180)	infC	638814..639344 (+)	531	WP_000848180.1	translation initiation factor IF-3	-
D7D53_RS03185 (D7D53_03185)	rpmI	639377..639577 (+)	201	WP_001125943.1	50S ribosomal protein L35	-
D7D53_RS03190 (D7D53_03190)	rplT	639629..639988 (+)	360	WP_120770100.1	50S ribosomal protein L20	-
D7D53_RS03195 (D7D53_03195)	-	640047..640427 (+)	381	WP_000157153.1	VOC family protein	-

Sequence

Protein

• Length: 216 a.a.
• Molecular weight: 23171.40 Da
• Isoelectric Point: 5.2380

>NTDB_id=317180 D7D53_RS03170 WP_120770098.1 635720..636370(+) (comEA/celA/cilE) [Streptococcus gwangjuensis strain KCOM 1679 (=ChDC B345)]
MEAIIEKIKEYKIIVICTGLGLLVGGFFLLKPVPHTPVKETNLQAEVAAVSNDSVSEKEVKKEEEEEPVEQDLITVDVKG
AVKSPGIYDLPVGSRVNDAVQKAGGLTEQADSKALNLAQKVSDEALVYVPTKGEESASQQAGSGAPSSTSKEKKVNLNKA
SLEELKQIKGLGGKRAQDIIDHRESNGKFKSVDELKKVSGIGAKTIEKLKDYVTVD

Nucleotide

• Length: 651 bp

>NTDB_id=317180 D7D53_RS03170 WP_120770098.1 635720..636370(+) (comEA/celA/cilE) [Streptococcus gwangjuensis strain KCOM 1679 (=ChDC B345)]
ATGGAAGCAATTATCGAGAAAATCAAAGAGTATAAAATCATTGTCATCTGTACTGGTCTGGGCTTGCTTGTCGGCGGATT
TTTCCTGCTAAAACCAGTTCCACACACACCTGTCAAAGAAACGAATTTGCAGGCTGAAGTTGCAGCTGTTTCCAATGATT
CGGTATCCGAAAAGGAAGTGAAGAAGGAAGAAGAGGAAGAACCTGTTGAACAAGATCTAATCACAGTAGATGTGAAAGGC
GCTGTCAAATCACCAGGGATTTATGATTTGCCTGTAGGTAGTCGGGTCAATGATGCTGTTCAGAAGGCTGGTGGCTTGAC
AGAGCAAGCAGACAGCAAGGCACTCAATCTAGCTCAGAAAGTTAGTGATGAGGCTCTGGTTTACGTTCCAACTAAGGGAG
AAGAATCGGCTAGTCAACAGGCAGGTTCTGGAGCGCCTTCTTCAACAAGCAAGGAAAAGAAGGTAAATCTTAACAAGGCC
AGTCTGGAAGAGCTTAAACAGATTAAGGGACTGGGAGGAAAACGAGCTCAGGACATTATCGACCATCGTGAGTCAAATGG
GAAATTCAAGTCGGTTGATGAACTCAAGAAAGTTTCTGGAATTGGTGCCAAGACCATAGAAAAGTTAAAAGATTATGTTA
CAGTGGATTAA

Domains

Predicted by InterproScan.

• SLBB domain (SLBB) (76-126)

• Helix-hairpin-helix motif (HHH_3) (151-214)

3D structure

• AlphaFold DB: A0A387AWS6

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA/celA/cilE	Streptococcus mitis NCTC 12261	93.981	100	0.94
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	92.593	100	0.926
comEA/celA/cilE	Streptococcus mitis SK321	92.13	100	0.921
comEA/celA/cilE	Streptococcus pneumoniae Rx1	91.667	100	0.917
comEA/celA/cilE	Streptococcus pneumoniae D39	91.667	100	0.917
comEA/celA/cilE	Streptococcus pneumoniae R6	91.667	100	0.917
comEA	Lactococcus lactis subsp. cremoris KW2	40.889	100	0.426