Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   D6Z43_RS22085 Genome accession   NZ_CP032616
Coordinates   4683420..4685522 (+) Length   700 a.a.
NCBI ID   WP_120654170.1    Uniprot ID   A0AAD0TFT1
Organism   Pseudomonas sp. DY-1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4678420..4690522
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  D6Z43_RS22065 (D6Z43_22065) pilM 4680534..4681598 (+) 1065 WP_120654166.1 pilus assembly protein PilM Machinery gene
  D6Z43_RS22070 (D6Z43_22070) pilN 4681598..4682212 (+) 615 WP_120654167.1 type 4a pilus biogenesis protein PilN -
  D6Z43_RS22075 (D6Z43_22075) pilO 4682209..4682832 (+) 624 WP_120654168.1 type 4a pilus biogenesis protein PilO -
  D6Z43_RS22080 (D6Z43_22080) pilP 4682829..4683362 (+) 534 WP_120654169.1 type 4a pilus biogenesis lipoprotein PilP -
  D6Z43_RS22085 (D6Z43_22085) pilQ 4683420..4685522 (+) 2103 WP_120654170.1 type IV pilus secretin PilQ Machinery gene
  D6Z43_RS22090 (D6Z43_22090) aroK 4685528..4686046 (+) 519 WP_077524753.1 shikimate kinase AroK -
  D6Z43_RS22095 (D6Z43_22095) aroB 4686098..4687201 (+) 1104 WP_120654171.1 3-dehydroquinate synthase -
  D6Z43_RS22100 (D6Z43_22100) - 4687211..4688896 (+) 1686 WP_120654172.1 AAA family ATPase -

Sequence

Protein


Download         Length: 700 a.a.        Molecular weight: 75115.15 Da        Isoelectric Point: 4.9248

>NTDB_id=317061 D6Z43_RS22085 WP_120654170.1 4683420..4685522(+) (pilQ) [Pseudomonas sp. DY-1]
MKSSLSRLGITLLAALLSPALLAANLQALDVAALPGDRVELKLAFDEPVTAPRGYTIEQPARIALDLPGVSNKLGSKNRE
LGVGNARSVTVVEAKDRTRLIINLSTLVPYSTRVDGRNLYVLVGDSATSSTAPRPVASAPAPVAAPLTASQSAKSYASTG
RAIENIDFQRGEQGEGNVVITLSDPSVSPDIQEQGGKIRLDFSKTQIPEPLRVRLDVKDFATPVQFVSASGSGSNASISI
EPTGYYDYLAYQTDNKLTVSIKPLTQDDVEKRKTERFAYSGEKLSLNFQDIDVRSVLQLIADFTDLNLVASDTVQGNITL
RLQNVPWDQALDLVLKTKGLDKRKVGNVLLVAPADEIAARERQELESQKQIAELAPLRRELIQVNYAKAADIAKLFQSVT
SGDPSAAGADERGSITVDDRTNSIIAYQTQDKLEELRRIVTQLDIPVRQVMIEARIVEANVDYDKALGVNWRGASIGDNN
FVIGGGGAGRPAANQAGNVNLANAPFVDLGATGATSSIGIGFITDNTILDLELSAMEKTGNGEIVSQPKVVTSDKETAKI
LKGQEVPYQEASSSGATSTSFKEAALSLEVTPQITPDNRIIMEVKVTKDAPDFGNALNGVPPINKNEVNAKVLVNDGETI
VIGGVFSNTQTKAVDKVPFLGDLPFLGRMFRRDVVQDKKSELLVFLTPRIMNNQAIAVSR

Nucleotide


Download         Length: 2103 bp        

>NTDB_id=317061 D6Z43_RS22085 WP_120654170.1 4683420..4685522(+) (pilQ) [Pseudomonas sp. DY-1]
ATGAAAAGCTCGCTATCGCGCCTTGGTATCACGCTATTGGCCGCCCTGCTGTCGCCAGCGCTATTGGCTGCGAACCTGCA
GGCATTGGATGTCGCGGCTCTCCCAGGCGACAGGGTTGAACTCAAACTGGCATTCGACGAGCCCGTGACGGCTCCGCGTG
GCTATACCATCGAGCAGCCTGCGCGTATCGCCCTGGATTTGCCAGGTGTGTCCAACAAGCTGGGTTCCAAGAACCGCGAA
TTGGGTGTAGGGAACGCTCGTAGCGTGACCGTGGTGGAAGCCAAGGATCGCACTCGCCTGATCATCAACCTGTCGACCCT
GGTGCCCTACAGCACCCGTGTTGATGGCCGCAATCTCTATGTGCTGGTGGGCGATTCCGCGACTTCGTCTACAGCGCCTC
GCCCCGTGGCATCCGCCCCGGCTCCCGTGGCCGCGCCCCTGACTGCTTCCCAGTCGGCCAAGAGCTACGCCAGTACTGGG
CGTGCCATCGAGAATATCGACTTCCAGCGCGGCGAGCAGGGTGAGGGTAATGTGGTCATCACCCTGTCAGACCCCAGTGT
CAGCCCGGATATCCAGGAACAGGGCGGAAAGATTCGCCTGGACTTCTCCAAGACTCAGATCCCTGAGCCGCTGCGCGTCC
GTCTCGACGTGAAGGATTTTGCCACTCCGGTGCAGTTCGTCAGCGCTTCCGGCTCCGGCAGTAACGCCAGTATCAGTATC
GAACCCACCGGGTACTACGACTACCTCGCCTACCAGACCGACAACAAACTGACCGTCAGTATCAAGCCGCTGACTCAGGA
TGACGTGGAAAAACGCAAGACTGAGCGTTTCGCCTACTCCGGCGAGAAGCTGTCTCTGAATTTCCAGGACATCGACGTTC
GTTCCGTACTCCAACTGATCGCCGACTTCACCGACCTCAACCTGGTGGCGTCGGATACCGTTCAGGGGAACATCACCCTG
CGGCTGCAGAACGTGCCTTGGGATCAGGCGCTGGATCTGGTCCTGAAGACCAAGGGCCTGGACAAGCGCAAAGTGGGTAA
CGTGTTGCTGGTCGCGCCTGCAGACGAGATCGCTGCCCGCGAACGCCAGGAACTGGAATCGCAGAAACAGATCGCTGAAC
TGGCTCCACTGCGTCGTGAGCTGATCCAGGTGAACTACGCCAAGGCCGCGGACATTGCCAAGCTGTTCCAGTCCGTCACC
AGCGGTGACCCGAGTGCTGCCGGCGCTGATGAGCGCGGCTCCATTACTGTGGATGACCGCACCAACAGCATCATCGCCTA
TCAGACCCAGGACAAACTGGAAGAGCTGCGTCGTATCGTGACCCAGCTGGATATCCCGGTTCGCCAGGTAATGATCGAAG
CGCGCATCGTCGAAGCCAACGTCGACTACGACAAGGCGCTTGGCGTCAACTGGCGTGGTGCGAGCATTGGCGACAACAAC
TTTGTCATCGGTGGCGGTGGTGCCGGCCGTCCGGCTGCCAACCAGGCTGGCAACGTGAACCTGGCCAACGCGCCCTTCGT
CGATCTGGGCGCCACTGGCGCAACCTCGAGCATCGGCATCGGCTTCATTACCGACAACACCATCCTCGACCTTGAGCTGT
CGGCGATGGAAAAGACCGGTAACGGCGAAATCGTATCGCAGCCCAAGGTGGTCACCTCCGACAAGGAAACCGCGAAGATC
CTCAAGGGCCAGGAAGTGCCTTATCAGGAAGCCAGCTCCAGCGGCGCCACCAGCACCTCCTTCAAGGAAGCGGCCCTTTC
CCTTGAAGTGACTCCGCAGATCACTCCGGACAACCGCATCATCATGGAGGTCAAGGTCACCAAGGACGCTCCGGACTTCG
GCAACGCCCTGAACGGTGTGCCGCCGATCAACAAGAACGAGGTAAACGCCAAGGTACTGGTCAATGATGGTGAAACCATC
GTGATAGGTGGGGTATTCTCCAACACCCAAACCAAGGCGGTGGACAAGGTGCCCTTCCTCGGCGACCTGCCCTTCCTCGG
CCGCATGTTCCGTCGCGACGTGGTTCAGGACAAGAAATCCGAGCTCCTGGTATTCCTCACTCCCCGCATTATGAATAACC
AGGCCATTGCGGTGAGTCGCTGA

Domains

Predicted by InterproScan.

Bacterial type II and III secretion system protein (Secretin)

(535-691)

Secretin and TonB N terminus short domain (STN)

(307-353)

AMIN domain (AMIN)

(38-121)

Bacterial type II/III secretion system short domain (Secretin_N)

(381-449)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

77.298

100

0.793
  comQ Acinetobacter baylyi ADP1

43.804

99.143

0.434
  pilQ Acinetobacter baumannii strain A118

41.515

100

0.423
  pilQ Acinetobacter baumannii D1279779

41.515

100

0.423
  pilQ Legionella pneumophila strain Lp02

39.296

97.429

0.383
  pilQ Legionella pneumophila strain ERS1305867

39.15

97.429

0.381

Multiple sequence alignment