Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   D6Z43_RS22065 Genome accession   NZ_CP032616
Coordinates   4680534..4681598 (+) Length   354 a.a.
NCBI ID   WP_120654166.1    Uniprot ID   -
Organism   Pseudomonas sp. DY-1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4675534..4686598
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  D6Z43_RS22055 (D6Z43_22055) - 4676589..4677857 (+) 1269 WP_120654164.1 malic enzyme-like NAD(P)-binding protein -
  D6Z43_RS22060 (D6Z43_22060) - 4677914..4680349 (-) 2436 WP_162945865.1 penicillin-binding protein 1A -
  D6Z43_RS22065 (D6Z43_22065) pilM 4680534..4681598 (+) 1065 WP_120654166.1 pilus assembly protein PilM Machinery gene
  D6Z43_RS22070 (D6Z43_22070) pilN 4681598..4682212 (+) 615 WP_120654167.1 type 4a pilus biogenesis protein PilN -
  D6Z43_RS22075 (D6Z43_22075) pilO 4682209..4682832 (+) 624 WP_120654168.1 type 4a pilus biogenesis protein PilO -
  D6Z43_RS22080 (D6Z43_22080) pilP 4682829..4683362 (+) 534 WP_120654169.1 type 4a pilus biogenesis lipoprotein PilP -
  D6Z43_RS22085 (D6Z43_22085) pilQ 4683420..4685522 (+) 2103 WP_120654170.1 type IV pilus secretin PilQ Machinery gene
  D6Z43_RS22090 (D6Z43_22090) aroK 4685528..4686046 (+) 519 WP_077524753.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 354 a.a.        Molecular weight: 37851.07 Da        Isoelectric Point: 4.4486

>NTDB_id=317060 D6Z43_RS22065 WP_120654166.1 4680534..4681598(+) (pilM) [Pseudomonas sp. DY-1]
MLGLFNKKANSLLGIDISSTSVKLLELSRSGSRFKVESYAVEPLPPNAVVEKNIAELEGVGQALSRVVTKARTGVKAAAV
AVAGSAVITKTIEMEAGLSDDDLENQLKIEADQYIPYPLEEVAIDFEVQGASARNPERVDVLLAACRKENVEVREAALAL
AGLTAKVVDVEAYALERAYSLLSAQLGDSHDELTVAVVDIGATMTTLSVLHNGRTIYTREQLFGGRQLTEEIQRRYGLSV
EEAGLAKKQGGLPDDYDSEVLQPFKDAVVQQVSRSLQFFFAAGQYNDVDYILLAGGTASIPDLDRLIQQKIGTPTLVANP
FADMALSSKVNAGALASDAPALMIACGLAMRSFD

Nucleotide


Download         Length: 1065 bp        

>NTDB_id=317060 D6Z43_RS22065 WP_120654166.1 4680534..4681598(+) (pilM) [Pseudomonas sp. DY-1]
GTGCTAGGGCTCTTCAATAAGAAAGCGAATTCGCTGTTGGGGATCGACATCAGCTCGACCTCAGTCAAGCTCCTTGAATT
GAGTCGCTCGGGAAGCCGCTTCAAAGTGGAGTCCTATGCCGTCGAGCCACTCCCTCCAAATGCGGTTGTCGAAAAGAACA
TCGCGGAACTGGAGGGCGTTGGGCAGGCGCTTTCCCGCGTTGTCACCAAGGCGCGAACCGGGGTCAAGGCCGCTGCGGTC
GCCGTGGCAGGCTCGGCCGTGATTACCAAGACCATCGAGATGGAAGCCGGTCTCTCCGATGATGATCTGGAGAATCAGCT
GAAAATCGAGGCCGACCAATACATTCCCTATCCGTTGGAAGAAGTCGCCATCGACTTCGAGGTGCAAGGCGCCTCGGCTC
GCAATCCGGAGCGGGTCGACGTACTTCTGGCTGCTTGCCGGAAGGAAAACGTCGAAGTACGTGAGGCAGCGCTTGCCCTG
GCAGGACTTACCGCCAAGGTCGTCGATGTCGAGGCTTACGCGTTGGAACGCGCCTATTCACTGCTTTCGGCCCAGCTCGG
TGATTCCCACGACGAACTTACCGTGGCGGTTGTTGATATCGGCGCCACCATGACGACCCTCAGCGTGCTGCACAACGGTC
GTACCATTTATACGCGGGAGCAGCTTTTCGGTGGTCGCCAGTTGACCGAGGAGATTCAGCGTCGCTATGGGCTTTCGGTC
GAGGAAGCTGGTCTTGCCAAGAAGCAAGGCGGTCTTCCGGATGACTACGACAGCGAGGTCCTTCAACCGTTCAAGGATGC
CGTCGTCCAGCAGGTATCCCGATCGCTGCAGTTCTTCTTCGCCGCGGGCCAGTACAACGACGTGGACTACATCCTCCTGG
CCGGCGGTACGGCCTCGATTCCTGATCTGGATCGCCTGATCCAGCAGAAGATCGGTACGCCGACCCTGGTTGCCAACCCC
TTCGCCGACATGGCGCTGAGTAGCAAGGTCAATGCGGGTGCCCTGGCCAGCGATGCGCCTGCGCTGATGATTGCTTGCGG
CTTGGCGATGAGGAGTTTCGACTGA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(14-353)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Acinetobacter baumannii D1279779

56.497

100

0.565
  comM Acinetobacter nosocomialis M2

56.497

100

0.565
  comM Acinetobacter baylyi ADP1

53.39

100

0.534
  pilM Legionella pneumophila strain ERS1305867

46.893

100

0.469

Multiple sequence alignment