Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   D6Z43_RS15025 Genome accession   NZ_CP032616
Coordinates   3187162..3188868 (+) Length   568 a.a.
NCBI ID   WP_120652959.1    Uniprot ID   -
Organism   Pseudomonas sp. DY-1     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3182162..3193868
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  D6Z43_RS14995 (D6Z43_14995) nadC 3183028..3183876 (+) 849 WP_120652954.1 carboxylating nicotinate-nucleotide diphosphorylase -
  D6Z43_RS15005 (D6Z43_15005) - 3184258..3184485 (+) 228 WP_120652956.1 hypothetical protein -
  D6Z43_RS15015 (D6Z43_15015) - 3184683..3186356 (-) 1674 WP_120652957.1 PglL family O-oligosaccharyltransferase -
  D6Z43_RS15020 (D6Z43_15020) - 3186424..3186900 (-) 477 WP_120652958.1 pilin -
  D6Z43_RS15025 (D6Z43_15025) pilB 3187162..3188868 (+) 1707 WP_120652959.1 type IV-A pilus assembly ATPase PilB Machinery gene
  D6Z43_RS15030 (D6Z43_15030) pilC 3188871..3190088 (+) 1218 WP_120652960.1 type II secretion system F family protein Machinery gene
  D6Z43_RS15035 (D6Z43_15035) pilD 3190092..3190964 (+) 873 WP_120652961.1 A24 family peptidase Machinery gene
  D6Z43_RS15040 (D6Z43_15040) coaE 3190961..3191572 (+) 612 WP_120652962.1 dephospho-CoA kinase -
  D6Z43_RS15045 (D6Z43_15045) yacG 3191569..3191769 (+) 201 WP_120652963.1 DNA gyrase inhibitor YacG -
  D6Z43_RS15050 (D6Z43_15050) - 3191890..3192105 (-) 216 WP_120652964.1 hypothetical protein -
  D6Z43_RS15055 (D6Z43_15055) - 3192384..3193073 (-) 690 WP_120652965.1 energy-coupling factor ABC transporter permease -
  D6Z43_RS15060 (D6Z43_15060) - 3193070..3193537 (-) 468 WP_120652966.1 FAD/FMN-containing dehydrogenase -

Sequence

Protein


Download         Length: 568 a.a.        Molecular weight: 62604.64 Da        Isoelectric Point: 5.6053

>NTDB_id=317044 D6Z43_RS15025 WP_120652959.1 3187162..3188868(+) (pilB) [Pseudomonas sp. DY-1]
MTDSAPTLSGLAKQLVITNLLDERSALQAHTQAQRNKIPLITYLVQNKLLKSRDLAEMAAEQFGVAFLDLSSVDKESQPK
DLVSEKLVRQHRALPLWRRGNKLFVGISDPTNHQAVTDIQFSTGLNTEAILVEDDKLGDAIEKFFDSANSGMEDLGDVDL
DGVDVEAVEDDKKDSPGGVDADDAPVVRFVNKMLLDAIRGGSSDLHFEPYERSYRVRFRTDGMLHEVAKPPIQLASRIAA
RLKVMAALDISERRKPQDGRIKMKISKSKSIDFRVNTLPTLWGEKIVMRILDPTSAQMGIDALGYEEEQKELYLAALKQP
QGMILVTGPTGSGKTVSLYTGINILNTVDINISTAEDPVEINLEGINQVNVNPKQGMDFAQALRAFLRQDPDVIMVGEVR
DLETAEIAIKAAQTGHMVLSTLHTNSAAETLTRLRNMGVPAFNIATSVNLIIAQRLARKLCSHCKKEVDVPREVLVKEGF
PEARIGSFKLYGPVGCENCNQGYKGRVGIYEVVKITPAMQQLIMEEGNSIQIAQQARRDGFNDLRTSGLVKAMNGITSLE
EVNRVTKD

Nucleotide


Download         Length: 1707 bp        

>NTDB_id=317044 D6Z43_RS15025 WP_120652959.1 3187162..3188868(+) (pilB) [Pseudomonas sp. DY-1]
ATGACCGACTCCGCCCCTACACTTTCCGGCCTGGCCAAGCAACTGGTCATCACCAATCTGCTAGATGAACGGAGTGCGCT
GCAGGCCCATACGCAGGCGCAGCGCAACAAGATTCCGCTTATCACCTACCTGGTACAGAACAAGCTGCTGAAGAGTCGGG
ATCTGGCGGAAATGGCGGCTGAGCAGTTTGGCGTGGCGTTTCTCGACCTTTCCAGCGTCGACAAGGAATCCCAGCCGAAA
GACCTAGTGAGCGAAAAGCTGGTGCGCCAGCACCGCGCCCTGCCACTCTGGCGCCGTGGCAACAAGCTATTCGTGGGAAT
TTCCGACCCCACCAATCACCAAGCCGTGACCGACATCCAGTTCAGTACCGGCCTCAATACCGAGGCCATCCTGGTTGAGG
ATGACAAGCTGGGCGATGCCATCGAGAAGTTCTTCGACTCCGCCAACAGCGGCATGGAGGACCTCGGTGACGTCGACCTG
GATGGCGTGGATGTCGAGGCAGTCGAAGACGACAAAAAGGACAGCCCCGGTGGCGTGGACGCCGACGACGCGCCGGTGGT
GCGCTTCGTCAACAAGATGCTGCTGGATGCGATCCGCGGCGGCTCCTCGGACCTGCACTTCGAGCCCTATGAGCGCTCCT
ACCGGGTGCGCTTCCGTACCGACGGCATGTTGCATGAAGTGGCCAAGCCGCCGATCCAGCTGGCCAGCCGCATTGCCGCG
CGCCTGAAGGTAATGGCCGCGCTGGATATTTCCGAGCGCCGCAAGCCCCAGGACGGTCGGATCAAGATGAAGATTTCCAA
GTCGAAATCCATCGACTTCCGCGTCAACACCCTACCGACGCTGTGGGGCGAGAAGATCGTGATGCGGATTCTCGACCCCA
CCAGCGCACAGATGGGTATCGACGCCCTGGGGTACGAAGAGGAGCAAAAGGAGCTGTACCTGGCCGCCCTGAAGCAGCCC
CAGGGGATGATCCTGGTCACCGGCCCCACCGGTTCGGGCAAGACGGTATCGCTCTACACCGGCATCAATATCCTCAATAC
CGTGGACATCAACATCTCCACCGCCGAAGACCCGGTGGAGATCAACCTGGAAGGCATCAACCAGGTCAATGTCAACCCGA
AGCAGGGCATGGACTTTGCCCAGGCCCTGCGCGCCTTCCTGCGTCAGGACCCGGACGTGATCATGGTGGGTGAAGTTCGT
GACCTGGAAACCGCCGAGATCGCCATCAAGGCCGCCCAGACTGGTCACATGGTGTTGTCTACCCTGCACACCAACAGTGC
GGCGGAAACCTTGACCCGCCTGCGCAATATGGGCGTGCCGGCCTTTAACATCGCCACCTCGGTCAACCTGATCATCGCCC
AGCGCCTGGCACGCAAATTGTGTAGCCATTGCAAGAAGGAAGTGGACGTGCCGCGTGAGGTGCTGGTCAAGGAAGGCTTT
CCGGAGGCCAGGATCGGCAGCTTCAAGCTGTATGGCCCGGTCGGCTGCGAGAACTGCAACCAGGGCTACAAGGGCCGTGT
GGGTATTTATGAAGTGGTTAAAATCACGCCGGCCATGCAGCAGCTTATTATGGAAGAAGGCAACTCCATCCAGATCGCCC
AGCAGGCGCGCCGAGATGGCTTCAATGACCTGCGAACCTCAGGCCTGGTTAAAGCCATGAACGGCATTACCAGCCTTGAG
GAAGTCAACCGCGTGACCAAGGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(192-459)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(44-148)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

57.923

100

0.579
  pilB Acinetobacter baylyi ADP1

57.672

99.824

0.576
  pilB Legionella pneumophila strain ERS1305867

56.79

99.824

0.567
  pilB Vibrio parahaemolyticus RIMD 2210633

49.291

99.296

0.489
  pilB Vibrio cholerae strain A1552

52.075

93.31

0.486
  pilF Neisseria gonorrhoeae MS11

48.845

99.12

0.484
  pilB Vibrio campbellii strain DS40M4

48.239

100

0.482
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.509

93.134

0.368
  pilF Thermus thermophilus HB27

39.057

93.31

0.364

Multiple sequence alignment