Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   D5S12_RS02000 Genome accession   NZ_CP032459
Coordinates   430829..432934 (+) Length   701 a.a.
NCBI ID   WP_024662382.1    Uniprot ID   -
Organism   Pseudomonas syringae strain CAS02     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 425829..437934
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  D5S12_RS01980 (D5S12_02140) pilM 427998..429062 (+) 1065 WP_003365992.1 pilus assembly protein PilM Machinery gene
  D5S12_RS01985 (D5S12_02145) - 429062..429637 (+) 576 WP_003365994.1 PilN domain-containing protein -
  D5S12_RS01990 (D5S12_02150) pilO 429634..430257 (+) 624 WP_003432147.1 type 4a pilus biogenesis protein PilO -
  D5S12_RS01995 (D5S12_02155) pilP 430254..430781 (+) 528 WP_003350151.1 type 4a pilus biogenesis lipoprotein PilP -
  D5S12_RS02000 (D5S12_02160) pilQ 430829..432934 (+) 2106 WP_024662382.1 type IV pilus secretin PilQ Machinery gene
  D5S12_RS02005 (D5S12_02165) aroK 432939..433457 (+) 519 WP_003365999.1 shikimate kinase AroK -
  D5S12_RS02010 (D5S12_02170) aroB 433573..434676 (+) 1104 WP_044308720.1 3-dehydroquinate synthase -
  D5S12_RS02015 (D5S12_02175) - 434687..436315 (+) 1629 WP_003432141.1 SPOR domain-containing protein -

Sequence

Protein


Download         Length: 701 a.a.        Molecular weight: 75330.68 Da        Isoelectric Point: 5.0976

>NTDB_id=316014 D5S12_RS02000 WP_024662382.1 430829..432934(+) (pilQ) [Pseudomonas syringae strain CAS02]
MTRILSIIGVSLCIAVLAPVLQAANLKTLDVAALPGDRIELKLAFDAPVPAPRGYTTSQPARIALDLPGVTSQLATKSRD
LGTGNARSVVVVQAQDRTRVIINLTTLSPYSSRVDGNNLFVVIGQGAGAAQASQPTTAMGAQRVAVPPPTPASAKPFIPA
GVKAIRNVDFQRGELGEGNVVIDLSNPSIAPDIQEQGGKIRVDFAKTQLPEPLRVRLDVKDFATPVQFVSASASGDKASI
LIEPSGAFDYSAFQTENKLTISVRPLTNEDLDRRAADKPVYTGEKLSLNFQDIDVRSVLQLIADFTNLNLVASDTVQGGI
TLRLQNVPWDQALDLVLKTKGLDKRKVGNVLLVAPADEIAARERQELESLKQIAELAPLRRELLQVNYAKAADIAKLFQS
VTSAESKADERGSITVDDRTNNIIAYQTQERLDELRRIVSQLDIPVRQVMIEARIVEANVDYNKQLGVRWGGSTNTSGSG
KWTTYGLDNNGDEAGNTGSSTTSNVPFVDLGAANATTGIGLGFVTNNTLLDLELSAMEKTGNGEIVSQPKVVTSDKETAK
ILKGTEIPYQESSSSGATTVSFKEASLSLEVTPQITPDNRIIMEVKVTKDEPDYLNAVLGVPPIKKNEVNAKVLISDGET
IVIGGVFSNTQSKVVDKVPFLGDVPYLGRLFRRDVVSESKSELLVFLTPRIMNNQAISVSR

Nucleotide


Download         Length: 2106 bp        

>NTDB_id=316014 D5S12_RS02000 WP_024662382.1 430829..432934(+) (pilQ) [Pseudomonas syringae strain CAS02]
ATGACCCGGATTCTCTCGATTATCGGCGTGTCGTTATGCATCGCGGTGCTGGCGCCGGTTCTCCAGGCTGCCAACCTCAA
GACCCTGGATGTCGCCGCCCTGCCGGGTGACCGCATCGAGCTGAAGCTGGCATTCGACGCCCCGGTGCCGGCACCGCGCG
GTTACACCACGTCGCAACCAGCGCGCATTGCGCTGGATCTGCCGGGTGTTACCAGCCAGTTGGCGACCAAAAGTCGTGAC
CTGGGCACCGGTAATGCGCGCAGTGTGGTTGTGGTACAGGCTCAGGACCGCACCCGGGTGATCATCAACCTGACCACGCT
TTCGCCGTACAGCTCCCGCGTCGATGGCAACAACCTGTTCGTGGTCATCGGCCAGGGTGCCGGCGCCGCGCAAGCTTCCC
AACCGACCACCGCAATGGGTGCGCAACGGGTCGCGGTGCCACCGCCCACGCCTGCATCCGCCAAACCGTTCATCCCCGCA
GGCGTCAAGGCCATCCGCAATGTCGACTTCCAGCGTGGCGAGTTGGGTGAGGGCAATGTGGTCATCGACCTGAGCAACCC
CAGCATTGCCCCGGATATTCAGGAGCAGGGCGGCAAGATCCGCGTGGACTTCGCCAAGACCCAGCTGCCCGAACCGCTGC
GCGTCCGGCTGGACGTCAAGGACTTCGCCACGCCGGTACAGTTCGTCAGTGCTTCGGCGTCGGGTGACAAGGCCAGTATT
CTGATCGAGCCGTCGGGTGCCTTCGACTACTCGGCGTTCCAGACCGAAAACAAGCTGACGATCAGCGTGCGCCCGTTGAC
CAATGAGGATCTGGACCGCCGCGCCGCTGACAAACCGGTGTACACCGGTGAAAAGCTGTCGCTGAACTTCCAGGACATCG
ACGTACGCTCGGTACTGCAACTGATTGCCGACTTCACCAACCTCAATCTGGTGGCCAGTGATACGGTCCAGGGCGGTATC
ACCCTGCGTCTGCAGAATGTGCCATGGGATCAGGCGCTGGACCTGGTGCTGAAAACCAAGGGCCTGGACAAGCGCAAGGT
CGGCAACGTGCTGCTGGTTGCGCCAGCCGATGAAATTGCCGCCCGCGAGCGTCAGGAGCTGGAGTCGCTCAAGCAGATCG
CCGAGCTGGCGCCCCTGCGTCGTGAGCTGCTGCAAGTCAATTACGCCAAGGCTGCCGACATCGCCAAGCTGTTCCAGTCC
GTGACCAGTGCCGAGTCCAAGGCCGACGAGCGCGGTTCGATCACGGTGGACGACCGGACCAACAACATCATTGCCTACCA
GACCCAAGAGCGCCTCGACGAGCTGCGTCGTATCGTGTCGCAACTGGACATCCCGGTACGCCAGGTGATGATCGAGGCGC
GCATCGTCGAGGCCAACGTCGATTACAACAAGCAACTCGGGGTTCGCTGGGGCGGTTCTACCAACACCAGCGGCAGCGGC
AAATGGACAACCTACGGGCTGGATAATAACGGTGACGAGGCCGGTAACACCGGCAGCAGTACTACCTCCAACGTGCCGTT
CGTGGACCTGGGCGCAGCTAATGCGACGACCGGTATCGGCCTGGGCTTCGTGACCAATAACACGCTGCTGGACCTTGAGC
TGAGTGCCATGGAGAAAACCGGTAACGGTGAAATCGTCTCCCAGCCCAAGGTGGTCACCTCCGATAAGGAAACCGCCAAG
ATCCTCAAGGGTACGGAGATCCCGTATCAGGAATCCAGCTCCAGCGGTGCGACCACCGTGAGCTTCAAGGAGGCCTCCCT
GTCGCTTGAGGTAACGCCGCAGATCACGCCGGACAACCGCATCATCATGGAAGTGAAGGTTACCAAGGACGAGCCGGACT
ACCTGAATGCGGTGCTGGGCGTACCGCCGATCAAGAAAAACGAGGTCAACGCCAAGGTGCTGATTTCCGACGGCGAAACC
ATCGTCATCGGTGGTGTGTTCTCGAATACGCAAAGTAAAGTTGTCGATAAAGTGCCATTTCTTGGCGATGTACCGTATCT
TGGCCGGCTTTTCAGGCGCGATGTGGTATCCGAGTCCAAATCCGAGCTGTTGGTATTTCTGACTCCGCGTATCATGAACA
ACCAGGCGATTTCTGTGAGTCGTTGA

Domains

Predicted by InterproScan.

AMIN domain (AMIN)

(38-122)

Bacterial type II and III secretion system protein (Secretin)

(536-692)

Secretin and TonB N terminus short domain (STN)

(309-355)

Bacterial type II/III secretion system short domain (Secretin_N)

(383-448)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

73.056

100

0.75
  comQ Acinetobacter baylyi ADP1

42.241

99.287

0.419
  pilQ Acinetobacter baumannii D1279779

39.592

100

0.415
  pilQ Acinetobacter baumannii strain A118

39.592

100

0.415
  pilQ Legionella pneumophila strain ERS1305867

38.551

98.431

0.379
  pilQ Legionella pneumophila strain Lp02

38.551

98.431

0.379

Multiple sequence alignment