Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   D5S12_RS01980 Genome accession   NZ_CP032459
Coordinates   427998..429062 (+) Length   354 a.a.
NCBI ID   WP_003365992.1    Uniprot ID   A0A193SY06
Organism   Pseudomonas syringae strain CAS02     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 422998..434062
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  D5S12_RS01970 (D5S12_02130) - 423349..424617 (+) 1269 WP_003365988.1 malic enzyme-like NAD(P)-binding protein -
  D5S12_RS01975 (D5S12_02135) - 425347..427788 (-) 2442 WP_410524720.1 penicillin-binding protein 1A -
  D5S12_RS01980 (D5S12_02140) pilM 427998..429062 (+) 1065 WP_003365992.1 pilus assembly protein PilM Machinery gene
  D5S12_RS01985 (D5S12_02145) - 429062..429637 (+) 576 WP_003365994.1 PilN domain-containing protein -
  D5S12_RS01990 (D5S12_02150) pilO 429634..430257 (+) 624 WP_003432147.1 type 4a pilus biogenesis protein PilO -
  D5S12_RS01995 (D5S12_02155) pilP 430254..430781 (+) 528 WP_003350151.1 type 4a pilus biogenesis lipoprotein PilP -
  D5S12_RS02000 (D5S12_02160) pilQ 430829..432934 (+) 2106 WP_024662382.1 type IV pilus secretin PilQ Machinery gene
  D5S12_RS02005 (D5S12_02165) aroK 432939..433457 (+) 519 WP_003365999.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 354 a.a.        Molecular weight: 38104.65 Da        Isoelectric Point: 4.7049

>NTDB_id=316013 D5S12_RS01980 WP_003365992.1 427998..429062(+) (pilM) [Pseudomonas syringae strain CAS02]
MFELFSKKANTLLGIDISSTSVKLLELSRSGTRYKVESYAVEPLPANAVVEKNIAELEGVGQALSRVLVKAKTSVKIVAV
AVAGSAVITKTIEMDAGLSDDDMENQLKLEADQYIPYPLEEVAIDFEVQGYSVRNPERVEVLLAACRKENVEVREAALAL
AGLTARVVDVEAYALERSFGLLAAQLGNGHDELTVAVVDIGATMTTLSVLHHGRIIYTREQLFGGRQLTDEIQRRYGLSM
EEAGLAKKQGGLPDDYVSEVLDPFKEALVQQVSRSLQFFFAAGQYNSVDHIMLAGGTASISGLEHLIQRRIGTPTMVANP
FADMALSAKVNAGALASDAPALMIACGLALRSFD

Nucleotide


Download         Length: 1065 bp        

>NTDB_id=316013 D5S12_RS01980 WP_003365992.1 427998..429062(+) (pilM) [Pseudomonas syringae strain CAS02]
GTGTTCGAACTCTTCAGTAAGAAGGCCAACACCCTTCTAGGGATCGATATTAGCTCCACCTCGGTAAAACTCCTGGAATT
GAGTCGTTCCGGCACCCGTTACAAGGTCGAGTCTTACGCAGTAGAGCCCTTGCCGGCCAACGCTGTCGTCGAAAAGAACA
TTGCCGAACTCGAAGGGGTCGGGCAGGCGTTGTCACGCGTACTCGTCAAGGCCAAGACCAGCGTCAAGATCGTGGCGGTT
GCCGTGGCCGGTTCGGCGGTGATCACCAAGACCATCGAGATGGACGCCGGTCTTTCCGACGATGACATGGAAAACCAGCT
CAAGCTTGAGGCTGACCAGTACATCCCCTATCCGCTGGAAGAAGTCGCCATCGATTTCGAAGTGCAGGGCTACTCGGTGC
GCAACCCCGAGCGCGTCGAGGTGTTGCTGGCTGCCTGCCGCAAGGAGAACGTCGAAGTGCGCGAGGCCGCGCTGGCACTG
GCCGGACTGACCGCGCGCGTCGTCGATGTCGAGGCCTACGCCCTTGAGCGCTCGTTCGGGCTGCTGGCTGCGCAACTGGG
CAACGGCCATGACGAACTGACGGTGGCCGTGGTCGATATCGGCGCCACCATGACCACCCTGAGCGTGCTGCATCACGGCC
GGATCATCTATACCCGCGAGCAGCTGTTTGGCGGGCGCCAGCTGACTGACGAAATCCAGCGCCGCTATGGGCTCTCCATG
GAGGAGGCCGGGCTTGCCAAGAAGCAGGGCGGCTTGCCCGACGACTACGTCAGCGAAGTGCTCGACCCCTTCAAGGAAGC
GCTGGTGCAGCAGGTTTCCCGCTCCCTGCAGTTCTTCTTCGCGGCCGGTCAATACAACTCTGTGGATCACATCATGCTGG
CAGGCGGCACGGCTTCGATTTCAGGGCTGGAGCATCTGATCCAGCGACGCATCGGCACTCCGACCATGGTCGCCAACCCG
TTTGCCGACATGGCCCTGAGCGCCAAGGTCAATGCCGGGGCCCTGGCCAGTGACGCGCCAGCCCTGATGATCGCCTGTGG
TCTGGCGTTGAGGAGCTTCGACTGA

Domains

Predicted by InterProScan.

Type IV pilus assembly protein PilM; (PilM_2)

(14-353)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Acinetobacter baumannii D1279779

55.932

100

0.559
  comM Acinetobacter nosocomialis M2

55.932

100

0.559
  comM Acinetobacter baylyi ADP1

53.955

100

0.54
  pilM Legionella pneumophila strain ERS1305867

46.479

100

0.466

Multiple sequence alignment