Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   D5S10_RS02060 Genome accession   NZ_CP032456
Coordinates   450378..452483 (+) Length   701 a.a.
NCBI ID   WP_005743117.1    Uniprot ID   A0AAJ2NEE0
Organism   Pseudomonas savastanoi strain CAS03     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 445378..457483
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  D5S10_RS02040 (D5S10_02270) pilM 447523..448587 (+) 1065 WP_002551652.1 pilus assembly protein PilM Machinery gene
  D5S10_RS02045 (D5S10_02275) - 448587..449186 (+) 600 WP_004654254.1 PilN domain-containing protein -
  D5S10_RS02050 (D5S10_02280) pilO 449183..449806 (+) 624 WP_002551654.1 type 4a pilus biogenesis protein PilO -
  D5S10_RS02055 (D5S10_02285) pilP 449803..450330 (+) 528 WP_002551655.1 type 4a pilus biogenesis lipoprotein PilP -
  D5S10_RS02060 (D5S10_02290) pilQ 450378..452483 (+) 2106 WP_005743117.1 type IV pilus secretin PilQ Machinery gene
  D5S10_RS02065 (D5S10_02295) aroK 452488..453006 (+) 519 WP_002551657.1 shikimate kinase AroK -
  D5S10_RS02070 (D5S10_02300) aroB 453122..454225 (+) 1104 WP_002551658.1 3-dehydroquinate synthase -
  D5S10_RS02075 (D5S10_02305) - 454236..455855 (+) 1620 WP_005778690.1 SPOR domain-containing protein -

Sequence

Protein


Download         Length: 701 a.a.        Molecular weight: 75471.84 Da        Isoelectric Point: 5.0976

>NTDB_id=315984 D5S10_RS02060 WP_005743117.1 450378..452483(+) (pilQ) [Pseudomonas savastanoi strain CAS03]
MTRIFSIIGVSLCIAMLSPVLQAANLKTLDVAALPGDRIELKLAFDSPVPAPRGYTTAQPARIALDLPGVTSQLATKSRE
LGNGNARSVVVVQAQDRTRVIINLTTLSPYSSRVDGNNLYVVIGQGAAAAQASQPTTAMGAQRVAVPPPTPASAKPFIPA
GVKAIRNVDFQRGELGEGNVIIDLSNPGIAPDIQEQGGKIRVDFAKTQLPEPLRVRLDVKDFATPVQFVSASATGDKASI
LIEPSGAFDYSAFQTENKLTISVRPLTNEDLDRRAADKPVYTGEKLSLNFQDIDVRSVLQLIADFTNLNLVASDTVQGGI
TLRLQNVPWDQALDLVLKTKGLDKRKVGSVLLVAPADEIAARERQELESLKQIAELAPLRRELLQVNYAKAADIAKLFQS
VTSAESKTDERGSITVDDRTNNIIAYQTQDRLDELRRIVSQLDIPVRQVMIEARIVEANVDYNKQLGVRWGGSTNTSGNG
KWTTYGLDNNGDEAGNTGSNVTSNVPFVDMGASGATSGIGLGFVTNNTLLDLELSAMEKTGNGEIVSQPKVVTSDKETAK
ILKGTEIPYQESSSSGATTVSFKEASLSLEVTPQITPDNRIIMEVKVTKDEPDYLNAVLGVPPIKKNEVNAKVLISDGET
IVIGGVFSNTQSKVVDKVPFLGDVPYLGRLFRRDVVSESKSELLVFLTPRIMNNQAISVSR

Nucleotide


Download         Length: 2106 bp        

>NTDB_id=315984 D5S10_RS02060 WP_005743117.1 450378..452483(+) (pilQ) [Pseudomonas savastanoi strain CAS03]
ATGACCCGGATTTTCTCGATTATCGGCGTGTCGTTATGCATCGCGATGCTTTCGCCGGTTCTCCAGGCTGCCAACCTCAA
GACCCTGGATGTCGCCGCCCTGCCGGGTGATCGCATCGAGCTCAAGCTGGCATTCGACTCGCCGGTGCCTGCGCCGCGCG
GTTACACCACGGCGCAACCTGCGCGTATTGCGCTGGACCTGCCGGGCGTGACCAGTCAGTTGGCGACCAAAAGCCGTGAA
CTCGGTAACGGTAACGCACGCAGCGTGGTCGTTGTACAGGCTCAGGACCGCACGCGGGTAATCATTAACCTGACCACGCT
TTCGCCCTACAGCTCCCGCGTCGACGGTAACAACCTTTACGTGGTTATCGGTCAGGGCGCAGCCGCTGCGCAGGCTTCCC
AGCCAACCACCGCGATGGGCGCGCAACGGGTAGCGGTGCCGCCACCCACTCCGGCATCTGCCAAACCGTTTATCCCTGCG
GGCGTCAAGGCCATCAGGAATGTCGATTTCCAGCGCGGCGAGCTGGGTGAAGGCAATGTGATCATAGACTTGAGCAACCC
CGGCATTGCCCCGGATATTCAGGAGCAGGGCGGCAAGATCCGTGTCGACTTCGCCAAGACCCAACTGCCCGAACCGCTGC
GCGTGCGGCTGGACGTCAAGGACTTCGCCACGCCGGTGCAGTTTGTCAGTGCGTCGGCTACGGGCGACAAGGCCAGTATC
CTGATCGAGCCGTCGGGTGCCTTCGACTACTCGGCGTTTCAGACCGAAAACAAGCTGACCATCAGTGTGCGGCCGTTGAC
CAATGAGGATCTGGACCGCCGCGCCGCTGACAAACCGGTGTACACCGGTGAAAAGCTGTCGCTGAACTTCCAGGACATCG
ACGTACGCTCGGTGCTGCAACTGATTGCCGACTTCACCAACCTCAATCTGGTAGCCAGCGACACGGTTCAGGGCGGTATT
ACTCTGCGTCTGCAGAACGTGCCATGGGATCAGGCGCTGGACCTGGTGCTGAAAACCAAGGGCCTGGACAAGCGCAAGGT
CGGCAGCGTTCTGCTGGTCGCGCCCGCCGATGAAATTGCTGCCCGCGAGCGTCAGGAGCTTGAATCGCTCAAGCAGATCG
CCGAGCTGGCGCCCCTGCGTCGTGAATTGTTGCAGGTCAATTACGCCAAGGCTGCCGATATCGCCAAGCTGTTCCAGTCG
GTGACCAGCGCCGAGTCCAAGACCGACGAGCGTGGCTCGATCACTGTGGACGACCGGACCAACAACATCATTGCCTATCA
AACCCAGGACCGGCTCGACGAACTGCGTCGCATCGTCTCGCAACTGGACATTCCGGTGCGTCAGGTGATGATCGAGGCGC
GCATCGTCGAGGCCAACGTCGATTACAACAAGCAACTGGGTGTGCGCTGGGGCGGCTCGACCAACACCAGCGGCAATGGA
AAATGGACAACCTATGGCCTTGATAATAATGGCGATGAGGCAGGTAACACTGGCAGCAACGTGACCTCCAACGTGCCGTT
TGTCGATATGGGCGCATCTGGCGCAACCTCCGGCATTGGCCTGGGCTTCGTCACCAACAATACGTTACTGGACCTTGAGC
TGAGCGCGATGGAGAAGACCGGTAACGGTGAAATCGTCTCCCAGCCCAAGGTAGTCACTTCCGACAAGGAAACCGCCAAG
ATCCTCAAGGGCACCGAGATCCCGTATCAGGAATCCAGCTCCAGCGGTGCAACCACGGTGAGCTTCAAGGAAGCCTCGCT
GTCGCTTGAGGTAACGCCGCAGATCACGCCGGACAACCGCATCATCATGGAGGTCAAGGTCACCAAGGATGAGCCGGACT
ACCTGAATGCGGTGCTTGGTGTACCACCCATCAAGAAAAACGAGGTCAACGCCAAAGTGCTGATTTCCGATGGCGAAACC
ATCGTCATTGGTGGTGTGTTCTCAAATACGCAAAGTAAAGTTGTCGATAAAGTGCCATTTCTTGGCGATGTACCGTATCT
TGGCCGGCTTTTCAGGCGCGATGTGGTATCCGAGTCCAAATCCGAGCTGTTGGTATTTTTGACTCCGCGTATCATGAACA
ACCAGGCGATTTCTGTGAGTCGTTGA

Domains

Predicted by InterproScan.

Bacterial type II/III secretion system short domain (Secretin_N)

(383-448)

AMIN domain (AMIN)

(38-122)

Secretin and TonB N terminus short domain (STN)

(309-355)

Bacterial type II and III secretion system protein (Secretin)

(536-692)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

72.199

100

0.745
  comQ Acinetobacter baylyi ADP1

40.822

100

0.425
  pilQ Acinetobacter baumannii D1279779

38.255

100

0.407
  pilQ Acinetobacter baumannii strain A118

38.255

100

0.407
  pilQ Legionella pneumophila strain Lp02

38.841

98.431

0.382
  pilQ Legionella pneumophila strain ERS1305867

38.752

98.288

0.381

Multiple sequence alignment